Tokyovirus A1
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 470 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A146JD21|A0A146JD21_9VIRU Uncharacterized protein OS=Tokyovirus A1 OX=1826170 PE=4 SV=1
MM1 pKa = 6.86 STVEE5 pKa = 3.94 ILSNVDD11 pKa = 4.99 LEE13 pKa = 4.52 TTYY16 pKa = 11.38 NGDD19 pKa = 4.34 EE20 pKa = 4.04 EE21 pKa = 4.34 FQANKK26 pKa = 10.18 GLLWEE31 pKa = 5.33 DD32 pKa = 4.17 YY33 pKa = 10.67 ISLEE37 pKa = 4.13 LMCVQQQIQDD47 pKa = 3.74 VKK49 pKa = 11.27 RR50 pKa = 11.84 EE51 pKa = 4.08 LQEE54 pKa = 4.12 LLDD57 pKa = 4.15 MEE59 pKa = 4.85 TDD61 pKa = 3.66 LEE63 pKa = 4.5 FACVFF68 pKa = 3.91
Molecular weight: 7.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.839
IPC2_protein 3.668
IPC_protein 3.554
Toseland 3.389
ProMoST 3.732
Dawson 3.541
Bjellqvist 3.706
Wikipedia 3.465
Rodwell 3.401
Grimsley 3.312
Solomon 3.503
Lehninger 3.452
Nozaki 3.681
DTASelect 3.795
Thurlkill 3.452
EMBOSS 3.478
Sillero 3.681
Patrickios 1.786
IPC_peptide 3.503
IPC2_peptide 3.656
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|A0A146JFH0|A0A146JFH0_9VIRU Uncharacterized protein OS=Tokyovirus A1 OX=1826170 PE=4 SV=1
MM1 pKa = 7.45 EE2 pKa = 5.83 ALPNEE7 pKa = 4.42 TLFHH11 pKa = 6.59 ILYY14 pKa = 10.14 FCGSQEE20 pKa = 3.95 LKK22 pKa = 10.85 NFEE25 pKa = 4.03 RR26 pKa = 11.84 TCRR29 pKa = 11.84 SNRR32 pKa = 11.84 DD33 pKa = 3.33 VLRR36 pKa = 11.84 CGSFWSLYY44 pKa = 9.49 ARR46 pKa = 11.84 EE47 pKa = 4.2 RR48 pKa = 11.84 KK49 pKa = 9.41 HH50 pKa = 5.89 PKK52 pKa = 9.46 KK53 pKa = 10.3 PEE55 pKa = 4.36 NISQKK60 pKa = 8.81 EE61 pKa = 3.61 WSLRR65 pKa = 11.84 MEE67 pKa = 4.28 TQVSVNVKK75 pKa = 9.62 NLMGEE80 pKa = 3.86 PHH82 pKa = 6.76 FGYY85 pKa = 9.49 PSSSTRR91 pKa = 11.84 VLCQRR96 pKa = 11.84 DD97 pKa = 3.52 SARR100 pKa = 11.84 CLIDD104 pKa = 4.6 AISRR108 pKa = 11.84 LTRR111 pKa = 11.84 FAPHH115 pKa = 7.0 NILLYY120 pKa = 10.93 AGGNLLDD127 pKa = 3.84 KK128 pKa = 11.09 NMPVRR133 pKa = 11.84 GKK135 pKa = 10.56 RR136 pKa = 11.84 FIRR139 pKa = 11.84 KK140 pKa = 8.75 SRR142 pKa = 11.84 LRR144 pKa = 11.84 EE145 pKa = 3.77 DD146 pKa = 3.95 RR147 pKa = 11.84 RR148 pKa = 11.84 VKK150 pKa = 10.43 PIGISFVLII159 pKa = 3.94
Molecular weight: 18.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 9.385
IPC_protein 9.633
Toseland 10.321
ProMoST 9.94
Dawson 10.452
Bjellqvist 10.131
Wikipedia 10.613
Rodwell 10.789
Grimsley 10.496
Solomon 10.511
Lehninger 10.482
Nozaki 10.365
DTASelect 10.116
Thurlkill 10.335
EMBOSS 10.716
Sillero 10.379
Patrickios 10.496
IPC_peptide 10.511
IPC2_peptide 9.194
IPC2.peptide.svr19 8.369
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
470
0
470
107735
33
1536
229.2
26.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.405 ± 0.121
2.565 ± 0.099
4.73 ± 0.087
8.714 ± 0.178
5.662 ± 0.103
6.153 ± 0.124
1.775 ± 0.064
4.729 ± 0.073
8.659 ± 0.197
8.869 ± 0.117
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.095 ± 0.059
3.877 ± 0.084
3.966 ± 0.094
3.333 ± 0.098
5.567 ± 0.108
7.822 ± 0.149
5.162 ± 0.146
6.471 ± 0.087
1.561 ± 0.049
2.885 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here