Melbournevirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Marseilleviridae; Marseillevirus; unclassified Marseillevirus

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097I1F7|A0A097I1F7_9VIRU Uncharacterized protein OS=Melbournevirus OX=1560514 GN=MEL_044 PE=4 SV=1
MM1 pKa = 7.16SLVRR5 pKa = 11.84GFFKK9 pKa = 10.57DD10 pKa = 3.24IPIVGSYY17 pKa = 10.72SVLPPVLPRR26 pKa = 11.84GSLVFLTTDD35 pKa = 2.98QNLYY39 pKa = 10.15ISNGQIWAVAGGDD52 pKa = 3.84VGPLAAQVAQNTADD66 pKa = 3.38IATLQTDD73 pKa = 3.63VTTLDD78 pKa = 3.56GQVATNTSNITALQGQVATNTSDD101 pKa = 2.77IGTLQGQVATNTTDD115 pKa = 2.52ITTLQGQVATNTSNITTLQGQVATNTSNITTLQGQVATNTSDD157 pKa = 2.76ITSNTNSINNIIVSQSQGFTIADD180 pKa = 3.64SAQLRR185 pKa = 11.84TFTNPITRR193 pKa = 11.84IDD195 pKa = 3.71VSSTSPTVIYY205 pKa = 10.0TLGTPLDD212 pKa = 3.85GSFNGVYY219 pKa = 10.54LIQWNILAHH228 pKa = 5.22YY229 pKa = 7.21QTANVFYY236 pKa = 10.62AVGQSSVLSNGIGALVSVFGTSTNQTNSSGSSVITVTADD275 pKa = 2.85INLNNYY281 pKa = 9.3RR282 pKa = 11.84LIVTSDD288 pKa = 3.3SAVTTTYY295 pKa = 10.17SGYY298 pKa = 10.92VIVTRR303 pKa = 11.84ILTISPP309 pKa = 3.78

Molecular weight: 32.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097I1M7|A0A097I1M7_9VIRU Uncharacterized protein OS=Melbournevirus OX=1560514 GN=MEL_128 PE=4 SV=1
MM1 pKa = 7.78GYY3 pKa = 10.85LFGRR7 pKa = 11.84SWKK10 pKa = 9.63QKK12 pKa = 9.72RR13 pKa = 11.84IQNGKK18 pKa = 9.08SLGYY22 pKa = 8.83IQKK25 pKa = 9.8SRR27 pKa = 11.84SRR29 pKa = 11.84TTLPVLNSLLFLCFSQNISSPGKK52 pKa = 9.38EE53 pKa = 3.69RR54 pKa = 11.84PKK56 pKa = 9.83KK57 pKa = 8.39TRR59 pKa = 11.84KK60 pKa = 9.37LLYY63 pKa = 10.12GNVRR67 pKa = 11.84KK68 pKa = 10.0KK69 pKa = 11.07LPDD72 pKa = 3.31AALYY76 pKa = 10.0FKK78 pKa = 11.25GPFYY82 pKa = 11.0FLLFLRR88 pKa = 11.84FSFFSSFVDD97 pKa = 3.55LFFVYY102 pKa = 10.3SVPLPVPLCHH112 pKa = 6.89SFFIFFSVCSLSFRR126 pKa = 11.84SRR128 pKa = 11.84LCFALFHH135 pKa = 6.62IGCRR139 pKa = 11.84WKK141 pKa = 9.59TVTT144 pKa = 4.72

Molecular weight: 16.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

448

0

448

110121

50

1537

245.8

28.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.144 ± 0.123

2.532 ± 0.094

4.6 ± 0.081

8.86 ± 0.159

5.787 ± 0.104

6.277 ± 0.124

1.84 ± 0.06

4.834 ± 0.082

8.7 ± 0.184

8.898 ± 0.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.046

3.832 ± 0.087

3.998 ± 0.101

3.341 ± 0.078

5.717 ± 0.102

7.772 ± 0.147

4.925 ± 0.129

6.338 ± 0.099

1.569 ± 0.046

3.012 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski