Beatrice Hill virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Tibrovirus; Beatrice Hill tibrovirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0F5D8|A0A1J0F5D8_9RHAB Nucleocapsid protein OS=Beatrice Hill virus OX=1819301 GN=N PE=4 SV=1
MM1 pKa = 7.18KK2 pKa = 9.89RR3 pKa = 11.84HH4 pKa = 6.68RR5 pKa = 11.84ISIPYY10 pKa = 8.38VTDD13 pKa = 3.12QVLRR17 pKa = 11.84NTSDD21 pKa = 3.38AVDD24 pKa = 3.9PNEE27 pKa = 5.63VIDD30 pKa = 3.99QQISDD35 pKa = 5.4DD36 pKa = 3.63ITNPKK41 pKa = 9.74QDD43 pKa = 3.78LKK45 pKa = 11.25EE46 pKa = 4.04FLDD49 pKa = 3.88TRR51 pKa = 11.84EE52 pKa = 3.81LNYY55 pKa = 9.41RR56 pKa = 11.84TRR58 pKa = 11.84ASSASSYY65 pKa = 11.67DD66 pKa = 3.49DD67 pKa = 5.82DD68 pKa = 5.01DD69 pKa = 4.0WADD72 pKa = 3.85SIVDD76 pKa = 3.93LSRR79 pKa = 11.84KK80 pKa = 8.08SQHH83 pKa = 6.56KK84 pKa = 10.5SDD86 pKa = 5.55GEE88 pKa = 4.27DD89 pKa = 3.26NPQKK93 pKa = 10.97SSGGPNLVLPSISSGVQDD111 pKa = 3.26KK112 pKa = 10.97CSPNTTKK119 pKa = 10.8DD120 pKa = 3.19NKK122 pKa = 9.85IYY124 pKa = 10.82KK125 pKa = 9.5VDD127 pKa = 4.25RR128 pKa = 11.84EE129 pKa = 4.37DD130 pKa = 4.92NDD132 pKa = 3.47MGPNIHH138 pKa = 6.58QIPEE142 pKa = 3.89NRR144 pKa = 11.84PVYY147 pKa = 9.5PSQMYY152 pKa = 9.64PLLEE156 pKa = 4.1VPGNYY161 pKa = 10.05NLLIPKK167 pKa = 9.46LQFFFKK173 pKa = 10.87YY174 pKa = 9.84YY175 pKa = 11.05GLYY178 pKa = 10.24EE179 pKa = 4.36DD180 pKa = 3.76TDD182 pKa = 4.19YY183 pKa = 11.84VVDD186 pKa = 4.79KK187 pKa = 10.89DD188 pKa = 3.56NQGYY192 pKa = 8.78YY193 pKa = 10.35FYY195 pKa = 8.72PTKK198 pKa = 10.51KK199 pKa = 7.37WTTRR203 pKa = 11.84DD204 pKa = 3.2QKK206 pKa = 11.64EE207 pKa = 4.2MFEE210 pKa = 4.32CTPKK214 pKa = 10.57KK215 pKa = 10.56EE216 pKa = 4.21EE217 pKa = 4.26NEE219 pKa = 3.78DD220 pKa = 3.72DD221 pKa = 4.06ALLNLEE227 pKa = 5.57DD228 pKa = 4.86INDD231 pKa = 4.64DD232 pKa = 4.01GPFDD236 pKa = 4.56EE237 pKa = 5.82DD238 pKa = 4.39PEE240 pKa = 4.34VHH242 pKa = 6.28QLIDD246 pKa = 4.56FITKK250 pKa = 9.52GFYY253 pKa = 9.64VEE255 pKa = 4.03KK256 pKa = 10.59RR257 pKa = 11.84NIKK260 pKa = 7.78GTYY263 pKa = 9.5YY264 pKa = 10.8FDD266 pKa = 4.05INNPSLNVNKK276 pKa = 9.93IANVDD281 pKa = 3.78CQNKK285 pKa = 8.78VLSVKK290 pKa = 10.28EE291 pKa = 4.12KK292 pKa = 10.16IDD294 pKa = 4.04MIFKK298 pKa = 10.68ASGIYY303 pKa = 9.93RR304 pKa = 11.84AMKK307 pKa = 10.51LKK309 pKa = 10.82AKK311 pKa = 8.7WW312 pKa = 3.09

Molecular weight: 36.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0F5M1|A0A1J0F5M1_9RHAB U3 protein OS=Beatrice Hill virus OX=1819301 GN=U3 PE=4 SV=1
MM1 pKa = 7.08TLSMDD6 pKa = 3.64RR7 pKa = 11.84LGTEE11 pKa = 4.15IFTKK15 pKa = 9.95IYY17 pKa = 10.36FKK19 pKa = 10.62IKK21 pKa = 10.38EE22 pKa = 4.55DD23 pKa = 3.73FTGWAYY29 pKa = 11.22NLWFKK34 pKa = 10.82IKK36 pKa = 10.61AIVLVLIIILVFVVTIKK53 pKa = 10.71LFKK56 pKa = 10.26SCAYY60 pKa = 9.84VINLIKK66 pKa = 10.67NCCKK70 pKa = 10.08GIAMLRR76 pKa = 11.84HH77 pKa = 6.53KK78 pKa = 10.34GRR80 pKa = 11.84KK81 pKa = 7.85ILSRR85 pKa = 11.84YY86 pKa = 9.45RR87 pKa = 11.84SDD89 pKa = 3.85RR90 pKa = 11.84KK91 pKa = 10.47KK92 pKa = 10.84EE93 pKa = 3.96GNKK96 pKa = 10.08KK97 pKa = 9.74ILIEE101 pKa = 4.19SPIYY105 pKa = 9.54MNNFGFQSDD114 pKa = 4.01TKK116 pKa = 10.95SS117 pKa = 2.97

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4214

117

2116

526.8

60.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.346 ± 0.276

2.207 ± 0.256

6.431 ± 0.856

5.221 ± 0.206

4.438 ± 0.373

5.031 ± 0.285

2.729 ± 0.448

8.234 ± 0.419

7.309 ± 0.776

10.228 ± 0.978

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.249

7.048 ± 0.296

3.963 ± 0.324

3.037 ± 0.269

4.034 ± 0.208

7.712 ± 0.53

5.529 ± 0.331

4.865 ± 0.372

1.614 ± 0.121

4.722 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski