Ostreococcus tauri virus RT-2011

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8ZJ96|H8ZJ96_9PHYC Uncharacterized protein OS=Ostreococcus tauri virus RT-2011 OX=1120767 GN=OtV6_099c PE=4 SV=1
MM1 pKa = 7.57NMQTGFGDD9 pKa = 3.67AGDD12 pKa = 4.36GMLEE16 pKa = 3.93QYY18 pKa = 7.96MKK20 pKa = 11.48SMIDD24 pKa = 3.51IIQPVMEE31 pKa = 4.66KK32 pKa = 11.14AMILAAEE39 pKa = 4.16YY40 pKa = 11.0SKK42 pKa = 11.65ACGRR46 pKa = 11.84DD47 pKa = 3.3TILPEE52 pKa = 4.25DD53 pKa = 3.66MEE55 pKa = 4.66YY56 pKa = 11.51AMKK59 pKa = 10.01YY60 pKa = 10.02CAMNTVGEE68 pKa = 4.53TIGSIMPEE76 pKa = 3.75IYY78 pKa = 10.39DD79 pKa = 4.18EE80 pKa = 4.32EE81 pKa = 5.69LSDD84 pKa = 3.76EE85 pKa = 4.47DD86 pKa = 4.82EE87 pKa = 4.38EE88 pKa = 5.57LEE90 pKa = 4.64EE91 pKa = 5.76IDD93 pKa = 6.13PEE95 pKa = 4.38DD96 pKa = 4.47CPDD99 pKa = 3.85FVRR102 pKa = 11.84YY103 pKa = 10.24SGTDD107 pKa = 3.13PNFIMVNEE115 pKa = 4.23AVDD118 pKa = 4.23RR119 pKa = 11.84WDD121 pKa = 2.99NWVPQNPTEE130 pKa = 4.27QMLKK134 pKa = 10.46NAINSNEE141 pKa = 3.97YY142 pKa = 10.01IGAGGVDD149 pKa = 4.0DD150 pKa = 4.86FF151 pKa = 5.83

Molecular weight: 17.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8ZJC0|H8ZJC0_9PHYC Uncharacterized protein OS=Ostreococcus tauri virus RT-2011 OX=1120767 GN=OtV6_123c PE=4 SV=1
MM1 pKa = 7.43LPDD4 pKa = 3.11ITSQKK9 pKa = 9.25VAVPAALFLALSPGVLLTTNGQKK32 pKa = 10.52LAFRR36 pKa = 11.84NGKK39 pKa = 6.49TNQMAVFFHH48 pKa = 6.73ALVFFLVYY56 pKa = 10.7SLIARR61 pKa = 11.84AMGLVLTKK69 pKa = 10.17TDD71 pKa = 4.99LIVTTVLFLALSPGLLLTLPPGSGGVLRR99 pKa = 11.84SGQTSIPAAVTHH111 pKa = 6.06AVVFAVVFALLRR123 pKa = 11.84KK124 pKa = 9.18QFPQFYY130 pKa = 10.47

Molecular weight: 13.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

249

0

249

59376

66

2671

238.5

27.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.497 ± 0.199

1.679 ± 0.134

6.061 ± 0.167

6.683 ± 0.195

4.362 ± 0.157

5.945 ± 0.223

2.141 ± 0.125

6.327 ± 0.178

7.963 ± 0.41

8.182 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.015 ± 0.103

5.822 ± 0.352

4.177 ± 0.179

3.276 ± 0.139

4.909 ± 0.249

6.026 ± 0.19

6.135 ± 0.222

6.733 ± 0.204

1.056 ± 0.063

4.01 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski