Amino acid dipepetide frequency for Microbacterium phage Count

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.375AlaAla: 8.375 ± 0.974
0.285AlaCys: 0.285 ± 0.108
4.594AlaAsp: 4.594 ± 0.5
5.041AlaGlu: 5.041 ± 0.515
3.537AlaPhe: 3.537 ± 0.344
5.082AlaGly: 5.082 ± 0.697
1.26AlaHis: 1.26 ± 0.221
4.919AlaIle: 4.919 ± 0.443
5.489AlaLys: 5.489 ± 0.543
6.708AlaLeu: 6.708 ± 0.873
2.521AlaMet: 2.521 ± 0.445
3.74AlaAsn: 3.74 ± 0.489
2.765AlaPro: 2.765 ± 0.34
3.415AlaGln: 3.415 ± 0.354
5.407AlaArg: 5.407 ± 0.574
4.635AlaSer: 4.635 ± 0.599
5.57AlaThr: 5.57 ± 0.666
5.529AlaVal: 5.529 ± 0.588
0.935AlaTrp: 0.935 ± 0.15
3.171AlaTyr: 3.171 ± 0.275
0.0AlaXaa: 0.0 ± 0.0
Cys
0.447CysAla: 0.447 ± 0.2
0.081CysCys: 0.081 ± 0.062
0.366CysAsp: 0.366 ± 0.147
0.447CysGlu: 0.447 ± 0.147
0.203CysPhe: 0.203 ± 0.096
0.61CysGly: 0.61 ± 0.191
0.203CysHis: 0.203 ± 0.074
0.244CysIle: 0.244 ± 0.09
0.122CysLys: 0.122 ± 0.065
0.447CysLeu: 0.447 ± 0.155
0.163CysMet: 0.163 ± 0.076
0.163CysAsn: 0.163 ± 0.091
0.203CysPro: 0.203 ± 0.093
0.244CysGln: 0.244 ± 0.097
0.203CysArg: 0.203 ± 0.107
0.407CysSer: 0.407 ± 0.135
0.244CysThr: 0.244 ± 0.112
0.488CysVal: 0.488 ± 0.186
0.163CysTrp: 0.163 ± 0.072
0.081CysTyr: 0.081 ± 0.052
0.0CysXaa: 0.0 ± 0.0
Asp
4.757AspAla: 4.757 ± 0.331
0.244AspCys: 0.244 ± 0.098
4.228AspAsp: 4.228 ± 0.656
5.163AspGlu: 5.163 ± 0.447
3.131AspPhe: 3.131 ± 0.354
5.326AspGly: 5.326 ± 0.653
1.057AspHis: 1.057 ± 0.195
4.757AspIle: 4.757 ± 0.46
3.456AspLys: 3.456 ± 0.397
5.367AspLeu: 5.367 ± 0.544
1.342AspMet: 1.342 ± 0.233
2.805AspAsn: 2.805 ± 0.358
3.131AspPro: 3.131 ± 0.4
2.033AspGln: 2.033 ± 0.269
2.602AspArg: 2.602 ± 0.369
4.066AspSer: 4.066 ± 0.37
3.253AspThr: 3.253 ± 0.369
3.944AspVal: 3.944 ± 0.374
1.016AspTrp: 1.016 ± 0.22
1.708AspTyr: 1.708 ± 0.242
0.0AspXaa: 0.0 ± 0.0
Glu
4.757GluAla: 4.757 ± 0.428
0.325GluCys: 0.325 ± 0.132
4.432GluAsp: 4.432 ± 0.455
5.855GluGlu: 5.855 ± 0.757
3.537GluPhe: 3.537 ± 0.364
4.594GluGly: 4.594 ± 0.395
1.423GluHis: 1.423 ± 0.284
4.676GluIle: 4.676 ± 0.473
4.635GluLys: 4.635 ± 0.618
5.204GluLeu: 5.204 ± 0.5
2.155GluMet: 2.155 ± 0.309
3.822GluAsn: 3.822 ± 0.4
2.033GluPro: 2.033 ± 0.27
2.846GluGln: 2.846 ± 0.383
3.74GluArg: 3.74 ± 0.521
3.456GluSer: 3.456 ± 0.436
3.822GluThr: 3.822 ± 0.392
5.57GluVal: 5.57 ± 0.61
1.504GluTrp: 1.504 ± 0.237
1.952GluTyr: 1.952 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
2.724PheAla: 2.724 ± 0.271
0.325PheCys: 0.325 ± 0.119
3.293PheAsp: 3.293 ± 0.419
3.293PheGlu: 3.293 ± 0.424
1.382PhePhe: 1.382 ± 0.222
3.375PheGly: 3.375 ± 0.341
0.772PheHis: 0.772 ± 0.15
2.074PheIle: 2.074 ± 0.319
1.952PheLys: 1.952 ± 0.328
2.846PheLeu: 2.846 ± 0.31
0.976PheMet: 0.976 ± 0.203
1.504PheAsn: 1.504 ± 0.349
1.464PhePro: 1.464 ± 0.198
0.976PheGln: 0.976 ± 0.185
2.195PheArg: 2.195 ± 0.35
2.358PheSer: 2.358 ± 0.318
2.358PheThr: 2.358 ± 0.359
2.521PheVal: 2.521 ± 0.374
0.61PheTrp: 0.61 ± 0.16
1.382PheTyr: 1.382 ± 0.252
0.0PheXaa: 0.0 ± 0.0
Gly
6.302GlyAla: 6.302 ± 0.794
0.285GlyCys: 0.285 ± 0.114
5.082GlyAsp: 5.082 ± 0.579
4.635GlyGlu: 4.635 ± 0.371
2.724GlyPhe: 2.724 ± 0.268
5.082GlyGly: 5.082 ± 0.628
1.545GlyHis: 1.545 ± 0.306
4.188GlyIle: 4.188 ± 0.548
3.375GlyLys: 3.375 ± 0.294
5.285GlyLeu: 5.285 ± 0.581
1.992GlyMet: 1.992 ± 0.338
2.683GlyAsn: 2.683 ± 0.385
2.195GlyPro: 2.195 ± 0.314
1.83GlyGln: 1.83 ± 0.365
3.781GlyArg: 3.781 ± 0.326
5.611GlySer: 5.611 ± 0.674
5.895GlyThr: 5.895 ± 0.79
5.692GlyVal: 5.692 ± 0.597
1.545GlyTrp: 1.545 ± 0.216
3.537GlyTyr: 3.537 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
1.26HisAla: 1.26 ± 0.304
0.163HisCys: 0.163 ± 0.076
0.813HisAsp: 0.813 ± 0.191
1.22HisGlu: 1.22 ± 0.216
0.732HisPhe: 0.732 ± 0.159
1.748HisGly: 1.748 ± 0.335
0.569HisHis: 0.569 ± 0.154
1.342HisIle: 1.342 ± 0.283
1.016HisLys: 1.016 ± 0.224
1.504HisLeu: 1.504 ± 0.276
0.569HisMet: 0.569 ± 0.152
0.772HisAsn: 0.772 ± 0.184
0.935HisPro: 0.935 ± 0.225
0.976HisGln: 0.976 ± 0.223
1.138HisArg: 1.138 ± 0.256
0.854HisSer: 0.854 ± 0.235
0.894HisThr: 0.894 ± 0.187
1.057HisVal: 1.057 ± 0.191
0.285HisTrp: 0.285 ± 0.125
0.732HisTyr: 0.732 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
4.594IleAla: 4.594 ± 0.565
0.407IleCys: 0.407 ± 0.128
4.188IleAsp: 4.188 ± 0.434
4.269IleGlu: 4.269 ± 0.377
2.765IlePhe: 2.765 ± 0.409
3.7IleGly: 3.7 ± 0.752
1.301IleHis: 1.301 ± 0.254
2.927IleIle: 2.927 ± 0.328
3.456IleLys: 3.456 ± 0.373
5.529IleLeu: 5.529 ± 0.447
1.179IleMet: 1.179 ± 0.216
2.358IleAsn: 2.358 ± 0.352
3.293IlePro: 3.293 ± 0.345
2.561IleGln: 2.561 ± 0.287
3.375IleArg: 3.375 ± 0.371
4.31IleSer: 4.31 ± 0.474
3.131IleThr: 3.131 ± 0.361
3.618IleVal: 3.618 ± 0.352
1.098IleTrp: 1.098 ± 0.197
1.911IleTyr: 1.911 ± 0.303
0.0IleXaa: 0.0 ± 0.0
Lys
4.798LysAla: 4.798 ± 0.494
0.366LysCys: 0.366 ± 0.137
3.334LysAsp: 3.334 ± 0.392
4.188LysGlu: 4.188 ± 0.531
1.992LysPhe: 1.992 ± 0.348
4.025LysGly: 4.025 ± 0.472
1.545LysHis: 1.545 ± 0.238
2.846LysIle: 2.846 ± 0.342
4.391LysLys: 4.391 ± 0.472
4.147LysLeu: 4.147 ± 0.512
2.236LysMet: 2.236 ± 0.258
3.212LysAsn: 3.212 ± 0.435
2.643LysPro: 2.643 ± 0.25
2.155LysGln: 2.155 ± 0.405
3.415LysArg: 3.415 ± 0.412
3.822LysSer: 3.822 ± 0.468
3.456LysThr: 3.456 ± 0.448
4.025LysVal: 4.025 ± 0.419
0.813LysTrp: 0.813 ± 0.161
1.504LysTyr: 1.504 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
6.546LeuAla: 6.546 ± 0.764
0.732LeuCys: 0.732 ± 0.205
5.123LeuAsp: 5.123 ± 0.494
5.57LeuGlu: 5.57 ± 0.564
2.887LeuPhe: 2.887 ± 0.404
5.936LeuGly: 5.936 ± 0.62
1.179LeuHis: 1.179 ± 0.254
4.757LeuIle: 4.757 ± 0.438
4.716LeuLys: 4.716 ± 0.485
5.489LeuLeu: 5.489 ± 0.664
2.195LeuMet: 2.195 ± 0.318
4.147LeuAsn: 4.147 ± 0.442
2.439LeuPro: 2.439 ± 0.326
2.195LeuGln: 2.195 ± 0.317
4.554LeuArg: 4.554 ± 0.478
4.716LeuSer: 4.716 ± 0.478
5.895LeuThr: 5.895 ± 0.414
5.651LeuVal: 5.651 ± 0.545
0.854LeuTrp: 0.854 ± 0.189
2.521LeuTyr: 2.521 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
2.602MetAla: 2.602 ± 0.395
0.285MetCys: 0.285 ± 0.12
1.748MetAsp: 1.748 ± 0.232
1.138MetGlu: 1.138 ± 0.212
0.935MetPhe: 0.935 ± 0.2
1.748MetGly: 1.748 ± 0.242
0.529MetHis: 0.529 ± 0.127
1.992MetIle: 1.992 ± 0.222
1.504MetLys: 1.504 ± 0.223
1.911MetLeu: 1.911 ± 0.307
0.325MetMet: 0.325 ± 0.106
1.098MetAsn: 1.098 ± 0.194
1.016MetPro: 1.016 ± 0.225
0.569MetGln: 0.569 ± 0.13
1.382MetArg: 1.382 ± 0.226
2.236MetSer: 2.236 ± 0.353
2.317MetThr: 2.317 ± 0.33
2.114MetVal: 2.114 ± 0.264
0.447MetTrp: 0.447 ± 0.122
0.813MetTyr: 0.813 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
3.781AsnAla: 3.781 ± 0.397
0.325AsnCys: 0.325 ± 0.136
3.049AsnAsp: 3.049 ± 0.337
2.968AsnGlu: 2.968 ± 0.379
1.626AsnPhe: 1.626 ± 0.324
4.106AsnGly: 4.106 ± 0.316
0.813AsnHis: 0.813 ± 0.179
2.399AsnIle: 2.399 ± 0.273
2.236AsnLys: 2.236 ± 0.308
3.334AsnLeu: 3.334 ± 0.373
1.179AsnMet: 1.179 ± 0.218
2.236AsnAsn: 2.236 ± 0.377
2.114AsnPro: 2.114 ± 0.329
1.423AsnGln: 1.423 ± 0.271
2.643AsnArg: 2.643 ± 0.337
3.456AsnSer: 3.456 ± 0.356
2.48AsnThr: 2.48 ± 0.322
2.724AsnVal: 2.724 ± 0.287
0.651AsnTrp: 0.651 ± 0.149
1.301AsnTyr: 1.301 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
2.927ProAla: 2.927 ± 0.308
0.122ProCys: 0.122 ± 0.065
2.927ProAsp: 2.927 ± 0.331
3.984ProGlu: 3.984 ± 0.55
1.342ProPhe: 1.342 ± 0.205
3.171ProGly: 3.171 ± 0.554
0.894ProHis: 0.894 ± 0.211
1.748ProIle: 1.748 ± 0.241
2.48ProLys: 2.48 ± 0.268
2.195ProLeu: 2.195 ± 0.287
0.976ProMet: 0.976 ± 0.2
1.708ProAsn: 1.708 ± 0.249
1.748ProPro: 1.748 ± 0.361
1.138ProGln: 1.138 ± 0.246
1.87ProArg: 1.87 ± 0.326
3.131ProSer: 3.131 ± 0.425
2.805ProThr: 2.805 ± 0.401
2.561ProVal: 2.561 ± 0.337
0.529ProTrp: 0.529 ± 0.167
1.708ProTyr: 1.708 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
3.171GlnAla: 3.171 ± 0.35
0.244GlnCys: 0.244 ± 0.109
1.992GlnAsp: 1.992 ± 0.305
2.277GlnGlu: 2.277 ± 0.29
1.138GlnPhe: 1.138 ± 0.194
2.114GlnGly: 2.114 ± 0.315
0.651GlnHis: 0.651 ± 0.188
2.074GlnIle: 2.074 ± 0.315
2.155GlnLys: 2.155 ± 0.398
2.968GlnLeu: 2.968 ± 0.49
0.935GlnMet: 0.935 ± 0.209
1.545GlnAsn: 1.545 ± 0.221
0.854GlnPro: 0.854 ± 0.198
0.935GlnGln: 0.935 ± 0.228
2.439GlnArg: 2.439 ± 0.41
1.911GlnSer: 1.911 ± 0.257
2.195GlnThr: 2.195 ± 0.299
1.87GlnVal: 1.87 ± 0.312
0.163GlnTrp: 0.163 ± 0.082
1.342GlnTyr: 1.342 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
4.147ArgAla: 4.147 ± 0.379
0.285ArgCys: 0.285 ± 0.093
3.171ArgAsp: 3.171 ± 0.323
3.984ArgGlu: 3.984 ± 0.687
2.358ArgPhe: 2.358 ± 0.324
3.334ArgGly: 3.334 ± 0.434
1.098ArgHis: 1.098 ± 0.269
3.415ArgIle: 3.415 ± 0.418
3.984ArgLys: 3.984 ± 0.473
4.228ArgLeu: 4.228 ± 0.556
1.545ArgMet: 1.545 ± 0.271
2.439ArgAsn: 2.439 ± 0.414
1.911ArgPro: 1.911 ± 0.353
1.992ArgGln: 1.992 ± 0.269
3.74ArgArg: 3.74 ± 0.454
3.049ArgSer: 3.049 ± 0.357
4.147ArgThr: 4.147 ± 0.377
3.781ArgVal: 3.781 ± 0.47
0.894ArgTrp: 0.894 ± 0.178
2.114ArgTyr: 2.114 ± 0.298
0.0ArgXaa: 0.0 ± 0.0
Ser
5.733SerAla: 5.733 ± 0.617
0.244SerCys: 0.244 ± 0.098
3.781SerAsp: 3.781 ± 0.368
4.025SerGlu: 4.025 ± 0.441
2.236SerPhe: 2.236 ± 0.278
5.855SerGly: 5.855 ± 0.695
1.179SerHis: 1.179 ± 0.239
3.415SerIle: 3.415 ± 0.32
3.293SerLys: 3.293 ± 0.376
5.57SerLeu: 5.57 ± 0.53
1.626SerMet: 1.626 ± 0.173
2.683SerAsn: 2.683 ± 0.342
2.602SerPro: 2.602 ± 0.308
1.87SerGln: 1.87 ± 0.228
2.765SerArg: 2.765 ± 0.378
3.781SerSer: 3.781 ± 0.506
4.35SerThr: 4.35 ± 0.76
5.326SerVal: 5.326 ± 0.572
1.504SerTrp: 1.504 ± 0.228
2.195SerTyr: 2.195 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
6.058ThrAla: 6.058 ± 0.666
0.244ThrCys: 0.244 ± 0.097
3.7ThrAsp: 3.7 ± 0.536
3.903ThrGlu: 3.903 ± 0.426
2.033ThrPhe: 2.033 ± 0.291
5.001ThrGly: 5.001 ± 0.705
1.057ThrHis: 1.057 ± 0.191
4.554ThrIle: 4.554 ± 0.55
4.147ThrLys: 4.147 ± 0.398
4.798ThrLeu: 4.798 ± 0.405
1.586ThrMet: 1.586 ± 0.355
2.805ThrAsn: 2.805 ± 0.488
3.456ThrPro: 3.456 ± 0.422
2.317ThrGln: 2.317 ± 0.278
2.846ThrArg: 2.846 ± 0.306
4.228ThrSer: 4.228 ± 0.435
4.594ThrThr: 4.594 ± 0.732
5.692ThrVal: 5.692 ± 0.75
1.22ThrTrp: 1.22 ± 0.304
2.155ThrTyr: 2.155 ± 0.338
0.0ThrXaa: 0.0 ± 0.0
Val
6.18ValAla: 6.18 ± 0.496
0.325ValCys: 0.325 ± 0.115
4.35ValAsp: 4.35 ± 0.35
5.407ValGlu: 5.407 ± 0.557
2.358ValPhe: 2.358 ± 0.327
4.594ValGly: 4.594 ± 0.477
1.016ValHis: 1.016 ± 0.188
4.513ValIle: 4.513 ± 0.469
3.7ValLys: 3.7 ± 0.401
6.18ValLeu: 6.18 ± 0.512
2.114ValMet: 2.114 ± 0.31
2.927ValAsn: 2.927 ± 0.359
3.049ValPro: 3.049 ± 0.333
1.789ValGln: 1.789 ± 0.262
4.35ValArg: 4.35 ± 0.478
4.432ValSer: 4.432 ± 0.483
5.204ValThr: 5.204 ± 0.544
4.838ValVal: 4.838 ± 0.523
0.813ValTrp: 0.813 ± 0.173
1.911ValTyr: 1.911 ± 0.321
0.0ValXaa: 0.0 ± 0.0
Trp
1.22TrpAla: 1.22 ± 0.192
0.041TrpCys: 0.041 ± 0.044
0.935TrpAsp: 0.935 ± 0.191
0.854TrpGlu: 0.854 ± 0.171
0.488TrpPhe: 0.488 ± 0.132
1.382TrpGly: 1.382 ± 0.266
0.203TrpHis: 0.203 ± 0.098
0.854TrpIle: 0.854 ± 0.197
1.016TrpLys: 1.016 ± 0.203
1.301TrpLeu: 1.301 ± 0.244
0.61TrpMet: 0.61 ± 0.147
0.813TrpAsn: 0.813 ± 0.197
0.569TrpPro: 0.569 ± 0.151
0.569TrpGln: 0.569 ± 0.161
0.976TrpArg: 0.976 ± 0.209
1.26TrpSer: 1.26 ± 0.283
1.179TrpThr: 1.179 ± 0.336
1.016TrpVal: 1.016 ± 0.185
0.366TrpTrp: 0.366 ± 0.136
0.447TrpTyr: 0.447 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.602TyrAla: 2.602 ± 0.35
0.203TyrCys: 0.203 ± 0.081
2.277TyrAsp: 2.277 ± 0.313
2.114TyrGlu: 2.114 ± 0.306
0.976TyrPhe: 0.976 ± 0.183
2.48TyrGly: 2.48 ± 0.397
0.285TyrHis: 0.285 ± 0.117
2.277TyrIle: 2.277 ± 0.337
1.789TyrLys: 1.789 ± 0.282
3.09TyrLeu: 3.09 ± 0.334
0.447TyrMet: 0.447 ± 0.142
1.382TyrAsn: 1.382 ± 0.224
1.545TyrPro: 1.545 ± 0.228
1.138TyrGln: 1.138 ± 0.153
2.236TyrArg: 2.236 ± 0.368
2.317TyrSer: 2.317 ± 0.291
2.602TyrThr: 2.602 ± 0.362
2.033TyrVal: 2.033 ± 0.293
0.691TyrTrp: 0.691 ± 0.15
1.382TyrTyr: 1.382 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 131 proteins (24597 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski