Bogoriella caseilytica
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N2BEL8|A0A3N2BEL8_9MICO Enterochelin esterase family protein OS=Bogoriella caseilytica OX=56055 GN=EDD31_2091 PE=4 SV=1
MM1 pKa = 7.44 AVLIGVLGSMAMAVPVAADD20 pKa = 3.49 TALEE24 pKa = 3.96 PAIEE28 pKa = 4.75 ADD30 pKa = 4.48 SADD33 pKa = 3.74 ADD35 pKa = 3.8 ADD37 pKa = 3.74 ADD39 pKa = 3.97 ADD41 pKa = 3.74 ADD43 pKa = 3.97 AAADD47 pKa = 3.61 AAAEE51 pKa = 4.12 ADD53 pKa = 3.83 STEE56 pKa = 4.02 AAAEE60 pKa = 4.05 EE61 pKa = 4.21 DD62 pKa = 4.37 TEE64 pKa = 4.5 TLSDD68 pKa = 3.62 TADD71 pKa = 3.36 EE72 pKa = 4.54 GTPATSSDD80 pKa = 3.91 DD81 pKa = 3.58 VSTLAEE87 pKa = 3.87 VSPFAEE93 pKa = 4.35 PSPVYY98 pKa = 10.21 EE99 pKa = 4.01 ITGVWLEE106 pKa = 4.04 QPEE109 pKa = 4.55 VTSRR113 pKa = 11.84 GNPVVADD120 pKa = 3.15 WRR122 pKa = 11.84 INVNDD127 pKa = 3.99 GNNPPSNEE135 pKa = 3.78 LVDD138 pKa = 4.2 NVVATFDD145 pKa = 3.19 VGNAVFQTIPDD156 pKa = 3.79 LCLTTGVQPASSISADD172 pKa = 3.59 GSLLTCNFGTVALGRR187 pKa = 11.84 SIALQTPVITEE198 pKa = 4.28 GVTGTNVSLSGTSPAGEE215 pKa = 4.11 TVEE218 pKa = 5.3 LPLIPIQNDD227 pKa = 2.77 FRR229 pKa = 11.84 MDD231 pKa = 3.14 MHH233 pKa = 7.16 FGQNTNQRR241 pKa = 11.84 EE242 pKa = 4.16 WDD244 pKa = 3.79 SLSNPTNVNLDD255 pKa = 3.85 VQWSLRR261 pKa = 11.84 LGKK264 pKa = 10.77 GSDD267 pKa = 3.72 PGPANQTFRR276 pKa = 11.84 LRR278 pKa = 11.84 IAPIFPAGSTVEE290 pKa = 4.34 VGTHH294 pKa = 6.4 IYY296 pKa = 9.35 TGVVGCSPFDD306 pKa = 3.75 LSNAPGHH313 pKa = 6.81 PYY315 pKa = 10.46 SSNPPHH321 pKa = 6.65 PRR323 pKa = 11.84 HH324 pKa = 5.89 TNFVDD329 pKa = 3.41 SCTLTAVPGEE339 pKa = 4.12 PGVFDD344 pKa = 4.03 LTLEE348 pKa = 4.64 GINYY352 pKa = 10.0 SLVNAPTNDD361 pKa = 3.2 AGTANSPLPADD372 pKa = 3.55 WDD374 pKa = 4.28 YY375 pKa = 11.82 VASGSLWFQVNAGSNGSLALTMDD398 pKa = 4.43 PVTYY402 pKa = 9.18 TATTGQTSEE411 pKa = 5.0 DD412 pKa = 3.89 LGTNNQTTKK421 pKa = 11.17 AFTLPGAWSASFRR434 pKa = 11.84 RR435 pKa = 11.84 SATGSGGTFWDD446 pKa = 3.49 DD447 pKa = 3.37 SYY449 pKa = 11.78 RR450 pKa = 11.84 VTPGVTLRR458 pKa = 11.84 NEE460 pKa = 3.75 ARR462 pKa = 11.84 NGYY465 pKa = 10.06 SGDD468 pKa = 3.59 RR469 pKa = 11.84 NVAPTAQYY477 pKa = 10.82 GSCLALDD484 pKa = 3.66 TRR486 pKa = 11.84 YY487 pKa = 10.88 VIVDD491 pKa = 3.56 RR492 pKa = 11.84 DD493 pKa = 3.23 FDD495 pKa = 4.23 LAVATGFLARR505 pKa = 11.84 NDD507 pKa = 3.62 WPASAVLEE515 pKa = 4.44 YY516 pKa = 10.92 YY517 pKa = 10.88 VGGSTLLDD525 pKa = 3.87 PATGNTAYY533 pKa = 10.87 NPDD536 pKa = 3.55 QFEE539 pKa = 4.54 CDD541 pKa = 3.66 SDD543 pKa = 3.84 PGGWTTEE550 pKa = 4.05 LPADD554 pKa = 4.38 LSTIRR559 pKa = 11.84 AARR562 pKa = 11.84 VTYY565 pKa = 10.27 DD566 pKa = 3.48 FSSFDD571 pKa = 3.33 EE572 pKa = 4.35 VGRR575 pKa = 11.84 EE576 pKa = 4.09 FIDD579 pKa = 3.65 LVGWTTVRR587 pKa = 11.84 EE588 pKa = 4.05 DD589 pKa = 3.49 VQPGQDD595 pKa = 2.07 IWTFGSRR602 pKa = 11.84 LRR604 pKa = 11.84 NGGWFGPAQEE614 pKa = 4.57 EE615 pKa = 5.39 VITPTPDD622 pKa = 2.52 ARR624 pKa = 11.84 YY625 pKa = 8.07 PHH627 pKa = 6.65 TDD629 pKa = 2.44 GRR631 pKa = 11.84 RR632 pKa = 11.84 DD633 pKa = 3.2 IARR636 pKa = 11.84 VISANPAIQKK646 pKa = 9.26 SAEE649 pKa = 3.98 RR650 pKa = 11.84 SVVVPGVPTVFTLRR664 pKa = 11.84 YY665 pKa = 8.55 SANTGAGIPEE675 pKa = 4.35 SVDD678 pKa = 2.96 GYY680 pKa = 9.1 TVVDD684 pKa = 3.81 TLPPLVTYY692 pKa = 9.71 EE693 pKa = 4.28 VGSSDD698 pKa = 4.18 PEE700 pKa = 4.05 PTVTTNANNQQVLTWTFDD718 pKa = 3.57 NVPTNVQNVLTYY730 pKa = 10.73 AAAADD735 pKa = 3.9 SSVAPGTPLVNTATASLGGTTSSPARR761 pKa = 11.84 ATVTTANNGFTTILKK776 pKa = 9.07 TSDD779 pKa = 3.1 VEE781 pKa = 4.59 FIPNRR786 pKa = 11.84 LGDD789 pKa = 4.08 GVGSGSWTVTIEE801 pKa = 4.03 SRR803 pKa = 11.84 DD804 pKa = 3.85 PQSQAWTDD812 pKa = 3.68 TIDD815 pKa = 3.21 ILPYY819 pKa = 10.53 NGDD822 pKa = 3.13 GRR824 pKa = 11.84 GTSFSGTYY832 pKa = 9.37 TLGDD836 pKa = 3.27 VVLFDD841 pKa = 5.17 GGTLYY846 pKa = 9.82 YY847 pKa = 10.43 TDD849 pKa = 5.36 ADD851 pKa = 4.23 PATLSDD857 pKa = 4.56 DD858 pKa = 4.08 PAHH861 pKa = 6.79 EE862 pKa = 4.96 SNGAPNAPSALWSTEE877 pKa = 3.66 RR878 pKa = 11.84 PEE880 pKa = 4.12 NPTAIRR886 pKa = 11.84 VIGGTLATGGSFSFQVPITTDD907 pKa = 3.02 GAEE910 pKa = 4.15 AQDD913 pKa = 3.61 VFVNRR918 pKa = 11.84 AQARR922 pKa = 11.84 AEE924 pKa = 3.91 HH925 pKa = 6.5 TEE927 pKa = 3.72 LVMRR931 pKa = 11.84 TSAALVVTDD940 pKa = 3.55 YY941 pKa = 11.32 LVEE944 pKa = 4.24 KK945 pKa = 10.22 SANPEE950 pKa = 3.7 SGSTVRR956 pKa = 11.84 PGDD959 pKa = 3.58 VVEE962 pKa = 4.26 YY963 pKa = 9.29 TVTVSQMGDD972 pKa = 3.17 VPAGAWFSDD981 pKa = 3.68 DD982 pKa = 3.71 MSDD985 pKa = 4.18 VLDD988 pKa = 4.42 DD989 pKa = 4.02 ADD991 pKa = 3.99 YY992 pKa = 11.51 NGDD995 pKa = 3.17 VTASHH1000 pKa = 6.79 GEE1002 pKa = 3.97 VLSFEE1007 pKa = 4.28 NGILEE1012 pKa = 4.37 WEE1014 pKa = 4.6 GVVPVGEE1021 pKa = 4.35 IATITYY1027 pKa = 10.29 SVTVKK1032 pKa = 10.44 AAEE1035 pKa = 4.09 EE1036 pKa = 3.85 LRR1038 pKa = 11.84 EE1039 pKa = 4.13 GDD1041 pKa = 4.7 SNTFLNNQVTSPGCQGACSTEE1062 pKa = 4.02 HH1063 pKa = 6.03 LVGYY1067 pKa = 9.57 YY1068 pKa = 9.96 SVSKK1072 pKa = 9.09 TAEE1075 pKa = 4.03 PAPGEE1080 pKa = 4.19 TVRR1083 pKa = 11.84 IGDD1086 pKa = 3.87 VITYY1090 pKa = 8.78 HH1091 pKa = 5.79 VQVTQHH1097 pKa = 6.42 GEE1099 pKa = 4.1 GAVDD1103 pKa = 3.2 NAFFIDD1109 pKa = 4.52 DD1110 pKa = 4.15 LSEE1113 pKa = 4.15 VLDD1116 pKa = 3.96 DD1117 pKa = 6.57 AIWNDD1122 pKa = 3.64 DD1123 pKa = 3.32 AAATDD1128 pKa = 4.39 GEE1130 pKa = 4.57 LSFDD1134 pKa = 3.88 EE1135 pKa = 6.13 PILSWSAPLEE1145 pKa = 4.32 VGDD1148 pKa = 4.39 VVDD1151 pKa = 3.37 VTYY1154 pKa = 11.08 SVTVTGEE1161 pKa = 3.49 GDD1163 pKa = 3.5 YY1164 pKa = 11.05 EE1165 pKa = 4.1 LRR1167 pKa = 11.84 NVVSSVCAEE1176 pKa = 4.62 DD1177 pKa = 6.06 DD1178 pKa = 4.05 PDD1180 pKa = 4.21 CLEE1183 pKa = 5.05 AICVPAADD1191 pKa = 4.8 EE1192 pKa = 5.12 NPDD1195 pKa = 3.53 CTTEE1199 pKa = 4.58 HH1200 pKa = 6.59 IVGDD1204 pKa = 3.86 YY1205 pKa = 10.63 QVTKK1209 pKa = 10.9 VSDD1212 pKa = 4.07 PEE1214 pKa = 4.14 SGSAVEE1220 pKa = 4.34 VGDD1223 pKa = 4.0 VIDD1226 pKa = 3.76 YY1227 pKa = 7.79 TVTVSHH1233 pKa = 7.04 IGLADD1238 pKa = 3.56 VEE1240 pKa = 4.41 ASFVDD1245 pKa = 3.97 DD1246 pKa = 5.32 LSDD1249 pKa = 4.22 VLDD1252 pKa = 4.09 DD1253 pKa = 6.15 AIWNDD1258 pKa = 4.05 DD1259 pKa = 3.28 VSASAGEE1266 pKa = 4.04 FSFEE1270 pKa = 4.67 GEE1272 pKa = 4.21 TLSWWGALSEE1282 pKa = 4.31 GDD1284 pKa = 4.0 VVTVTYY1290 pKa = 10.65 SVTVTAEE1297 pKa = 3.63 GDD1299 pKa = 3.38 RR1300 pKa = 11.84 YY1301 pKa = 10.78 LEE1303 pKa = 4.16 NVVTTDD1309 pKa = 3.34 GWCVPAEE1316 pKa = 4.39 GQDD1319 pKa = 3.57 EE1320 pKa = 4.54 ACRR1323 pKa = 11.84 TVHH1326 pKa = 6.75 INGAYY1331 pKa = 8.38 TYY1333 pKa = 9.31 SKK1335 pKa = 10.66 SSEE1338 pKa = 4.31 SPSGSLVRR1346 pKa = 11.84 PGEE1349 pKa = 4.27 VVTYY1353 pKa = 8.17 TLEE1356 pKa = 4.12 VEE1358 pKa = 4.77 HH1359 pKa = 6.94 VGTAPVSGAIVTDD1372 pKa = 4.36 DD1373 pKa = 4.02 LSQVLPYY1380 pKa = 10.94 ASWNDD1385 pKa = 3.47 DD1386 pKa = 3.39 AEE1388 pKa = 4.53 ATDD1391 pKa = 3.81 GTVVRR1396 pKa = 11.84 DD1397 pKa = 3.9 GEE1399 pKa = 4.54 TLTWTGDD1406 pKa = 3.66 LAVGQVVEE1414 pKa = 4.11 ITYY1417 pKa = 10.68 SVTVGDD1423 pKa = 4.23 LEE1425 pKa = 4.59 EE1426 pKa = 4.46 IAMVNVVTSPDD1437 pKa = 3.21 EE1438 pKa = 4.03 RR1439 pKa = 11.84 AVCVPAADD1447 pKa = 4.25 EE1448 pKa = 4.46 NADD1451 pKa = 3.8 CTTEE1455 pKa = 4.21 HH1456 pKa = 6.72 YY1457 pKa = 10.78 NGAYY1461 pKa = 8.79 TFSKK1465 pKa = 9.9 TSDD1468 pKa = 3.62 PASGSVVEE1476 pKa = 4.47 AGEE1479 pKa = 4.34 VVTYY1483 pKa = 8.45 TVVVEE1488 pKa = 4.53 HH1489 pKa = 6.74 YY1490 pKa = 8.08 GTAGVQDD1497 pKa = 5.23 AIVVDD1502 pKa = 4.45 DD1503 pKa = 4.0 LTGVLAYY1510 pKa = 10.43 AEE1512 pKa = 4.4 WNDD1515 pKa = 4.28 DD1516 pKa = 3.57 AQADD1520 pKa = 4.11 SGDD1523 pKa = 3.53 LAYY1526 pKa = 10.37 ADD1528 pKa = 5.01 EE1529 pKa = 4.34 EE1530 pKa = 4.7 LVWSGDD1536 pKa = 3.54 LAIGQVVEE1544 pKa = 3.84 ITYY1547 pKa = 10.64 SVTVGDD1553 pKa = 3.97 EE1554 pKa = 4.3 PEE1556 pKa = 4.12 ATLHH1560 pKa = 6.12 NVVTSPDD1567 pKa = 3.45 DD1568 pKa = 3.46 RR1569 pKa = 11.84 AVCVPAADD1577 pKa = 4.28 GNAGCATEE1585 pKa = 4.64 HH1586 pKa = 5.53 TTPPDD1591 pKa = 3.56 APGKK1595 pKa = 9.45 PPVPGLPSTGALLPWGMGLVALLLIACGGVLTITEE1630 pKa = 3.93 QRR1632 pKa = 11.84 KK1633 pKa = 7.82 AASRR1637 pKa = 11.84 ARR1639 pKa = 11.84 QQIGTDD1645 pKa = 3.12 RR1646 pKa = 11.84 FF1647 pKa = 3.63
Molecular weight: 173.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.897
Patrickios 1.685
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A3N2BG53|A0A3N2BG53_9MICO 23S rRNA (Cytidine1920-2'-O)/16S rRNA (Cytidine1409-2'-O)-methyltransferase OS=Bogoriella caseilytica OX=56055 GN=EDD31_2631 PE=4 SV=1
MM1 pKa = 7.3 ARR3 pKa = 11.84 AARR6 pKa = 11.84 GNIVLALLALLALLALLALLALLALLALLALARR39 pKa = 11.84 PAGTSRR45 pKa = 11.84 LGAATSGPTRR55 pKa = 11.84 RR56 pKa = 11.84 ATPHH60 pKa = 6.69 RR61 pKa = 11.84 LPRR64 pKa = 11.84 DD65 pKa = 3.37 AGGVRR70 pKa = 3.61
Molecular weight: 7.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.774
IPC_protein 12.384
Toseland 12.544
ProMoST 13.056
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.047
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.798
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2844
0
2844
992085
30
2576
348.8
37.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.521 ± 0.061
0.539 ± 0.011
5.789 ± 0.043
6.728 ± 0.051
2.756 ± 0.03
9.151 ± 0.041
2.257 ± 0.024
3.916 ± 0.028
1.493 ± 0.03
10.372 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.922 ± 0.019
1.78 ± 0.022
5.721 ± 0.04
3.088 ± 0.024
7.372 ± 0.058
5.59 ± 0.029
5.897 ± 0.038
8.62 ± 0.043
1.539 ± 0.019
1.949 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here