Gordonia phage Coeur
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6EFD0|A0A4Y6EFD0_9CAUD Immunity repressor OS=Gordonia phage Coeur OX=2571246 GN=20 PE=4 SV=1
MM1 pKa = 7.94 AIRR4 pKa = 11.84 RR5 pKa = 11.84 PPLALGPGSLVFGAVGSQLDD25 pKa = 3.58 ISCQVTAAKK34 pKa = 9.1 VTFDD38 pKa = 4.01 NDD40 pKa = 4.02 KK41 pKa = 11.28 EE42 pKa = 4.44 DD43 pKa = 5.67 DD44 pKa = 4.17 LPTLCGSTIVGEE56 pKa = 4.32 KK57 pKa = 10.36 SYY59 pKa = 9.16 TAQLEE64 pKa = 4.11 FSAAQDD70 pKa = 3.34 IEE72 pKa = 4.64 ADD74 pKa = 4.43 GLIDD78 pKa = 3.81 WTWKK82 pKa = 9.77 NAGAEE87 pKa = 4.47 VPFSFIPKK95 pKa = 8.82 EE96 pKa = 4.16 GEE98 pKa = 3.79 TAAVTGVVVVDD109 pKa = 3.75 PVEE112 pKa = 4.1 FGGDD116 pKa = 3.27 VKK118 pKa = 10.85 KK119 pKa = 11.03 RR120 pKa = 11.84 NISDD124 pKa = 3.91 AEE126 pKa = 4.11 WNIVGMPAFTPDD138 pKa = 2.87 STADD142 pKa = 3.4 STEE145 pKa = 3.91 YY146 pKa = 10.47 PPLEE150 pKa = 4.49 DD151 pKa = 3.72
Molecular weight: 16.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.296
IPC2_protein 3.961
IPC_protein 3.923
Toseland 3.719
ProMoST 4.024
Dawson 3.897
Bjellqvist 4.101
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.63
Solomon 3.897
Lehninger 3.846
Nozaki 4.024
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 3.77
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.984
Protein with the highest isoelectric point:
>tr|A0A4Y6EJ92|A0A4Y6EJ92_9CAUD Uncharacterized protein OS=Gordonia phage Coeur OX=2571246 GN=13 PE=4 SV=1
MM1 pKa = 7.16 ARR3 pKa = 11.84 KK4 pKa = 8.34 YY5 pKa = 9.43 TIDD8 pKa = 3.55 STPWSAVVVCNRR20 pKa = 11.84 RR21 pKa = 11.84 GCFWRR26 pKa = 11.84 GFAHH30 pKa = 6.55 TKK32 pKa = 8.61 PSAYY36 pKa = 10.13 RR37 pKa = 11.84 LVADD41 pKa = 4.2 HH42 pKa = 7.04 LRR44 pKa = 11.84 RR45 pKa = 11.84 AHH47 pKa = 7.29 DD48 pKa = 4.08 DD49 pKa = 3.07 AAAADD54 pKa = 4.49 DD55 pKa = 4.4 ARR57 pKa = 11.84 QSAQRR62 pKa = 11.84 HH63 pKa = 4.58 NKK65 pKa = 7.08 TFRR68 pKa = 11.84 NQRR71 pKa = 11.84 APGGKK76 pKa = 9.04 SAAA79 pKa = 3.9
Molecular weight: 8.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.78
IPC_protein 10.862
Toseland 10.994
ProMoST 11.023
Dawson 11.038
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.082
Grimsley 11.082
Solomon 11.316
Lehninger 11.257
Nozaki 10.979
DTASelect 10.862
Thurlkill 10.979
EMBOSS 11.433
Sillero 11.008
Patrickios 10.862
IPC_peptide 11.316
IPC2_peptide 10.189
IPC2.peptide.svr19 8.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26
0
26
5130
35
683
197.3
21.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.893 ± 1.013
0.819 ± 0.202
6.335 ± 0.651
4.191 ± 0.434
2.573 ± 0.196
8.402 ± 0.488
2.222 ± 0.343
4.932 ± 0.5
1.618 ± 0.195
7.875 ± 0.241
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.105 ± 0.171
3.021 ± 0.369
6.199 ± 0.38
2.573 ± 0.235
7.544 ± 0.722
5.361 ± 0.356
7.29 ± 0.417
7.563 ± 0.468
2.359 ± 0.29
2.125 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here