Tobacco rattle virus (isolate PpK20) (TRV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobravirus; Tobacco rattle virus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q88898|P40_TRVPP 40 kDa protein OS=Tobacco rattle virus (isolate PpK20) OX=652939 PE=1 SV=2
MM1 pKa = 7.67GDD3 pKa = 3.41MYY5 pKa = 11.37DD6 pKa = 3.43EE7 pKa = 5.12SFDD10 pKa = 4.02KK11 pKa = 11.31SGGPADD17 pKa = 5.15LMDD20 pKa = 5.27DD21 pKa = 3.49SWVEE25 pKa = 4.09SVSWKK30 pKa = 10.78DD31 pKa = 3.43LLKK34 pKa = 10.54KK35 pKa = 10.22LHH37 pKa = 6.53SIKK40 pKa = 10.42FALQSGRR47 pKa = 11.84DD48 pKa = 3.84EE49 pKa = 4.16ITGLLAALNRR59 pKa = 11.84QCPYY63 pKa = 10.73SPYY66 pKa = 10.69EE67 pKa = 3.74QFPDD71 pKa = 3.18KK72 pKa = 10.59KK73 pKa = 11.07VYY75 pKa = 10.37FLLDD79 pKa = 3.24SRR81 pKa = 11.84ANSALGVIQNASAFKK96 pKa = 10.58RR97 pKa = 11.84RR98 pKa = 11.84ADD100 pKa = 3.67EE101 pKa = 4.25KK102 pKa = 10.79NAVAGVTNIPANPNTTVTTNQGSTTTTKK130 pKa = 11.09ANTGSTLEE138 pKa = 4.09EE139 pKa = 4.3DD140 pKa = 4.03LYY142 pKa = 11.05TYY144 pKa = 11.21YY145 pKa = 11.11KK146 pKa = 10.5FDD148 pKa = 4.16DD149 pKa = 4.18ASTAFHH155 pKa = 7.14KK156 pKa = 10.98SLTSLEE162 pKa = 3.89NMEE165 pKa = 4.34LKK167 pKa = 10.36SYY169 pKa = 9.62YY170 pKa = 9.67RR171 pKa = 11.84RR172 pKa = 11.84NFEE175 pKa = 3.98KK176 pKa = 10.94VFGIKK181 pKa = 10.35FGGAAASSSAPPPASGGPIRR201 pKa = 11.84PNPP204 pKa = 3.51

Molecular weight: 22.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q88897|CAPSD_TRVPP Capsid protein OS=Tobacco rattle virus (isolate PpK20) OX=652939 GN=CP PE=1 SV=1
MM1 pKa = 7.04TCVLKK6 pKa = 11.2GCVNEE11 pKa = 4.24VTVLGHH17 pKa = 5.19EE18 pKa = 4.44TCSIGHH24 pKa = 6.29ANKK27 pKa = 9.76LRR29 pKa = 11.84KK30 pKa = 9.02QVADD34 pKa = 3.37MVGVTRR40 pKa = 11.84RR41 pKa = 11.84CAEE44 pKa = 4.07NNCGWFVCVVINDD57 pKa = 3.74FTFDD61 pKa = 3.52VYY63 pKa = 11.0NCCGRR68 pKa = 11.84SHH70 pKa = 7.34LEE72 pKa = 3.56KK73 pKa = 10.29CRR75 pKa = 11.84KK76 pKa = 9.4RR77 pKa = 11.84VEE79 pKa = 4.05TRR81 pKa = 11.84NRR83 pKa = 11.84EE84 pKa = 3.06IWKK87 pKa = 9.7QIRR90 pKa = 11.84RR91 pKa = 11.84NQAEE95 pKa = 4.34NMSATAKK102 pKa = 10.16KK103 pKa = 9.75SHH105 pKa = 6.18NSKK108 pKa = 9.11TSKK111 pKa = 10.63KK112 pKa = 9.81KK113 pKa = 10.14FKK115 pKa = 10.11EE116 pKa = 3.71DD117 pKa = 3.85RR118 pKa = 11.84EE119 pKa = 4.15FGTPKK124 pKa = 10.35RR125 pKa = 11.84FLRR128 pKa = 11.84DD129 pKa = 3.62DD130 pKa = 3.66VPFGIDD136 pKa = 2.78RR137 pKa = 11.84LFAFF141 pKa = 5.32

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2950

141

1707

491.7

55.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.068 ± 0.604

1.763 ± 0.441

6.915 ± 0.27

5.864 ± 0.244

4.644 ± 0.207

5.898 ± 0.775

1.932 ± 0.244

4.271 ± 0.236

7.492 ± 0.586

8.983 ± 0.747

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.441 ± 0.342

4.407 ± 0.371

3.492 ± 0.427

3.288 ± 0.326

5.898 ± 0.417

7.559 ± 0.664

5.017 ± 0.443

8.746 ± 0.689

1.356 ± 0.106

3.932 ± 0.583
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski