Human papillomavirus type 5b

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Zurhausenvirales; Papillomaviridae; Firstpapillomavirinae; Betapapillomavirus; Betapapillomavirus 1

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P26559|VE7_HPV5B Protein E7 OS=Human papillomavirus type 5b OX=10599 GN=E7 PE=3 SV=1
MM1 pKa = 7.41IGKK4 pKa = 8.9EE5 pKa = 4.0VTVQDD10 pKa = 4.38IILEE14 pKa = 4.05LSEE17 pKa = 4.23VQPEE21 pKa = 4.28VLPVDD26 pKa = 4.17LFCEE30 pKa = 4.15EE31 pKa = 4.45EE32 pKa = 4.38LPNEE36 pKa = 4.16QEE38 pKa = 4.26TEE40 pKa = 4.09EE41 pKa = 4.39EE42 pKa = 3.92PDD44 pKa = 3.26IEE46 pKa = 4.74RR47 pKa = 11.84ISYY50 pKa = 9.99KK51 pKa = 10.66VIAPCGCRR59 pKa = 11.84HH60 pKa = 6.38CEE62 pKa = 3.5VKK64 pKa = 10.77LRR66 pKa = 11.84IFVHH70 pKa = 5.08ATEE73 pKa = 4.28FGIRR77 pKa = 11.84AFQQLLTGDD86 pKa = 4.25LQLLCPDD93 pKa = 3.44CRR95 pKa = 11.84GNCKK99 pKa = 9.94HH100 pKa = 7.02DD101 pKa = 3.47GSS103 pKa = 4.21

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P26550|VE4_HPV5B Protein E4 OS=Human papillomavirus type 5b OX=10599 GN=E4 PE=3 SV=2
MM1 pKa = 7.68EE2 pKa = 5.02NLSEE6 pKa = 4.76RR7 pKa = 11.84FNALQDD13 pKa = 3.48QLMNIYY19 pKa = 9.48EE20 pKa = 4.27AAEE23 pKa = 3.98QTLQAQIKK31 pKa = 8.71HH32 pKa = 4.61WQTLRR37 pKa = 11.84KK38 pKa = 9.06EE39 pKa = 4.28AVLLYY44 pKa = 8.61YY45 pKa = 10.61ARR47 pKa = 11.84EE48 pKa = 4.03KK49 pKa = 11.0GVTRR53 pKa = 11.84LGYY56 pKa = 10.26QPVPVKK62 pKa = 10.56AVSEE66 pKa = 4.56TKK68 pKa = 10.5AKK70 pKa = 10.15EE71 pKa = 4.07AIAMVLQLEE80 pKa = 4.42SLQTSDD86 pKa = 4.56FAHH89 pKa = 6.46EE90 pKa = 4.1PWTLVDD96 pKa = 3.67TSTEE100 pKa = 4.25TFRR103 pKa = 11.84SAPEE107 pKa = 3.64GHH109 pKa = 6.37FKK111 pKa = 10.66KK112 pKa = 10.8GPVPVEE118 pKa = 3.74VIYY121 pKa = 11.25DD122 pKa = 3.85NDD124 pKa = 3.86PDD126 pKa = 3.89NANLYY131 pKa = 8.02TMWTYY136 pKa = 11.13VYY138 pKa = 11.46YY139 pKa = 10.27MDD141 pKa = 6.38ADD143 pKa = 4.42DD144 pKa = 4.48KK145 pKa = 9.72WHH147 pKa = 6.99KK148 pKa = 10.57ARR150 pKa = 11.84SGVNHH155 pKa = 6.5IGIYY159 pKa = 9.54YY160 pKa = 9.95LQGTFKK166 pKa = 10.86NYY168 pKa = 10.12YY169 pKa = 10.21VLFADD174 pKa = 3.83DD175 pKa = 3.8AKK177 pKa = 10.94RR178 pKa = 11.84YY179 pKa = 6.43GTTGEE184 pKa = 4.16WEE186 pKa = 4.22VKK188 pKa = 9.71VNKK191 pKa = 9.12DD192 pKa = 3.24TVFAPVTSSTPPGSPGRR209 pKa = 11.84QADD212 pKa = 3.55TDD214 pKa = 4.05TTAKK218 pKa = 10.05TPTTSTTAVDD228 pKa = 3.52STSRR232 pKa = 11.84QLTTSKK238 pKa = 10.35QPQQTEE244 pKa = 4.12TRR246 pKa = 11.84GRR248 pKa = 11.84RR249 pKa = 11.84YY250 pKa = 10.09GRR252 pKa = 11.84RR253 pKa = 11.84PSSKK257 pKa = 9.79SRR259 pKa = 11.84RR260 pKa = 11.84SQTQQRR266 pKa = 11.84RR267 pKa = 11.84SRR269 pKa = 11.84SRR271 pKa = 11.84HH272 pKa = 4.65RR273 pKa = 11.84SRR275 pKa = 11.84SRR277 pKa = 11.84SRR279 pKa = 11.84SRR281 pKa = 11.84SKK283 pKa = 10.9SQTHH287 pKa = 5.19TTWSTTRR294 pKa = 11.84SRR296 pKa = 11.84STSVGKK302 pKa = 8.3TRR304 pKa = 11.84ALTSRR309 pKa = 11.84SRR311 pKa = 11.84SRR313 pKa = 11.84GRR315 pKa = 11.84SPSTCRR321 pKa = 11.84RR322 pKa = 11.84GGGRR326 pKa = 11.84SPRR329 pKa = 11.84RR330 pKa = 11.84RR331 pKa = 11.84SRR333 pKa = 11.84SPSTYY338 pKa = 10.42SSCTTQRR345 pKa = 11.84SQRR348 pKa = 11.84ARR350 pKa = 11.84AEE352 pKa = 4.19SPTTRR357 pKa = 11.84GARR360 pKa = 11.84GSRR363 pKa = 11.84GSRR366 pKa = 11.84GGSRR370 pKa = 11.84GGRR373 pKa = 11.84LRR375 pKa = 11.84RR376 pKa = 11.84RR377 pKa = 11.84GRR379 pKa = 11.84SSSSSSPAHH388 pKa = 5.78KK389 pKa = 10.08RR390 pKa = 11.84SRR392 pKa = 11.84GGSAKK397 pKa = 10.31LRR399 pKa = 11.84GVSPGEE405 pKa = 3.81VGGSLRR411 pKa = 11.84SVSSKK416 pKa = 9.03HH417 pKa = 4.63TGRR420 pKa = 11.84LGRR423 pKa = 11.84LLEE426 pKa = 4.17EE427 pKa = 4.57ARR429 pKa = 11.84DD430 pKa = 3.72PPVIIVKK437 pKa = 10.23GAANTLKK444 pKa = 10.56YY445 pKa = 10.26FRR447 pKa = 11.84NRR449 pKa = 11.84AKK451 pKa = 10.21IKK453 pKa = 8.54YY454 pKa = 7.56TGLFRR459 pKa = 11.84SFSTTWSWVAGDD471 pKa = 3.34GTEE474 pKa = 3.79RR475 pKa = 11.84LGRR478 pKa = 11.84PRR480 pKa = 11.84MLISFSSYY488 pKa = 7.26SQRR491 pKa = 11.84RR492 pKa = 11.84DD493 pKa = 2.62FDD495 pKa = 3.58EE496 pKa = 4.1AVRR499 pKa = 11.84YY500 pKa = 8.12PKK502 pKa = 10.76GVDD505 pKa = 2.9KK506 pKa = 11.05AYY508 pKa = 10.91GNLDD512 pKa = 3.48SLL514 pKa = 4.64

Molecular weight: 57.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

2941

103

606

326.8

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.712 ± 0.432

1.904 ± 0.604

5.712 ± 0.615

5.916 ± 0.687

3.706 ± 0.605

7.31 ± 0.851

2.89 ± 0.489

4.556 ± 0.736

5.236 ± 0.684

7.718 ± 0.688

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.36 ± 0.361

3.876 ± 0.62

6.936 ± 1.551

5.168 ± 0.387

6.936 ± 1.325

7.446 ± 1.212

6.834 ± 1.014

6.188 ± 0.9

1.292 ± 0.298

3.298 ± 0.445
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski