Pseudomonas phage JBD69

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus; unclassified Casadabanvirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125RND9|A0A125RND9_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD69 OX=1777053 GN=JBD69_52 PE=4 SV=1
MM1 pKa = 7.46SFNSRR6 pKa = 11.84EE7 pKa = 3.86SSLADD12 pKa = 3.51GQPVRR17 pKa = 11.84LYY19 pKa = 10.17QFSRR23 pKa = 11.84GAIRR27 pKa = 11.84WSYY30 pKa = 10.82NSSDD34 pKa = 4.7RR35 pKa = 11.84DD36 pKa = 3.12ITYY39 pKa = 10.21QNQIFRR45 pKa = 11.84TVPGGITDD53 pKa = 3.54NGIICSGDD61 pKa = 3.43PQSDD65 pKa = 3.53QFVITAPADD74 pKa = 3.6LDD76 pKa = 3.68VALLYY81 pKa = 10.5KK82 pKa = 10.44SRR84 pKa = 11.84SPSGAIDD91 pKa = 3.52LVVYY95 pKa = 10.5DD96 pKa = 3.72MHH98 pKa = 8.34YY99 pKa = 11.14GDD101 pKa = 4.46TEE103 pKa = 4.2AAVSWVGQIGDD114 pKa = 4.0VDD116 pKa = 3.86WPTVDD121 pKa = 3.06SCRR124 pKa = 11.84ITCVSEE130 pKa = 4.47DD131 pKa = 3.9EE132 pKa = 6.08LMDD135 pKa = 3.76QPGLIDD141 pKa = 3.86TYY143 pKa = 11.23CRR145 pKa = 11.84TCTAVVGDD153 pKa = 4.48HH154 pKa = 6.1RR155 pKa = 11.84CKK157 pKa = 11.04VNLVPYY163 pKa = 9.97RR164 pKa = 11.84VTLTPQSISGWVISSGVVAGYY185 pKa = 11.08ADD187 pKa = 3.46GWFTGGYY194 pKa = 8.64VEE196 pKa = 4.47WQGDD200 pKa = 3.28GDD202 pKa = 4.34NYY204 pKa = 10.62DD205 pKa = 2.74SRR207 pKa = 11.84YY208 pKa = 9.2IEE210 pKa = 4.13RR211 pKa = 11.84HH212 pKa = 5.85AGPDD216 pKa = 3.48LYY218 pKa = 10.62ILGGTEE224 pKa = 5.08GIPAGGQLRR233 pKa = 11.84VYY235 pKa = 9.42PGCDD239 pKa = 3.1GLAQTCDD246 pKa = 3.63DD247 pKa = 4.57KK248 pKa = 11.59FSNLPNFRR256 pKa = 11.84GFNAMQGKK264 pKa = 9.57SPFDD268 pKa = 4.06GDD270 pKa = 4.13QVWW273 pKa = 3.01

Molecular weight: 29.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125RNB3|A0A125RNB3_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD69 OX=1777053 GN=JBD69_26 PE=4 SV=1
MM1 pKa = 7.71ALQPRR6 pKa = 11.84GIRR9 pKa = 11.84NNNPGNIVWSARR21 pKa = 11.84NNWQGQLPHH30 pKa = 6.42NPKK33 pKa = 9.79VEE35 pKa = 3.88PRR37 pKa = 11.84FARR40 pKa = 11.84FDD42 pKa = 3.52TAHH45 pKa = 6.2NGIRR49 pKa = 11.84ALAKK53 pKa = 10.57LLLNYY58 pKa = 10.24RR59 pKa = 11.84KK60 pKa = 9.47VHH62 pKa = 5.43GLRR65 pKa = 11.84TVEE68 pKa = 3.93SLIARR73 pKa = 11.84WAPSNEE79 pKa = 3.72NDD81 pKa = 2.89TRR83 pKa = 11.84AYY85 pKa = 8.05ATAVARR91 pKa = 11.84AMGVPPQAGLHH102 pKa = 5.82MDD104 pKa = 3.53QDD106 pKa = 4.01TLAALVTAIIRR117 pKa = 11.84HH118 pKa = 5.57EE119 pKa = 4.47NGQQPYY125 pKa = 9.68SAEE128 pKa = 3.99QIAQAVRR135 pKa = 11.84EE136 pKa = 4.2VLL138 pKa = 3.49

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11859

37

1158

208.1

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.623 ± 0.9

0.902 ± 0.153

5.759 ± 0.236

6.088 ± 0.284

2.749 ± 0.231

7.598 ± 0.323

1.729 ± 0.205

4.343 ± 0.198

3.331 ± 0.23

9.849 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.148

2.951 ± 0.245

5.093 ± 0.401

4.857 ± 0.277

7.564 ± 0.378

5.835 ± 0.293

5.616 ± 0.277

6.468 ± 0.264

1.729 ± 0.151

2.682 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski