Amino acid dipepetide frequency for Weissella phage WCP30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.014AlaAla: 8.014 ± 1.745
0.452AlaCys: 0.452 ± 0.276
5.644AlaAsp: 5.644 ± 0.653
4.628AlaGlu: 4.628 ± 1.353
3.386AlaPhe: 3.386 ± 0.606
7.337AlaGly: 7.337 ± 1.218
1.242AlaHis: 1.242 ± 0.409
5.531AlaIle: 5.531 ± 1.055
4.628AlaLys: 4.628 ± 0.729
5.418AlaLeu: 5.418 ± 0.806
2.596AlaMet: 2.596 ± 0.672
6.547AlaAsn: 6.547 ± 1.039
1.58AlaPro: 1.58 ± 0.372
3.725AlaGln: 3.725 ± 0.568
2.935AlaArg: 2.935 ± 0.565
5.192AlaSer: 5.192 ± 0.746
4.289AlaThr: 4.289 ± 0.826
5.418AlaVal: 5.418 ± 0.79
2.37AlaTrp: 2.37 ± 0.599
4.064AlaTyr: 4.064 ± 0.858
0.0AlaXaa: 0.0 ± 0.0
Cys
0.226CysAla: 0.226 ± 0.23
0.0CysCys: 0.0 ± 0.0
0.339CysAsp: 0.339 ± 0.195
0.113CysGlu: 0.113 ± 0.125
0.226CysPhe: 0.226 ± 0.177
0.113CysGly: 0.113 ± 0.103
0.113CysHis: 0.113 ± 0.141
0.113CysIle: 0.113 ± 0.111
0.0CysLys: 0.0 ± 0.0
0.226CysLeu: 0.226 ± 0.186
0.0CysMet: 0.0 ± 0.0
0.113CysAsn: 0.113 ± 0.141
0.113CysPro: 0.113 ± 0.115
0.0CysGln: 0.0 ± 0.0
0.226CysArg: 0.226 ± 0.251
0.564CysSer: 0.564 ± 0.38
0.0CysThr: 0.0 ± 0.0
0.226CysVal: 0.226 ± 0.161
0.113CysTrp: 0.113 ± 0.125
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.757AspAla: 5.757 ± 0.789
0.0AspCys: 0.0 ± 0.0
3.838AspAsp: 3.838 ± 0.855
4.628AspGlu: 4.628 ± 1.047
2.709AspPhe: 2.709 ± 0.72
5.531AspGly: 5.531 ± 0.877
0.452AspHis: 0.452 ± 0.293
4.177AspIle: 4.177 ± 0.689
3.951AspLys: 3.951 ± 0.617
4.289AspLeu: 4.289 ± 0.589
1.58AspMet: 1.58 ± 0.396
3.838AspAsn: 3.838 ± 0.671
1.693AspPro: 1.693 ± 0.45
1.129AspGln: 1.129 ± 0.341
1.467AspArg: 1.467 ± 0.504
3.048AspSer: 3.048 ± 0.649
3.499AspThr: 3.499 ± 0.738
5.08AspVal: 5.08 ± 0.721
1.693AspTrp: 1.693 ± 0.356
2.258AspTyr: 2.258 ± 0.671
0.0AspXaa: 0.0 ± 0.0
Glu
5.192GluAla: 5.192 ± 0.762
0.226GluCys: 0.226 ± 0.186
1.806GluAsp: 1.806 ± 0.524
3.725GluGlu: 3.725 ± 0.838
3.161GluPhe: 3.161 ± 0.582
3.725GluGly: 3.725 ± 0.664
0.79GluHis: 0.79 ± 0.35
3.612GluIle: 3.612 ± 0.669
4.515GluLys: 4.515 ± 1.109
6.999GluLeu: 6.999 ± 1.339
2.032GluMet: 2.032 ± 0.472
3.274GluAsn: 3.274 ± 0.709
1.467GluPro: 1.467 ± 0.304
2.709GluGln: 2.709 ± 0.47
2.596GluArg: 2.596 ± 0.701
1.806GluSer: 1.806 ± 0.409
3.274GluThr: 3.274 ± 0.733
4.628GluVal: 4.628 ± 0.801
0.677GluTrp: 0.677 ± 0.318
2.935GluTyr: 2.935 ± 0.704
0.0GluXaa: 0.0 ± 0.0
Phe
3.161PheAla: 3.161 ± 0.35
0.0PheCys: 0.0 ± 0.0
2.935PheAsp: 2.935 ± 0.811
2.935PheGlu: 2.935 ± 0.577
1.58PhePhe: 1.58 ± 0.514
3.612PheGly: 3.612 ± 0.589
0.452PheHis: 0.452 ± 0.204
2.709PheIle: 2.709 ± 0.472
3.161PheLys: 3.161 ± 0.59
2.032PheLeu: 2.032 ± 0.477
1.355PheMet: 1.355 ± 0.37
3.386PheAsn: 3.386 ± 0.504
0.903PhePro: 0.903 ± 0.281
0.452PheGln: 0.452 ± 0.226
1.129PheArg: 1.129 ± 0.488
3.048PheSer: 3.048 ± 0.588
3.951PheThr: 3.951 ± 0.758
3.386PheVal: 3.386 ± 0.561
0.903PheTrp: 0.903 ± 0.283
2.37PheTyr: 2.37 ± 0.585
0.0PheXaa: 0.0 ± 0.0
Gly
5.08GlyAla: 5.08 ± 0.813
0.113GlyCys: 0.113 ± 0.115
5.644GlyAsp: 5.644 ± 0.797
3.612GlyGlu: 3.612 ± 0.578
3.612GlyPhe: 3.612 ± 0.833
4.741GlyGly: 4.741 ± 0.718
0.452GlyHis: 0.452 ± 0.27
5.644GlyIle: 5.644 ± 1.082
5.757GlyLys: 5.757 ± 0.928
5.983GlyLeu: 5.983 ± 1.143
2.483GlyMet: 2.483 ± 0.503
4.628GlyAsn: 4.628 ± 0.976
0.564GlyPro: 0.564 ± 0.265
2.145GlyGln: 2.145 ± 0.454
3.612GlyArg: 3.612 ± 0.492
4.289GlySer: 4.289 ± 0.733
5.192GlyThr: 5.192 ± 0.912
5.983GlyVal: 5.983 ± 0.887
1.467GlyTrp: 1.467 ± 0.415
2.596GlyTyr: 2.596 ± 0.522
0.0GlyXaa: 0.0 ± 0.0
His
1.242HisAla: 1.242 ± 0.372
0.0HisCys: 0.0 ± 0.0
0.677HisAsp: 0.677 ± 0.325
0.79HisGlu: 0.79 ± 0.253
0.452HisPhe: 0.452 ± 0.253
1.242HisGly: 1.242 ± 0.46
0.113HisHis: 0.113 ± 0.141
0.677HisIle: 0.677 ± 0.335
1.129HisLys: 1.129 ± 0.37
1.016HisLeu: 1.016 ± 0.392
0.113HisMet: 0.113 ± 0.115
0.452HisAsn: 0.452 ± 0.226
1.016HisPro: 1.016 ± 0.313
0.564HisGln: 0.564 ± 0.239
0.339HisArg: 0.339 ± 0.19
0.79HisSer: 0.79 ± 0.295
1.016HisThr: 1.016 ± 0.306
0.903HisVal: 0.903 ± 0.295
0.113HisTrp: 0.113 ± 0.115
0.226HisTyr: 0.226 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
5.644IleAla: 5.644 ± 0.684
0.0IleCys: 0.0 ± 0.0
3.048IleAsp: 3.048 ± 0.546
3.612IleGlu: 3.612 ± 0.549
1.58IlePhe: 1.58 ± 0.424
4.741IleGly: 4.741 ± 0.908
1.129IleHis: 1.129 ± 0.34
3.612IleIle: 3.612 ± 0.705
5.08IleLys: 5.08 ± 0.788
3.048IleLeu: 3.048 ± 0.684
1.919IleMet: 1.919 ± 0.418
3.386IleAsn: 3.386 ± 0.573
1.58IlePro: 1.58 ± 0.36
2.709IleGln: 2.709 ± 0.572
2.258IleArg: 2.258 ± 0.538
4.289IleSer: 4.289 ± 0.622
3.499IleThr: 3.499 ± 0.503
4.402IleVal: 4.402 ± 0.765
2.032IleTrp: 2.032 ± 1.07
2.709IleTyr: 2.709 ± 0.494
0.0IleXaa: 0.0 ± 0.0
Lys
5.531LysAla: 5.531 ± 1.066
0.339LysCys: 0.339 ± 0.222
3.725LysAsp: 3.725 ± 0.638
3.725LysGlu: 3.725 ± 0.938
3.725LysPhe: 3.725 ± 0.675
2.822LysGly: 2.822 ± 0.466
0.79LysHis: 0.79 ± 0.263
4.064LysIle: 4.064 ± 0.712
3.386LysLys: 3.386 ± 0.63
5.192LysLeu: 5.192 ± 0.565
2.258LysMet: 2.258 ± 0.508
4.967LysAsn: 4.967 ± 0.795
2.145LysPro: 2.145 ± 0.52
3.951LysGln: 3.951 ± 0.667
2.822LysArg: 2.822 ± 0.62
3.612LysSer: 3.612 ± 0.703
4.515LysThr: 4.515 ± 0.682
5.305LysVal: 5.305 ± 0.878
0.903LysTrp: 0.903 ± 0.391
2.596LysTyr: 2.596 ± 0.768
0.0LysXaa: 0.0 ± 0.0
Leu
4.628LeuAla: 4.628 ± 0.946
0.226LeuCys: 0.226 ± 0.283
5.305LeuAsp: 5.305 ± 0.875
4.967LeuGlu: 4.967 ± 0.927
2.483LeuPhe: 2.483 ± 0.477
4.402LeuGly: 4.402 ± 0.541
1.467LeuHis: 1.467 ± 0.504
3.838LeuIle: 3.838 ± 0.731
4.177LeuLys: 4.177 ± 0.713
5.08LeuLeu: 5.08 ± 0.814
2.37LeuMet: 2.37 ± 0.52
4.741LeuAsn: 4.741 ± 0.598
3.161LeuPro: 3.161 ± 0.732
3.161LeuGln: 3.161 ± 0.583
3.725LeuArg: 3.725 ± 0.984
4.628LeuSer: 4.628 ± 0.532
6.095LeuThr: 6.095 ± 1.002
3.612LeuVal: 3.612 ± 0.578
1.129LeuTrp: 1.129 ± 0.414
2.258LeuTyr: 2.258 ± 0.666
0.0LeuXaa: 0.0 ± 0.0
Met
2.596MetAla: 2.596 ± 0.473
0.0MetCys: 0.0 ± 0.0
1.693MetAsp: 1.693 ± 0.374
1.58MetGlu: 1.58 ± 0.391
0.452MetPhe: 0.452 ± 0.239
1.355MetGly: 1.355 ± 0.516
0.79MetHis: 0.79 ± 0.287
1.467MetIle: 1.467 ± 0.382
1.693MetLys: 1.693 ± 0.408
2.596MetLeu: 2.596 ± 0.922
0.79MetMet: 0.79 ± 0.297
2.032MetAsn: 2.032 ± 0.497
0.564MetPro: 0.564 ± 0.26
2.032MetGln: 2.032 ± 0.469
0.452MetArg: 0.452 ± 0.208
1.919MetSer: 1.919 ± 0.425
2.822MetThr: 2.822 ± 0.45
1.919MetVal: 1.919 ± 0.56
0.113MetTrp: 0.113 ± 0.111
0.677MetTyr: 0.677 ± 0.263
0.0MetXaa: 0.0 ± 0.0
Asn
6.095AsnAla: 6.095 ± 0.857
0.339AsnCys: 0.339 ± 0.315
4.967AsnAsp: 4.967 ± 0.724
3.951AsnGlu: 3.951 ± 0.977
3.161AsnPhe: 3.161 ± 0.775
7.676AsnGly: 7.676 ± 1.057
0.79AsnHis: 0.79 ± 0.27
2.822AsnIle: 2.822 ± 0.51
3.274AsnLys: 3.274 ± 0.58
3.725AsnLeu: 3.725 ± 0.555
2.37AsnMet: 2.37 ± 0.454
5.192AsnAsn: 5.192 ± 0.935
2.37AsnPro: 2.37 ± 0.629
1.919AsnGln: 1.919 ± 0.485
2.032AsnArg: 2.032 ± 0.541
4.064AsnSer: 4.064 ± 0.669
4.402AsnThr: 4.402 ± 1.052
4.741AsnVal: 4.741 ± 0.712
1.467AsnTrp: 1.467 ± 0.436
2.258AsnTyr: 2.258 ± 0.521
0.0AsnXaa: 0.0 ± 0.0
Pro
2.483ProAla: 2.483 ± 0.498
0.0ProCys: 0.0 ± 0.0
2.032ProAsp: 2.032 ± 0.662
1.919ProGlu: 1.919 ± 0.431
1.467ProPhe: 1.467 ± 0.417
0.79ProGly: 0.79 ± 0.279
0.226ProHis: 0.226 ± 0.175
1.58ProIle: 1.58 ± 0.493
1.467ProLys: 1.467 ± 0.369
3.048ProLeu: 3.048 ± 0.698
0.339ProMet: 0.339 ± 0.185
1.467ProAsn: 1.467 ± 0.36
0.339ProPro: 0.339 ± 0.18
1.355ProGln: 1.355 ± 0.282
0.79ProArg: 0.79 ± 0.385
2.145ProSer: 2.145 ± 0.519
2.145ProThr: 2.145 ± 0.51
2.145ProVal: 2.145 ± 0.465
0.79ProTrp: 0.79 ± 0.263
1.016ProTyr: 1.016 ± 0.359
0.0ProXaa: 0.0 ± 0.0
Gln
4.515GlnAla: 4.515 ± 0.946
0.0GlnCys: 0.0 ± 0.0
1.242GlnAsp: 1.242 ± 0.479
2.145GlnGlu: 2.145 ± 0.694
1.467GlnPhe: 1.467 ± 0.434
3.386GlnGly: 3.386 ± 0.59
0.903GlnHis: 0.903 ± 0.35
2.145GlnIle: 2.145 ± 0.457
2.822GlnLys: 2.822 ± 0.522
2.935GlnLeu: 2.935 ± 0.689
1.016GlnMet: 1.016 ± 0.343
2.709GlnAsn: 2.709 ± 0.504
0.903GlnPro: 0.903 ± 0.271
2.145GlnGln: 2.145 ± 0.576
1.355GlnArg: 1.355 ± 0.364
2.145GlnSer: 2.145 ± 0.459
2.483GlnThr: 2.483 ± 0.626
3.161GlnVal: 3.161 ± 0.726
0.452GlnTrp: 0.452 ± 0.231
1.58GlnTyr: 1.58 ± 0.455
0.0GlnXaa: 0.0 ± 0.0
Arg
1.919ArgAla: 1.919 ± 0.567
0.113ArgCys: 0.113 ± 0.125
2.483ArgAsp: 2.483 ± 0.662
2.258ArgGlu: 2.258 ± 0.582
2.145ArgPhe: 2.145 ± 0.68
2.709ArgGly: 2.709 ± 0.513
0.564ArgHis: 0.564 ± 0.229
2.032ArgIle: 2.032 ± 0.653
1.919ArgLys: 1.919 ± 0.461
3.838ArgLeu: 3.838 ± 0.922
1.129ArgMet: 1.129 ± 0.378
2.37ArgAsn: 2.37 ± 0.543
1.016ArgPro: 1.016 ± 0.344
1.129ArgGln: 1.129 ± 0.381
1.919ArgArg: 1.919 ± 0.63
2.37ArgSer: 2.37 ± 0.575
2.37ArgThr: 2.37 ± 0.527
2.483ArgVal: 2.483 ± 0.56
0.677ArgTrp: 0.677 ± 0.317
2.032ArgTyr: 2.032 ± 0.527
0.0ArgXaa: 0.0 ± 0.0
Ser
4.402SerAla: 4.402 ± 0.702
0.564SerCys: 0.564 ± 0.348
3.274SerAsp: 3.274 ± 0.629
4.177SerGlu: 4.177 ± 0.791
2.709SerPhe: 2.709 ± 0.434
6.208SerGly: 6.208 ± 1.227
0.452SerHis: 0.452 ± 0.2
4.628SerIle: 4.628 ± 1.198
4.515SerLys: 4.515 ± 0.731
4.402SerLeu: 4.402 ± 0.774
1.355SerMet: 1.355 ± 0.356
4.854SerAsn: 4.854 ± 0.703
2.032SerPro: 2.032 ± 0.633
2.596SerGln: 2.596 ± 0.588
2.709SerArg: 2.709 ± 0.591
5.192SerSer: 5.192 ± 1.135
4.515SerThr: 4.515 ± 0.821
4.177SerVal: 4.177 ± 0.53
0.79SerTrp: 0.79 ± 0.318
2.483SerTyr: 2.483 ± 0.541
0.0SerXaa: 0.0 ± 0.0
Thr
8.127ThrAla: 8.127 ± 1.226
0.226ThrCys: 0.226 ± 0.23
3.386ThrAsp: 3.386 ± 0.768
2.822ThrGlu: 2.822 ± 0.595
3.274ThrPhe: 3.274 ± 0.463
4.177ThrGly: 4.177 ± 0.924
0.79ThrHis: 0.79 ± 0.362
4.741ThrIle: 4.741 ± 0.788
3.725ThrLys: 3.725 ± 0.494
3.274ThrLeu: 3.274 ± 0.51
1.355ThrMet: 1.355 ± 0.329
3.725ThrAsn: 3.725 ± 0.637
2.709ThrPro: 2.709 ± 0.483
2.37ThrGln: 2.37 ± 0.55
2.822ThrArg: 2.822 ± 0.486
5.192ThrSer: 5.192 ± 1.05
5.418ThrThr: 5.418 ± 0.766
5.192ThrVal: 5.192 ± 0.932
0.564ThrTrp: 0.564 ± 0.207
4.177ThrTyr: 4.177 ± 0.844
0.0ThrXaa: 0.0 ± 0.0
Val
6.095ValAla: 6.095 ± 1.022
0.0ValCys: 0.0 ± 0.0
5.757ValAsp: 5.757 ± 1.039
3.951ValGlu: 3.951 ± 0.695
2.709ValPhe: 2.709 ± 0.522
4.741ValGly: 4.741 ± 0.664
0.79ValHis: 0.79 ± 0.257
3.499ValIle: 3.499 ± 0.747
6.208ValLys: 6.208 ± 1.028
3.386ValLeu: 3.386 ± 0.79
1.129ValMet: 1.129 ± 0.277
5.531ValAsn: 5.531 ± 0.89
2.032ValPro: 2.032 ± 0.572
3.048ValGln: 3.048 ± 0.45
1.58ValArg: 1.58 ± 0.477
7.224ValSer: 7.224 ± 1.067
4.402ValThr: 4.402 ± 0.765
3.725ValVal: 3.725 ± 0.706
1.129ValTrp: 1.129 ± 0.522
3.161ValTyr: 3.161 ± 0.624
0.0ValXaa: 0.0 ± 0.0
Trp
1.016TrpAla: 1.016 ± 0.409
0.113TrpCys: 0.113 ± 0.141
0.79TrpAsp: 0.79 ± 0.285
1.129TrpGlu: 1.129 ± 0.346
0.79TrpPhe: 0.79 ± 0.434
1.693TrpGly: 1.693 ± 0.327
0.226TrpHis: 0.226 ± 0.137
1.129TrpIle: 1.129 ± 0.571
1.016TrpLys: 1.016 ± 0.345
1.806TrpLeu: 1.806 ± 0.461
0.0TrpMet: 0.0 ± 0.0
2.032TrpAsn: 2.032 ± 1.065
0.0TrpPro: 0.0 ± 0.0
0.903TrpGln: 0.903 ± 0.317
0.903TrpArg: 0.903 ± 0.349
1.58TrpSer: 1.58 ± 0.719
1.58TrpThr: 1.58 ± 0.417
0.79TrpVal: 0.79 ± 0.408
0.452TrpTrp: 0.452 ± 0.197
0.452TrpTyr: 0.452 ± 0.242
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.274TyrAla: 3.274 ± 0.656
0.113TyrCys: 0.113 ± 0.125
2.032TyrAsp: 2.032 ± 0.509
2.483TyrGlu: 2.483 ± 0.521
2.37TyrPhe: 2.37 ± 0.48
2.596TyrGly: 2.596 ± 0.702
0.339TyrHis: 0.339 ± 0.155
2.258TyrIle: 2.258 ± 0.618
3.951TyrLys: 3.951 ± 0.843
3.161TyrLeu: 3.161 ± 0.627
1.242TyrMet: 1.242 ± 0.293
2.483TyrAsn: 2.483 ± 0.6
1.355TyrPro: 1.355 ± 0.358
1.467TyrGln: 1.467 ± 0.47
1.806TyrArg: 1.806 ± 0.651
2.935TyrSer: 2.935 ± 0.587
2.596TyrThr: 2.596 ± 0.498
2.822TyrVal: 2.822 ± 0.488
0.564TyrTrp: 0.564 ± 0.257
2.258TyrTyr: 2.258 ± 0.601
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 35 proteins (8860 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski