Wuhan Fly Virus 1
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5KKS7|A0A0B5KKS7_9VIRU Nucleocapsid protein OS=Wuhan Fly Virus 1 OX=1608101 GN=N PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 4.56 EE3 pKa = 4.15 VEE5 pKa = 4.97 SEE7 pKa = 4.48 LSQEE11 pKa = 4.26 TPNEE15 pKa = 3.77 YY16 pKa = 10.09 RR17 pKa = 11.84 FKK19 pKa = 11.29 EE20 pKa = 4.22 EE21 pKa = 3.94 VLHH24 pKa = 7.53 SILDD28 pKa = 3.74 EE29 pKa = 4.08 QANRR33 pKa = 11.84 VPPGEE38 pKa = 4.07 HH39 pKa = 6.17 VNLGRR44 pKa = 11.84 QTGVYY49 pKa = 10.28 DD50 pKa = 3.95 STSVTYY56 pKa = 10.94 NLIPYY61 pKa = 7.08 EE62 pKa = 4.21 VKK64 pKa = 10.43 VEE66 pKa = 3.93 EE67 pKa = 4.66 RR68 pKa = 11.84 RR69 pKa = 11.84 ITTRR73 pKa = 11.84 NDD75 pKa = 2.59 FTLIIQRR82 pKa = 11.84 EE83 pKa = 4.1 NLEE86 pKa = 4.35 GVGSIGSSILNGVIRR101 pKa = 11.84 AKK103 pKa = 10.32 DD104 pKa = 3.62 VRR106 pKa = 11.84 TFPHH110 pKa = 7.56 DD111 pKa = 3.37 FTFNPITSSTDD122 pKa = 3.0 VRR124 pKa = 11.84 ISDD127 pKa = 3.62 ILSGVSTPLSNLTPDD142 pKa = 3.06 FMEE145 pKa = 4.29 YY146 pKa = 10.77 FNGNLVVMEE155 pKa = 4.77 FATTQQDD162 pKa = 3.63 SAIRR166 pKa = 11.84 TVLNQKK172 pKa = 8.43 VDD174 pKa = 2.99 KK175 pKa = 10.94 YY176 pKa = 10.7 RR177 pKa = 11.84 IPLRR181 pKa = 11.84 EE182 pKa = 3.82 AARR185 pKa = 11.84 QYY187 pKa = 11.03 SIQNNMYY194 pKa = 10.25 LVVHH198 pKa = 6.43 LCVIVVSEE206 pKa = 4.15 TRR208 pKa = 11.84 VASTHH213 pKa = 6.71 RR214 pKa = 11.84 LPQSIIDD221 pKa = 3.77 EE222 pKa = 4.03 LTYY225 pKa = 10.26 RR226 pKa = 11.84 YY227 pKa = 9.5 RR228 pKa = 11.84 LSYY231 pKa = 10.34 SIQSKK236 pKa = 10.3 ALSLMGRR243 pKa = 11.84 DD244 pKa = 3.9 RR245 pKa = 11.84 DD246 pKa = 4.53 LIDD249 pKa = 4.53 DD250 pKa = 4.05 NKK252 pKa = 10.33 EE253 pKa = 3.11 VDD255 pKa = 3.28 MFKK258 pKa = 10.25 TFFKK262 pKa = 10.55 SIPVEE267 pKa = 3.91 DD268 pKa = 4.0 LGGEE272 pKa = 4.35 FSLEE276 pKa = 4.03 NYY278 pKa = 9.79 NEE280 pKa = 3.95 LMQDD284 pKa = 4.03 LEE286 pKa = 4.31 PQAEE290 pKa = 4.15 EE291 pKa = 4.08 EE292 pKa = 4.2 LVKK295 pKa = 10.81 KK296 pKa = 10.56 IIKK299 pKa = 7.06 TAKK302 pKa = 9.36 SQIHH306 pKa = 6.2 EE307 pKa = 4.61 KK308 pKa = 9.76 IQDD311 pKa = 3.49 YY312 pKa = 9.12 EE313 pKa = 4.63 ANYY316 pKa = 9.87 QPNKK320 pKa = 10.64 GSDD323 pKa = 3.3 NPNFLDD329 pKa = 4.22 CRR331 pKa = 11.84 MAFMKK336 pKa = 10.97 YY337 pKa = 10.05 LDD339 pKa = 5.32 DD340 pKa = 3.87 YY341 pKa = 11.92 VEE343 pKa = 4.11 QNKK346 pKa = 9.66 QDD348 pKa = 3.73 GLRR351 pKa = 11.84 SDD353 pKa = 4.17 MKK355 pKa = 11.49 SMVQFPFICLRR366 pKa = 11.84 RR367 pKa = 11.84 VSRR370 pKa = 11.84 YY371 pKa = 9.45 DD372 pKa = 3.48 FKK374 pKa = 12.1 SMIDD378 pKa = 3.29 NNAFPRR384 pKa = 11.84 YY385 pKa = 9.87 DD386 pKa = 3.8 EE387 pKa = 4.68 EE388 pKa = 4.15 NHH390 pKa = 6.0 LLRR393 pKa = 11.84 FVRR396 pKa = 11.84 TVVMKK401 pKa = 10.44 IASGDD406 pKa = 3.33 IYY408 pKa = 11.4 VPEE411 pKa = 4.25 TNEE414 pKa = 5.35 DD415 pKa = 3.52 DD416 pKa = 4.05 TDD418 pKa = 3.78 SLKK421 pKa = 11.02 YY422 pKa = 10.29 LFSEE426 pKa = 4.79 AGVEE430 pKa = 3.88 NHH432 pKa = 6.24 NLFEE436 pKa = 4.13 THH438 pKa = 6.42 IMSNPGKK445 pKa = 9.8 YY446 pKa = 9.65 SKK448 pKa = 10.6 KK449 pKa = 9.39 SQHH452 pKa = 5.7 QIEE455 pKa = 4.58 NRR457 pKa = 11.84 SKK459 pKa = 7.47 YY460 pKa = 9.86 HH461 pKa = 6.18 RR462 pKa = 11.84 MIVEE466 pKa = 3.84 IEE468 pKa = 4.13 KK469 pKa = 10.56 EE470 pKa = 4.06 DD471 pKa = 4.16 EE472 pKa = 3.91 IEE474 pKa = 3.89 MAKK477 pKa = 10.61 YY478 pKa = 10.12 GIYY481 pKa = 9.91 GKK483 pKa = 10.35 KK484 pKa = 10.25 YY485 pKa = 9.87 SDD487 pKa = 3.03 NHH489 pKa = 4.14 EE490 pKa = 4.15 VKK492 pKa = 10.45 RR493 pKa = 11.84 YY494 pKa = 9.99 RR495 pKa = 11.84 KK496 pKa = 8.87 DD497 pKa = 2.91 KK498 pKa = 11.07 KK499 pKa = 10.73 RR500 pKa = 11.84 FFCPKK505 pKa = 9.25 EE506 pKa = 4.07 TDD508 pKa = 3.1 TSDD511 pKa = 4.01 LDD513 pKa = 4.74 KK514 pKa = 11.28 ILDD517 pKa = 3.86 NDD519 pKa = 4.27 DD520 pKa = 4.33 LVTEE524 pKa = 5.0 LFDD527 pKa = 4.69 KK528 pKa = 11.0 SSSLNEE534 pKa = 3.4 TDD536 pKa = 3.32 LDD538 pKa = 3.84 VRR540 pKa = 11.84 GLIFEE545 pKa = 4.35 SLSLHH550 pKa = 5.07 TTPEE554 pKa = 3.91 EE555 pKa = 3.85 LKK557 pKa = 10.45 NASSEE562 pKa = 4.46 YY563 pKa = 9.88 YY564 pKa = 10.21 LDD566 pKa = 3.71 VMEE569 pKa = 4.91 STLGTYY575 pKa = 11.02 ASMISDD581 pKa = 3.62 IGVEE585 pKa = 3.76 LAISIKK591 pKa = 9.25 QNCKK595 pKa = 7.43 SHH597 pKa = 5.84 EE598 pKa = 4.48 FIVKK602 pKa = 9.79 KK603 pKa = 10.64 LKK605 pKa = 10.68 NFNLWLIIKK614 pKa = 7.03 PTKK617 pKa = 8.94 STSHH621 pKa = 6.77 IFFCAFTYY629 pKa = 10.54 DD630 pKa = 4.2 SEE632 pKa = 4.67 LCNGLSSSCKK642 pKa = 8.19 PEE644 pKa = 3.6 AMRR647 pKa = 11.84 SVFKK651 pKa = 10.13 TLHH654 pKa = 6.39 RR655 pKa = 11.84 DD656 pKa = 3.01 GSFLYY661 pKa = 10.57 TEE663 pKa = 5.6 FISLNMSKK671 pKa = 10.48 LVNWVLCEE679 pKa = 3.8 SRR681 pKa = 11.84 FYY683 pKa = 10.76 SACAFWQNSYY693 pKa = 10.8 GIPVSMGQEE702 pKa = 3.22 ISEE705 pKa = 4.25 THH707 pKa = 5.09 KK708 pKa = 10.85 QGLRR712 pKa = 11.84 EE713 pKa = 4.08 AQKK716 pKa = 8.26 MTLITLMVNLIDD728 pKa = 3.66 KK729 pKa = 11.23 AEE731 pKa = 4.18 VEE733 pKa = 4.44 EE734 pKa = 4.2 HH735 pKa = 5.89 MSMLRR740 pKa = 11.84 YY741 pKa = 8.29 ITLEE745 pKa = 3.95 GLVAAPNKK753 pKa = 9.41 PNPHH757 pKa = 6.01 KK758 pKa = 10.27 MFEE761 pKa = 4.23 KK762 pKa = 10.22 FSMSIKK768 pKa = 10.52 SRR770 pKa = 11.84 FTLWVIRR777 pKa = 11.84 KK778 pKa = 8.14 LATFASHH785 pKa = 6.66 VADD788 pKa = 4.67 GNIKK792 pKa = 9.78 MIIEE796 pKa = 4.78 RR797 pKa = 11.84 IEE799 pKa = 4.36 DD800 pKa = 3.57 VATNTLNRR808 pKa = 11.84 KK809 pKa = 8.68 LWTGFMNPYY818 pKa = 10.0 LNQPVSSPQLMINLFYY834 pKa = 10.97 LGYY837 pKa = 10.52 LKK839 pKa = 11.01 NKK841 pKa = 10.36 DD842 pKa = 3.88 EE843 pKa = 4.22 YY844 pKa = 10.72 PEE846 pKa = 4.42 NNTLSKK852 pKa = 10.51 LYY854 pKa = 10.54 EE855 pKa = 4.87 KK856 pKa = 10.54 ILVIEE861 pKa = 4.78 DD862 pKa = 3.62 EE863 pKa = 4.2 LTEE866 pKa = 4.12 EE867 pKa = 4.23 VEE869 pKa = 4.21 SRR871 pKa = 11.84 IGKK874 pKa = 7.23 TDD876 pKa = 3.6 KK877 pKa = 10.71 PWGSNTKK884 pKa = 9.99 HH885 pKa = 6.31 EE886 pKa = 4.49 YY887 pKa = 10.27 NISLIKK893 pKa = 10.5 LATKK897 pKa = 10.15 KK898 pKa = 10.41 CVEE901 pKa = 4.63 KK902 pKa = 10.58 FQSSNPGFLLDD913 pKa = 5.21 LEE915 pKa = 4.45 NLVLSNLARR924 pKa = 11.84 TGLDD928 pKa = 3.16 PTFTTLKK935 pKa = 10.24 ASSSFNSDD943 pKa = 2.66 LFALRR948 pKa = 11.84 SVTTGYY954 pKa = 10.9 HH955 pKa = 5.28 RR956 pKa = 11.84 MKK958 pKa = 10.6 VVEE961 pKa = 4.64 ALTKK965 pKa = 10.52 KK966 pKa = 10.48 LNQEE970 pKa = 4.1 DD971 pKa = 4.19 YY972 pKa = 11.06 RR973 pKa = 11.84 LVDD976 pKa = 3.35 VLPEE980 pKa = 3.7 VAEE983 pKa = 4.17 EE984 pKa = 4.17 VFEE987 pKa = 4.57 RR988 pKa = 11.84 KK989 pKa = 9.63 YY990 pKa = 11.42 LLIDD994 pKa = 3.64 IFQKK998 pKa = 10.19 NQHH1001 pKa = 5.74 GGLRR1005 pKa = 11.84 EE1006 pKa = 3.95 IYY1008 pKa = 10.64 VLGLFDD1014 pKa = 6.55 RR1015 pKa = 11.84 IIQYY1019 pKa = 10.37 VIEE1022 pKa = 4.8 SISRR1026 pKa = 11.84 TICTSFPGEE1035 pKa = 4.43 TMTHH1039 pKa = 6.67 PNNKK1043 pKa = 9.66 RR1044 pKa = 11.84 IIPEE1048 pKa = 3.87 SHH1050 pKa = 6.49 HH1051 pKa = 6.74 AKK1053 pKa = 10.28 CKK1055 pKa = 9.16 QRR1057 pKa = 11.84 YY1058 pKa = 7.31 SNSYY1062 pKa = 7.92 ITFGLSADD1070 pKa = 3.48 ASKK1073 pKa = 10.47 WSQNHH1078 pKa = 5.07 YY1079 pKa = 9.32 AHH1081 pKa = 7.37 KK1082 pKa = 10.04 FSIMLFRR1089 pKa = 11.84 FLPKK1093 pKa = 10.29 YY1094 pKa = 7.85 MHH1096 pKa = 6.41 GFIWQALHH1104 pKa = 5.84 LWKK1107 pKa = 10.53 DD1108 pKa = 3.27 KK1109 pKa = 11.13 RR1110 pKa = 11.84 IMLNPKK1116 pKa = 9.5 LIHH1119 pKa = 6.66 ILDD1122 pKa = 4.39 DD1123 pKa = 3.58 STIEE1127 pKa = 4.09 GFYY1130 pKa = 10.72 SEE1132 pKa = 5.94 PIQKK1136 pKa = 10.57 LYY1138 pKa = 11.03 DD1139 pKa = 4.04 CYY1141 pKa = 10.69 HH1142 pKa = 5.98 GRR1144 pKa = 11.84 INLKK1148 pKa = 9.44 WYY1150 pKa = 7.62 THH1152 pKa = 6.29 GEE1154 pKa = 4.2 SFIKK1158 pKa = 9.89 TRR1160 pKa = 11.84 TGMMQGILHH1169 pKa = 5.41 YY1170 pKa = 9.8 TSSLLHH1176 pKa = 5.87 AVVNEE1181 pKa = 3.83 FSKK1184 pKa = 10.64 MVMLNWCSVLQDD1196 pKa = 3.31 SRR1198 pKa = 11.84 GPPEE1202 pKa = 3.99 IDD1204 pKa = 2.99 MLEE1207 pKa = 4.31 SSDD1210 pKa = 3.87 DD1211 pKa = 3.67 SAMLFSLPIDD1221 pKa = 4.64 DD1222 pKa = 4.48 NSKK1225 pKa = 10.54 SLRR1228 pKa = 11.84 KK1229 pKa = 9.71 AHH1231 pKa = 6.78 ILAQFAQEE1239 pKa = 3.55 IKK1241 pKa = 10.31 EE1242 pKa = 4.04 IIGRR1246 pKa = 11.84 EE1247 pKa = 3.69 VSIINSIKK1255 pKa = 7.49 TTSISLFFYY1264 pKa = 10.21 EE1265 pKa = 5.19 FNSEE1269 pKa = 4.44 FKK1271 pKa = 10.73 FGSNHH1276 pKa = 4.96 YY1277 pKa = 10.36 RR1278 pKa = 11.84 PDD1280 pKa = 3.17 IKK1282 pKa = 11.03 FMISLPLISEE1292 pKa = 4.05 QEE1294 pKa = 3.83 ALIARR1299 pKa = 11.84 QEE1301 pKa = 4.03 EE1302 pKa = 4.93 WSTLLTQYY1310 pKa = 10.46 IEE1312 pKa = 4.53 NGGSTWPVHH1321 pKa = 6.24 CGQVSQALIHH1331 pKa = 5.6 YY1332 pKa = 8.93 RR1333 pKa = 11.84 LMGSSVCKK1341 pKa = 10.11 EE1342 pKa = 3.99 FEE1344 pKa = 4.37 DD1345 pKa = 4.43 FTFLALHH1352 pKa = 6.66 LKK1354 pKa = 10.53 DD1355 pKa = 3.77 PSQGFFLMDD1364 pKa = 4.87 PIISSGLLGFKK1375 pKa = 10.58 YY1376 pKa = 10.47 NLWNACQKK1384 pKa = 9.85 TNLGLLYY1391 pKa = 10.37 KK1392 pKa = 10.07 EE1393 pKa = 4.07 YY1394 pKa = 10.89 LFRR1397 pKa = 11.84 STDD1400 pKa = 3.16 GSEE1403 pKa = 4.15 EE1404 pKa = 4.03 KK1405 pKa = 11.24 LEE1407 pKa = 4.16 VTQSGSLSRR1416 pKa = 11.84 TVKK1419 pKa = 10.66 LIYY1422 pKa = 10.17 GDD1424 pKa = 3.29 RR1425 pKa = 11.84 RR1426 pKa = 11.84 KK1427 pKa = 9.31 WLRR1430 pKa = 11.84 MLEE1433 pKa = 4.18 RR1434 pKa = 11.84 IGASEE1439 pKa = 3.86 TWKK1442 pKa = 11.06 EE1443 pKa = 4.1 EE1444 pKa = 3.62 INEE1447 pKa = 4.18 DD1448 pKa = 4.33 FKK1450 pKa = 11.24 MLYY1453 pKa = 9.99 IGVKK1457 pKa = 10.26 SNADD1461 pKa = 3.66 LRR1463 pKa = 11.84 RR1464 pKa = 11.84 MISLKK1469 pKa = 9.26 MHH1471 pKa = 6.35 TSGVAEE1477 pKa = 4.72 SISKK1481 pKa = 10.9 GNVISRR1487 pKa = 11.84 TIASSVFILTFQCLTRR1503 pKa = 11.84 MRR1505 pKa = 11.84 EE1506 pKa = 4.05 WQSEE1510 pKa = 4.3 DD1511 pKa = 2.99 TGGKK1515 pKa = 9.0 PKK1517 pKa = 10.5 QSLFDD1522 pKa = 4.69 LLTDD1526 pKa = 4.3 SIKK1529 pKa = 10.19 TISKK1533 pKa = 9.0 ADD1535 pKa = 3.73 RR1536 pKa = 11.84 RR1537 pKa = 11.84 IDD1539 pKa = 3.63 EE1540 pKa = 4.28 SEE1542 pKa = 4.05 LRR1544 pKa = 11.84 VLFPFHH1550 pKa = 7.98 DD1551 pKa = 4.33 DD1552 pKa = 3.28 FTRR1555 pKa = 11.84 IRR1557 pKa = 11.84 EE1558 pKa = 4.23 MIDD1561 pKa = 2.96 GLGNISLKK1569 pKa = 10.65 PVFKK1573 pKa = 10.4 DD1574 pKa = 3.1 YY1575 pKa = 10.88 RR1576 pKa = 11.84 RR1577 pKa = 11.84 RR1578 pKa = 11.84 ITNIEE1583 pKa = 3.68 VSTSVKK1589 pKa = 10.11 ISNLPLKK1596 pKa = 10.48 EE1597 pKa = 3.85 IVTQVWFPGEE1607 pKa = 3.77 KK1608 pKa = 8.66 HH1609 pKa = 6.16 TIISPRR1615 pKa = 11.84 TAMVLFYY1622 pKa = 10.01 EE1623 pKa = 4.53 HH1624 pKa = 6.83 QNTIHH1629 pKa = 7.17 WLNKK1633 pKa = 9.79 DD1634 pKa = 3.56 PKK1636 pKa = 9.93 KK1637 pKa = 9.35 TIQDD1641 pKa = 3.72 SPFDD1645 pKa = 4.3 DD1646 pKa = 4.96 AVSLYY1651 pKa = 10.56 NWISSVSNKK1660 pKa = 9.98 DD1661 pKa = 2.96 RR1662 pKa = 11.84 VVRR1665 pKa = 11.84 LLGTPIISHH1674 pKa = 6.56 HH1675 pKa = 6.35 GKK1677 pKa = 10.01 SSLMSAMMLNFQTNFKK1693 pKa = 10.63 LEE1695 pKa = 4.15 RR1696 pKa = 11.84 SDD1698 pKa = 3.3 DD1699 pKa = 3.4 TSRR1702 pKa = 11.84 YY1703 pKa = 9.65 IEE1705 pKa = 3.94 THH1707 pKa = 5.45 SIKK1710 pKa = 9.9 TVLYY1714 pKa = 8.29 MLSRR1718 pKa = 11.84 SPFSEE1723 pKa = 3.68 QRR1725 pKa = 11.84 AVSLMEE1731 pKa = 3.95 LALEE1735 pKa = 4.05 HH1736 pKa = 6.75 SEE1738 pKa = 4.05 YY1739 pKa = 10.79 QIEE1742 pKa = 4.35 FQSKK1746 pKa = 7.73 NRR1748 pKa = 11.84 KK1749 pKa = 8.55 SRR1751 pKa = 11.84 EE1752 pKa = 3.61 NTLAIIQKK1760 pKa = 7.98 TLAGGTSQEE1769 pKa = 3.81 ISTMIEE1775 pKa = 4.03 DD1776 pKa = 3.49 NRR1778 pKa = 11.84 MGIIGGFTTRR1788 pKa = 11.84 GSPSKK1793 pKa = 9.66 RR1794 pKa = 11.84 TSLSGPRR1801 pKa = 11.84 ARR1803 pKa = 11.84 YY1804 pKa = 8.75 EE1805 pKa = 3.9 GTAIWEE1811 pKa = 4.23 GLIHH1815 pKa = 5.91 GVKK1818 pKa = 10.32 VKK1820 pKa = 10.74 LVTNSVGLSKK1830 pKa = 11.29 DD1831 pKa = 3.04 EE1832 pKa = 5.35 DD1833 pKa = 3.86 YY1834 pKa = 11.42 TGVLRR1839 pKa = 11.84 RR1840 pKa = 11.84 IEE1842 pKa = 3.91 ISEE1845 pKa = 4.06 ITHH1848 pKa = 6.53 FMYY1851 pKa = 10.44 ISNDD1855 pKa = 2.29 IRR1857 pKa = 11.84 TLCKK1861 pKa = 10.16 EE1862 pKa = 4.11 MKK1864 pKa = 10.35 LKK1866 pKa = 10.24 IDD1868 pKa = 3.42 TYY1870 pKa = 11.89 DD1871 pKa = 3.26 NVKK1874 pKa = 10.33 LGRR1877 pKa = 11.84 STTFGLISSGLSQRR1891 pKa = 11.84 FGVPVSKK1898 pKa = 10.58 LSKK1901 pKa = 9.46 EE1902 pKa = 3.9 LQFNIEE1908 pKa = 4.08 GVSNLRR1914 pKa = 11.84 VDD1916 pKa = 3.33 IRR1918 pKa = 11.84 RR1919 pKa = 11.84 NAIRR1923 pKa = 11.84 LIADD1927 pKa = 3.26 MRR1929 pKa = 11.84 DD1930 pKa = 3.01 WSQSSVTLLSYY1941 pKa = 9.98 VIQEE1945 pKa = 3.64 SDD1947 pKa = 3.88 YY1948 pKa = 10.5 YY1949 pKa = 11.27 DD1950 pKa = 3.44 SDD1952 pKa = 3.7 RR1953 pKa = 11.84 AIPEE1957 pKa = 4.08 SFIEE1961 pKa = 4.92 RR1962 pKa = 11.84 IPEE1965 pKa = 4.21 SIRR1968 pKa = 11.84 KK1969 pKa = 8.27 QVTLWIRR1976 pKa = 11.84 DD1977 pKa = 3.35 KK1978 pKa = 11.11 PADD1981 pKa = 3.72 VRR1983 pKa = 11.84 DD1984 pKa = 3.85 ASCLIEE1990 pKa = 4.22 AVSGIFGRR1998 pKa = 11.84 PKK2000 pKa = 10.84 EE2001 pKa = 4.12 EE2002 pKa = 3.85 LTDD2005 pKa = 3.78 EE2006 pKa = 4.79 DD2007 pKa = 3.67 RR2008 pKa = 11.84 HH2009 pKa = 5.53 KK2010 pKa = 11.32 SKK2012 pKa = 10.77 VLRR2015 pKa = 11.84 EE2016 pKa = 4.07 VFVAAMIRR2024 pKa = 11.84 LGINQSKK2031 pKa = 10.83 DD2032 pKa = 3.24 NNQFAIAATRR2042 pKa = 11.84 FKK2044 pKa = 10.75 PEE2046 pKa = 4.02 PEE2048 pKa = 4.04 EE2049 pKa = 4.15 FDD2051 pKa = 4.18 LDD2053 pKa = 4.07 EE2054 pKa = 5.43 LRR2056 pKa = 11.84 VEE2058 pKa = 4.88 YY2059 pKa = 10.81 DD2060 pKa = 3.62 PAWEE2064 pKa = 5.36 DD2065 pKa = 3.65 EE2066 pKa = 4.0 FDD2068 pKa = 4.53 LNISEE2073 pKa = 4.65 EE2074 pKa = 4.23 GSMDD2078 pKa = 3.94 QEE2080 pKa = 4.29 DD2081 pKa = 4.24 QEE2083 pKa = 5.63 VSTNWDD2089 pKa = 2.95 IDD2091 pKa = 3.58 MMLGDD2096 pKa = 5.47 DD2097 pKa = 5.32 APDD2100 pKa = 3.46 LLGLVEE2106 pKa = 4.5 FLEE2109 pKa = 4.85 DD2110 pKa = 3.25 NTRR2113 pKa = 11.84 IQLSDD2118 pKa = 2.9 ASIFRR2123 pKa = 11.84 RR2124 pKa = 11.84 HH2125 pKa = 6.08 KK2126 pKa = 11.1 LFDD2129 pKa = 3.62 NYY2131 pKa = 11.12 AKK2133 pKa = 10.89 NILTQFAIPNVSSCFNLARR2152 pKa = 11.84 VRR2154 pKa = 11.84 EE2155 pKa = 4.39 STKK2158 pKa = 10.13 SLRR2161 pKa = 11.84 SAVAVYY2167 pKa = 10.51 VGVSPDD2173 pKa = 3.8 SIHH2176 pKa = 8.13 VEE2178 pKa = 4.1 DD2179 pKa = 5.43 SRR2181 pKa = 11.84 DD2182 pKa = 3.62 EE2183 pKa = 4.14 PSSINFNFDD2192 pKa = 2.58
Molecular weight: 252.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.727
IPC2_protein 5.664
IPC_protein 5.715
Toseland 6.097
ProMoST 6.008
Dawson 5.931
Bjellqvist 5.97
Wikipedia 5.931
Rodwell 5.919
Grimsley 6.249
Solomon 5.931
Lehninger 5.919
Nozaki 6.186
DTASelect 6.364
Thurlkill 6.351
EMBOSS 6.313
Sillero 6.287
Patrickios 4.304
IPC_peptide 5.957
IPC2_peptide 6.262
IPC2.peptide.svr19 6.275
Protein with the highest isoelectric point:
>tr|A0A0B5KU03|A0A0B5KU03_9VIRU Glycoprotein OS=Wuhan Fly Virus 1 OX=1608101 GN=G PE=4 SV=1
MM1 pKa = 6.77 STDD4 pKa = 2.87 WEE6 pKa = 4.27 QIYY9 pKa = 10.1 QEE11 pKa = 4.38 VLSQLEE17 pKa = 4.1 SDD19 pKa = 4.22 GYY21 pKa = 10.1 EE22 pKa = 3.93 ALIALQRR29 pKa = 11.84 TFAYY33 pKa = 10.16 QGFDD37 pKa = 3.42 PVVTASFIKK46 pKa = 10.42 KK47 pKa = 9.88 KK48 pKa = 10.61 EE49 pKa = 3.67 PTAQIRR55 pKa = 11.84 AKK57 pKa = 10.41 DD58 pKa = 3.75 IYY60 pKa = 11.07 TMIILFITRR69 pKa = 11.84 GNKK72 pKa = 9.04 FEE74 pKa = 5.79 KK75 pKa = 10.22 IMRR78 pKa = 11.84 KK79 pKa = 7.42 STEE82 pKa = 4.01 DD83 pKa = 2.98 AKK85 pKa = 10.76 KK86 pKa = 9.5 TMVSLKK92 pKa = 10.01 NKK94 pKa = 8.82 YY95 pKa = 10.22 AIVSGRR101 pKa = 11.84 PTKK104 pKa = 11.04 NEE106 pKa = 4.84 DD107 pKa = 2.7 ITLARR112 pKa = 11.84 VAATFPMITFKK123 pKa = 10.95 VVEE126 pKa = 4.2 AVKK129 pKa = 10.04 PSRR132 pKa = 11.84 PAEE135 pKa = 3.68 QWLTTKK141 pKa = 10.77 GIVITKK147 pKa = 7.41 PTLKK151 pKa = 10.09 TPGAFALLKK160 pKa = 10.73 RR161 pKa = 11.84 NMEE164 pKa = 4.25 AKK166 pKa = 10.01 KK167 pKa = 10.9 SEE169 pKa = 3.98 MDD171 pKa = 3.74 TIKK174 pKa = 10.75 CLLLIQHH181 pKa = 6.97 AEE183 pKa = 4.02 SEE185 pKa = 4.56 IINAQNQSYY194 pKa = 9.98 RR195 pKa = 11.84 SKK197 pKa = 10.99 SLEE200 pKa = 4.01 EE201 pKa = 4.07 KK202 pKa = 10.82 LQDD205 pKa = 3.62 LLLYY209 pKa = 10.78 ARR211 pKa = 11.84 AAFNNAMTSEE221 pKa = 4.6 DD222 pKa = 3.81 DD223 pKa = 4.19 RR224 pKa = 11.84 STFTNALNSDD234 pKa = 3.41 LRR236 pKa = 11.84 DD237 pKa = 3.64 VKK239 pKa = 10.82 RR240 pKa = 11.84 KK241 pKa = 9.88 LVDD244 pKa = 3.15 QFASIFGSSNDD255 pKa = 3.72 SVISASFFGSNEE267 pKa = 3.65 VEE269 pKa = 4.05 AA270 pKa = 5.56
Molecular weight: 30.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.523
IPC2_protein 8.39
IPC_protein 8.331
Toseland 9.194
ProMoST 8.858
Dawson 9.414
Bjellqvist 9.077
Wikipedia 9.545
Rodwell 9.78
Grimsley 9.458
Solomon 9.487
Lehninger 9.458
Nozaki 9.224
DTASelect 9.063
Thurlkill 9.268
EMBOSS 9.604
Sillero 9.355
Patrickios 5.219
IPC_peptide 9.487
IPC2_peptide 7.527
IPC2.peptide.svr19 7.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3836
270
2192
1278.7
145.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.353 ± 1.155
1.903 ± 0.864
5.37 ± 0.573
7.117 ± 0.565
4.745 ± 0.16
4.406 ± 0.319
2.242 ± 0.42
7.925 ± 0.259
7.195 ± 0.604
9.15 ± 0.3
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.815 ± 0.133
5.292 ± 0.315
3.597 ± 0.313
3.154 ± 0.317
4.745 ± 0.797
9.645 ± 0.243
5.865 ± 0.436
5.97 ± 0.469
0.938 ± 0.166
3.571 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here