Alysiella filiformis DSM 16848

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Alysiella; Alysiella filiformis

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286E1U2|A0A286E1U2_9NEIS Histidine kinase OS=Alysiella filiformis DSM 16848 OX=1120981 GN=SAMN02746062_00071 PE=4 SV=1
MM1 pKa = 7.44SLNIPVEE8 pKa = 4.2FQDD11 pKa = 4.4FADD14 pKa = 5.39EE15 pKa = 5.16IIASCKK21 pKa = 9.47PSVPFTLTVAPTQLWDD37 pKa = 3.71SKK39 pKa = 11.56VGGKK43 pKa = 9.63PYY45 pKa = 10.51LPRR48 pKa = 11.84DD49 pKa = 3.21FAYY52 pKa = 9.98PNNPDD57 pKa = 3.54GEE59 pKa = 4.47PLFFLAQINFAQMSALPDD77 pKa = 4.11FPSKK81 pKa = 10.98GILQFFIDD89 pKa = 5.21GKK91 pKa = 10.32DD92 pKa = 3.07DD93 pKa = 3.84CYY95 pKa = 11.38GINFDD100 pKa = 4.12NYY102 pKa = 10.53QDD104 pKa = 3.51NSHH107 pKa = 6.41YY108 pKa = 10.89KK109 pKa = 10.37LIYY112 pKa = 9.54HH113 pKa = 7.02ADD115 pKa = 3.39VVEE118 pKa = 4.66NADD121 pKa = 4.21EE122 pKa = 4.24LQQDD126 pKa = 3.65FDD128 pKa = 4.27FVQFDD133 pKa = 3.8EE134 pKa = 4.69TDD136 pKa = 3.26SGVPFPPNHH145 pKa = 6.67SYY147 pKa = 10.82QIYY150 pKa = 8.9FQAATPQPITQHH162 pKa = 6.71DD163 pKa = 3.96FAFGEE168 pKa = 4.53VVPEE172 pKa = 4.23LFDD175 pKa = 4.4FLDD178 pKa = 4.32EE179 pKa = 4.67KK180 pKa = 11.47SEE182 pKa = 4.48DD183 pKa = 5.32DD184 pKa = 3.89DD185 pKa = 5.35YY186 pKa = 12.13EE187 pKa = 4.09WDD189 pKa = 3.51LHH191 pKa = 6.74DD192 pKa = 6.0KK193 pKa = 10.95YY194 pKa = 11.6DD195 pKa = 3.65EE196 pKa = 4.43FSNSEE201 pKa = 3.61QHH203 pKa = 6.61RR204 pKa = 11.84VGGYY208 pKa = 9.83PYY210 pKa = 9.35FTQDD214 pKa = 3.09DD215 pKa = 3.85VRR217 pKa = 11.84GYY219 pKa = 11.34GSYY222 pKa = 10.8DD223 pKa = 3.17LKK225 pKa = 11.34DD226 pKa = 3.67YY227 pKa = 11.45VLLFQLVSDD236 pKa = 4.09WRR238 pKa = 11.84DD239 pKa = 3.18SNDD242 pKa = 3.99DD243 pKa = 3.78VNIMWGDD250 pKa = 3.6CGVGNFFIRR259 pKa = 11.84PEE261 pKa = 4.0DD262 pKa = 3.78LKK264 pKa = 11.57NLAFDD269 pKa = 3.79KK270 pKa = 11.31AFFTMDD276 pKa = 3.35CCC278 pKa = 5.69

Molecular weight: 32.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286EAI6|A0A286EAI6_9NEIS GTPase Era OS=Alysiella filiformis DSM 16848 OX=1120981 GN=era PE=3 SV=1
MM1 pKa = 7.63ALRR4 pKa = 11.84RR5 pKa = 11.84HH6 pKa = 4.7THH8 pKa = 7.05ALGLWHH14 pKa = 6.39ATVRR18 pKa = 11.84ALKK21 pKa = 10.4IGFQNKK27 pKa = 8.82NMLFAKK33 pKa = 9.17PRR35 pKa = 11.84LMFRR39 pKa = 11.84QNVIQMRR46 pKa = 3.81

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2283

0

2283

683258

13

2288

299.3

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.953 ± 0.069

0.969 ± 0.02

5.18 ± 0.045

5.628 ± 0.054

4.159 ± 0.039

6.693 ± 0.056

2.676 ± 0.038

6.083 ± 0.039

5.501 ± 0.048

10.151 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.572 ± 0.028

4.921 ± 0.055

4.103 ± 0.038

5.371 ± 0.052

4.566 ± 0.042

5.462 ± 0.037

5.105 ± 0.046

6.661 ± 0.055

1.338 ± 0.022

2.907 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski