Amino acid dipepetide frequency for Bacillus phage StevenHerd11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.288AlaAla: 0.288 ± 0.191
1.009AlaCys: 1.009 ± 0.358
2.594AlaAsp: 2.594 ± 0.712
3.747AlaGlu: 3.747 ± 0.919
3.027AlaPhe: 3.027 ± 0.729
3.603AlaGly: 3.603 ± 0.872
0.0AlaHis: 0.0 ± 0.0
2.45AlaIle: 2.45 ± 0.667
5.045AlaLys: 5.045 ± 0.792
2.594AlaLeu: 2.594 ± 0.477
2.018AlaMet: 2.018 ± 0.495
2.45AlaAsn: 2.45 ± 0.482
1.441AlaPro: 1.441 ± 0.589
2.162AlaGln: 2.162 ± 0.545
1.297AlaArg: 1.297 ± 0.357
2.162AlaSer: 2.162 ± 0.474
3.027AlaThr: 3.027 ± 0.626
2.739AlaVal: 2.739 ± 0.76
0.721AlaTrp: 0.721 ± 0.323
2.306AlaTyr: 2.306 ± 0.539
0.0AlaXaa: 0.0 ± 0.0
Cys
0.432CysAla: 0.432 ± 0.287
0.288CysCys: 0.288 ± 0.18
1.585CysAsp: 1.585 ± 0.497
1.297CysGlu: 1.297 ± 0.338
0.288CysPhe: 0.288 ± 0.193
0.865CysGly: 0.865 ± 0.504
0.144CysHis: 0.144 ± 0.129
0.577CysIle: 0.577 ± 0.298
1.585CysLys: 1.585 ± 0.459
0.288CysLeu: 0.288 ± 0.192
0.432CysMet: 0.432 ± 0.215
0.288CysAsn: 0.288 ± 0.209
0.721CysPro: 0.721 ± 0.372
0.144CysGln: 0.144 ± 0.15
0.432CysArg: 0.432 ± 0.251
0.432CysSer: 0.432 ± 0.215
0.0CysThr: 0.0 ± 0.0
0.577CysVal: 0.577 ± 0.371
0.288CysTrp: 0.288 ± 0.169
0.577CysTyr: 0.577 ± 0.233
0.0CysXaa: 0.0 ± 0.0
Asp
3.027AspAla: 3.027 ± 0.479
1.441AspCys: 1.441 ± 0.663
3.459AspAsp: 3.459 ± 0.682
5.189AspGlu: 5.189 ± 0.753
4.18AspPhe: 4.18 ± 0.576
5.765AspGly: 5.765 ± 1.297
1.153AspHis: 1.153 ± 0.406
5.189AspIle: 5.189 ± 0.715
6.342AspLys: 6.342 ± 0.621
4.036AspLeu: 4.036 ± 0.836
2.45AspMet: 2.45 ± 0.616
4.468AspAsn: 4.468 ± 0.899
2.45AspPro: 2.45 ± 0.56
2.162AspGln: 2.162 ± 0.438
1.874AspArg: 1.874 ± 0.538
2.162AspSer: 2.162 ± 0.676
3.315AspThr: 3.315 ± 0.58
5.477AspVal: 5.477 ± 0.964
0.288AspTrp: 0.288 ± 0.182
3.027AspTyr: 3.027 ± 0.604
0.0AspXaa: 0.0 ± 0.0
Glu
3.315GluAla: 3.315 ± 0.719
0.721GluCys: 0.721 ± 0.371
3.747GluAsp: 3.747 ± 0.758
5.621GluGlu: 5.621 ± 1.452
4.036GluPhe: 4.036 ± 0.948
4.324GluGly: 4.324 ± 0.763
0.721GluHis: 0.721 ± 0.227
6.342GluIle: 6.342 ± 1.057
6.63GluLys: 6.63 ± 1.358
9.369GluLeu: 9.369 ± 1.367
2.594GluMet: 2.594 ± 0.735
5.621GluAsn: 5.621 ± 0.891
1.009GluPro: 1.009 ± 0.43
3.315GluGln: 3.315 ± 0.575
3.747GluArg: 3.747 ± 0.957
4.756GluSer: 4.756 ± 0.756
4.468GluThr: 4.468 ± 0.897
5.045GluVal: 5.045 ± 0.942
1.009GluTrp: 1.009 ± 0.304
3.459GluTyr: 3.459 ± 0.71
0.0GluXaa: 0.0 ± 0.0
Phe
1.874PheAla: 1.874 ± 0.437
0.432PheCys: 0.432 ± 0.254
3.892PheAsp: 3.892 ± 0.593
4.324PheGlu: 4.324 ± 0.667
1.73PhePhe: 1.73 ± 0.437
2.45PheGly: 2.45 ± 0.713
1.441PheHis: 1.441 ± 0.455
4.18PheIle: 4.18 ± 0.721
5.189PheLys: 5.189 ± 1.083
2.594PheLeu: 2.594 ± 0.487
2.162PheMet: 2.162 ± 0.543
2.45PheAsn: 2.45 ± 0.839
0.865PhePro: 0.865 ± 0.372
1.009PheGln: 1.009 ± 0.437
0.865PheArg: 0.865 ± 0.363
4.18PheSer: 4.18 ± 0.943
2.306PheThr: 2.306 ± 0.686
2.594PheVal: 2.594 ± 0.61
0.432PheTrp: 0.432 ± 0.217
2.306PheTyr: 2.306 ± 0.669
0.0PheXaa: 0.0 ± 0.0
Gly
3.027GlyAla: 3.027 ± 1.034
0.288GlyCys: 0.288 ± 0.226
2.883GlyAsp: 2.883 ± 0.958
6.054GlyGlu: 6.054 ± 0.802
3.171GlyPhe: 3.171 ± 0.556
4.036GlyGly: 4.036 ± 0.958
0.865GlyHis: 0.865 ± 0.35
4.901GlyIle: 4.901 ± 0.995
6.342GlyLys: 6.342 ± 1.283
4.901GlyLeu: 4.901 ± 0.712
2.45GlyMet: 2.45 ± 0.646
4.756GlyAsn: 4.756 ± 1.073
0.288GlyPro: 0.288 ± 0.161
2.018GlyGln: 2.018 ± 0.541
2.018GlyArg: 2.018 ± 0.552
4.18GlySer: 4.18 ± 0.789
6.054GlyThr: 6.054 ± 1.224
3.459GlyVal: 3.459 ± 0.66
0.288GlyTrp: 0.288 ± 0.191
3.027GlyTyr: 3.027 ± 0.699
0.0GlyXaa: 0.0 ± 0.0
His
0.721HisAla: 0.721 ± 0.217
0.144HisCys: 0.144 ± 0.141
1.297HisAsp: 1.297 ± 0.497
0.432HisGlu: 0.432 ± 0.256
0.721HisPhe: 0.721 ± 0.285
0.432HisGly: 0.432 ± 0.215
0.577HisHis: 0.577 ± 0.324
1.874HisIle: 1.874 ± 0.436
0.865HisLys: 0.865 ± 0.333
1.441HisLeu: 1.441 ± 0.494
0.288HisMet: 0.288 ± 0.171
0.721HisAsn: 0.721 ± 0.27
0.0HisPro: 0.0 ± 0.0
0.432HisGln: 0.432 ± 0.216
0.577HisArg: 0.577 ± 0.249
0.432HisSer: 0.432 ± 0.243
1.585HisThr: 1.585 ± 0.415
1.297HisVal: 1.297 ± 0.509
0.144HisTrp: 0.144 ± 0.16
0.865HisTyr: 0.865 ± 0.328
0.0HisXaa: 0.0 ± 0.0
Ile
3.892IleAla: 3.892 ± 0.704
0.288IleCys: 0.288 ± 0.19
6.342IleAsp: 6.342 ± 0.74
6.342IleGlu: 6.342 ± 1.219
2.162IlePhe: 2.162 ± 0.444
4.612IleGly: 4.612 ± 0.696
1.009IleHis: 1.009 ± 0.415
4.324IleIle: 4.324 ± 1.094
6.918IleLys: 6.918 ± 0.914
2.018IleLeu: 2.018 ± 0.724
2.306IleMet: 2.306 ± 0.528
5.477IleAsn: 5.477 ± 0.777
1.441IlePro: 1.441 ± 0.388
2.45IleGln: 2.45 ± 0.439
3.603IleArg: 3.603 ± 0.781
3.027IleSer: 3.027 ± 0.542
4.756IleThr: 4.756 ± 0.826
3.747IleVal: 3.747 ± 0.721
0.865IleTrp: 0.865 ± 0.307
3.171IleTyr: 3.171 ± 0.686
0.0IleXaa: 0.0 ± 0.0
Lys
4.612LysAla: 4.612 ± 1.001
1.297LysCys: 1.297 ± 0.477
7.639LysAsp: 7.639 ± 1.186
8.936LysGlu: 8.936 ± 1.552
4.468LysPhe: 4.468 ± 0.897
6.774LysGly: 6.774 ± 0.941
1.441LysHis: 1.441 ± 0.429
6.342LysIle: 6.342 ± 0.735
11.098LysLys: 11.098 ± 1.337
6.63LysLeu: 6.63 ± 0.783
3.603LysMet: 3.603 ± 0.704
4.324LysAsn: 4.324 ± 0.723
1.297LysPro: 1.297 ± 0.443
3.747LysGln: 3.747 ± 1.019
3.603LysArg: 3.603 ± 0.616
3.892LysSer: 3.892 ± 0.812
5.045LysThr: 5.045 ± 0.929
6.63LysVal: 6.63 ± 0.751
1.009LysTrp: 1.009 ± 0.444
4.612LysTyr: 4.612 ± 0.801
0.0LysXaa: 0.0 ± 0.0
Leu
2.883LeuAla: 2.883 ± 0.557
0.144LeuCys: 0.144 ± 0.157
5.621LeuAsp: 5.621 ± 0.816
5.621LeuGlu: 5.621 ± 1.111
2.306LeuPhe: 2.306 ± 0.57
4.324LeuGly: 4.324 ± 0.944
1.441LeuHis: 1.441 ± 0.412
3.603LeuIle: 3.603 ± 0.622
8.071LeuLys: 8.071 ± 1.357
3.892LeuLeu: 3.892 ± 0.662
1.874LeuMet: 1.874 ± 0.47
4.18LeuAsn: 4.18 ± 0.617
2.306LeuPro: 2.306 ± 0.449
3.315LeuGln: 3.315 ± 0.831
3.027LeuArg: 3.027 ± 0.705
3.603LeuSer: 3.603 ± 0.695
4.18LeuThr: 4.18 ± 0.623
3.747LeuVal: 3.747 ± 0.637
0.865LeuTrp: 0.865 ± 0.359
2.883LeuTyr: 2.883 ± 0.532
0.0LeuXaa: 0.0 ± 0.0
Met
1.009MetAla: 1.009 ± 0.276
1.009MetCys: 1.009 ± 0.399
2.162MetAsp: 2.162 ± 0.535
1.73MetGlu: 1.73 ± 0.482
2.162MetPhe: 2.162 ± 0.506
1.874MetGly: 1.874 ± 0.448
0.144MetHis: 0.144 ± 0.141
2.018MetIle: 2.018 ± 0.585
2.306MetLys: 2.306 ± 0.561
2.162MetLeu: 2.162 ± 0.606
1.441MetMet: 1.441 ± 0.507
3.459MetAsn: 3.459 ± 0.658
0.577MetPro: 0.577 ± 0.275
1.441MetGln: 1.441 ± 0.356
1.297MetArg: 1.297 ± 0.332
2.45MetSer: 2.45 ± 0.576
1.585MetThr: 1.585 ± 0.454
1.297MetVal: 1.297 ± 0.354
1.297MetTrp: 1.297 ± 0.377
1.441MetTyr: 1.441 ± 0.395
0.0MetXaa: 0.0 ± 0.0
Asn
2.739AsnAla: 2.739 ± 0.756
0.721AsnCys: 0.721 ± 0.369
4.756AsnAsp: 4.756 ± 0.603
4.468AsnGlu: 4.468 ± 0.714
3.747AsnPhe: 3.747 ± 0.745
4.324AsnGly: 4.324 ± 0.909
0.865AsnHis: 0.865 ± 0.311
4.756AsnIle: 4.756 ± 0.64
5.621AsnLys: 5.621 ± 0.889
4.324AsnLeu: 4.324 ± 0.633
1.297AsnMet: 1.297 ± 0.44
6.486AsnAsn: 6.486 ± 1.305
2.594AsnPro: 2.594 ± 0.59
2.739AsnGln: 2.739 ± 0.595
3.171AsnArg: 3.171 ± 0.917
4.612AsnSer: 4.612 ± 0.747
3.171AsnThr: 3.171 ± 1.174
4.756AsnVal: 4.756 ± 0.926
0.721AsnTrp: 0.721 ± 0.266
3.027AsnTyr: 3.027 ± 0.615
0.0AsnXaa: 0.0 ± 0.0
Pro
0.865ProAla: 0.865 ± 0.347
0.288ProCys: 0.288 ± 0.176
1.585ProAsp: 1.585 ± 0.443
2.162ProGlu: 2.162 ± 0.55
1.297ProPhe: 1.297 ± 0.498
0.865ProGly: 0.865 ± 0.304
0.288ProHis: 0.288 ± 0.2
1.585ProIle: 1.585 ± 0.424
1.874ProLys: 1.874 ± 0.561
1.297ProLeu: 1.297 ± 0.377
1.009ProMet: 1.009 ± 0.454
1.73ProAsn: 1.73 ± 0.604
0.865ProPro: 0.865 ± 0.23
0.577ProGln: 0.577 ± 0.28
0.721ProArg: 0.721 ± 0.337
1.585ProSer: 1.585 ± 0.603
1.874ProThr: 1.874 ± 0.522
2.162ProVal: 2.162 ± 0.637
0.288ProTrp: 0.288 ± 0.178
1.441ProTyr: 1.441 ± 0.572
0.0ProXaa: 0.0 ± 0.0
Gln
1.009GlnAla: 1.009 ± 0.319
0.144GlnCys: 0.144 ± 0.129
1.874GlnAsp: 1.874 ± 0.552
1.585GlnGlu: 1.585 ± 0.623
1.153GlnPhe: 1.153 ± 0.449
2.162GlnGly: 2.162 ± 0.807
0.432GlnHis: 0.432 ± 0.224
1.874GlnIle: 1.874 ± 0.541
3.171GlnLys: 3.171 ± 0.758
4.756GlnLeu: 4.756 ± 0.918
0.432GlnMet: 0.432 ± 0.247
3.171GlnAsn: 3.171 ± 0.504
1.153GlnPro: 1.153 ± 0.378
0.721GlnGln: 0.721 ± 0.26
1.874GlnArg: 1.874 ± 0.612
1.585GlnSer: 1.585 ± 0.335
2.45GlnThr: 2.45 ± 0.53
2.018GlnVal: 2.018 ± 0.498
0.288GlnTrp: 0.288 ± 0.19
2.739GlnTyr: 2.739 ± 0.588
0.0GlnXaa: 0.0 ± 0.0
Arg
3.171ArgAla: 3.171 ± 0.582
0.288ArgCys: 0.288 ± 0.221
2.45ArgAsp: 2.45 ± 0.595
3.892ArgGlu: 3.892 ± 1.012
2.306ArgPhe: 2.306 ± 0.474
2.306ArgGly: 2.306 ± 0.831
0.577ArgHis: 0.577 ± 0.273
1.73ArgIle: 1.73 ± 0.455
4.324ArgLys: 4.324 ± 0.802
2.018ArgLeu: 2.018 ± 0.447
1.297ArgMet: 1.297 ± 0.451
1.874ArgAsn: 1.874 ± 0.528
0.865ArgPro: 0.865 ± 0.371
1.585ArgGln: 1.585 ± 0.47
2.45ArgArg: 2.45 ± 0.644
1.73ArgSer: 1.73 ± 0.33
2.018ArgThr: 2.018 ± 0.615
1.874ArgVal: 1.874 ± 0.794
0.288ArgTrp: 0.288 ± 0.174
2.594ArgTyr: 2.594 ± 0.74
0.0ArgXaa: 0.0 ± 0.0
Ser
2.594SerAla: 2.594 ± 0.515
0.721SerCys: 0.721 ± 0.309
3.171SerAsp: 3.171 ± 0.63
3.747SerGlu: 3.747 ± 0.787
2.594SerPhe: 2.594 ± 0.549
3.171SerGly: 3.171 ± 0.7
1.153SerHis: 1.153 ± 0.287
3.603SerIle: 3.603 ± 0.685
4.18SerLys: 4.18 ± 0.762
4.756SerLeu: 4.756 ± 0.874
1.585SerMet: 1.585 ± 0.603
4.468SerAsn: 4.468 ± 0.789
0.865SerPro: 0.865 ± 0.335
1.874SerGln: 1.874 ± 0.537
2.306SerArg: 2.306 ± 0.639
2.594SerSer: 2.594 ± 0.7
2.883SerThr: 2.883 ± 0.635
3.171SerVal: 3.171 ± 0.698
0.288SerTrp: 0.288 ± 0.194
2.883SerTyr: 2.883 ± 0.687
0.0SerXaa: 0.0 ± 0.0
Thr
3.315ThrAla: 3.315 ± 0.877
0.721ThrCys: 0.721 ± 0.352
3.171ThrAsp: 3.171 ± 0.787
4.18ThrGlu: 4.18 ± 0.874
2.45ThrPhe: 2.45 ± 0.412
4.756ThrGly: 4.756 ± 1.075
0.577ThrHis: 0.577 ± 0.292
6.198ThrIle: 6.198 ± 0.782
4.901ThrLys: 4.901 ± 0.902
4.901ThrLeu: 4.901 ± 1.084
2.162ThrMet: 2.162 ± 0.437
3.892ThrAsn: 3.892 ± 0.814
1.585ThrPro: 1.585 ± 0.318
1.297ThrGln: 1.297 ± 0.435
1.874ThrArg: 1.874 ± 0.41
3.603ThrSer: 3.603 ± 0.846
4.036ThrThr: 4.036 ± 1.328
4.18ThrVal: 4.18 ± 0.777
0.288ThrTrp: 0.288 ± 0.21
1.585ThrTyr: 1.585 ± 0.463
0.0ThrXaa: 0.0 ± 0.0
Val
2.45ValAla: 2.45 ± 0.597
0.144ValCys: 0.144 ± 0.139
5.333ValAsp: 5.333 ± 0.911
5.765ValGlu: 5.765 ± 0.821
2.306ValPhe: 2.306 ± 0.585
5.189ValGly: 5.189 ± 0.621
1.009ValHis: 1.009 ± 0.424
3.747ValIle: 3.747 ± 0.784
6.342ValLys: 6.342 ± 0.708
3.315ValLeu: 3.315 ± 0.58
1.585ValMet: 1.585 ± 0.482
3.315ValAsn: 3.315 ± 0.655
2.883ValPro: 2.883 ± 0.731
1.585ValGln: 1.585 ± 0.466
2.306ValArg: 2.306 ± 0.504
3.171ValSer: 3.171 ± 0.524
4.18ValThr: 4.18 ± 0.902
4.612ValVal: 4.612 ± 1.093
1.009ValTrp: 1.009 ± 0.347
3.027ValTyr: 3.027 ± 0.699
0.0ValXaa: 0.0 ± 0.0
Trp
0.144TrpAla: 0.144 ± 0.15
0.144TrpCys: 0.144 ± 0.123
0.432TrpAsp: 0.432 ± 0.295
0.577TrpGlu: 0.577 ± 0.301
1.585TrpPhe: 1.585 ± 0.442
0.577TrpGly: 0.577 ± 0.271
0.577TrpHis: 0.577 ± 0.23
0.288TrpIle: 0.288 ± 0.189
1.009TrpLys: 1.009 ± 0.337
0.865TrpLeu: 0.865 ± 0.404
0.577TrpMet: 0.577 ± 0.239
1.585TrpAsn: 1.585 ± 0.415
0.0TrpPro: 0.0 ± 0.0
0.577TrpGln: 0.577 ± 0.239
0.721TrpArg: 0.721 ± 0.327
0.432TrpSer: 0.432 ± 0.282
0.288TrpThr: 0.288 ± 0.204
0.721TrpVal: 0.721 ± 0.308
0.288TrpTrp: 0.288 ± 0.221
0.577TrpTyr: 0.577 ± 0.292
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.315TyrAla: 3.315 ± 0.613
1.009TyrCys: 1.009 ± 0.342
3.459TyrAsp: 3.459 ± 0.621
4.18TyrGlu: 4.18 ± 0.773
1.585TyrPhe: 1.585 ± 0.362
2.739TyrGly: 2.739 ± 0.581
0.432TyrHis: 0.432 ± 0.207
3.171TyrIle: 3.171 ± 0.733
5.189TyrLys: 5.189 ± 0.778
1.73TyrLeu: 1.73 ± 0.511
1.153TyrMet: 1.153 ± 0.551
4.18TyrAsn: 4.18 ± 0.597
1.153TyrPro: 1.153 ± 0.351
1.441TyrGln: 1.441 ± 0.394
1.874TyrArg: 1.874 ± 0.472
2.018TyrSer: 2.018 ± 0.468
2.45TyrThr: 2.45 ± 0.457
3.171TyrVal: 3.171 ± 0.654
1.297TyrTrp: 1.297 ± 0.571
2.594TyrTyr: 2.594 ± 0.709
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 37 proteins (6939 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski