Vagococcus entomophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430AGJ2|A0A430AGJ2_9ENTE Probable transcriptional regulatory protein CBF30_07250 OS=Vagococcus entomophilus OX=1160095 GN=CBF30_07250 PE=3 SV=1
MM1 pKa = 7.17AHH3 pKa = 6.05EE4 pKa = 4.36HH5 pKa = 6.16HH6 pKa = 7.21HH7 pKa = 6.51DD8 pKa = 3.54HH9 pKa = 6.87DD10 pKa = 4.94HH11 pKa = 5.2EE12 pKa = 5.84HH13 pKa = 5.39EE14 pKa = 5.54HH15 pKa = 5.91ITLVDD20 pKa = 3.67DD21 pKa = 4.43QGNEE25 pKa = 3.98TLFEE29 pKa = 4.24ILLTIDD35 pKa = 3.35GQEE38 pKa = 4.2EE39 pKa = 4.17FGKK42 pKa = 10.54SYY44 pKa = 11.38VLLYY48 pKa = 7.54PTGVSDD54 pKa = 4.63DD55 pKa = 4.48EE56 pKa = 4.42EE57 pKa = 5.27VEE59 pKa = 4.22LQAYY63 pKa = 9.78AYY65 pKa = 9.68EE66 pKa = 4.09EE67 pKa = 4.52AEE69 pKa = 4.12NGSEE73 pKa = 4.41GEE75 pKa = 4.12LKK77 pKa = 10.42EE78 pKa = 4.13IQTDD82 pKa = 4.0AEE84 pKa = 3.73WDD86 pKa = 3.62MIEE89 pKa = 4.38EE90 pKa = 4.22VFNTFMAEE98 pKa = 4.02EE99 pKa = 3.96EE100 pKa = 4.43AEE102 pKa = 4.09

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430AGA6|A0A430AGA6_9ENTE Pseudouridine synthase OS=Vagococcus entomophilus OX=1160095 GN=CBF30_06660 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.01NGRR28 pKa = 11.84RR29 pKa = 11.84VVAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.2GRR39 pKa = 11.84KK40 pKa = 9.06VISAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2255

0

2255

702968

32

1819

311.7

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.79 ± 0.055

0.781 ± 0.017

4.759 ± 0.041

7.15 ± 0.063

4.625 ± 0.047

6.372 ± 0.047

1.868 ± 0.022

7.554 ± 0.05

7.828 ± 0.047

9.933 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.549 ± 0.026

4.698 ± 0.038

3.26 ± 0.025

4.456 ± 0.045

3.512 ± 0.032

6.438 ± 0.048

5.939 ± 0.044

6.902 ± 0.045

0.863 ± 0.019

3.723 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski