Streptococcus phage Javan73

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AQP2|A0A4D6AQP2_9CAUD Transcriptional regulator OS=Streptococcus phage Javan73 OX=2548304 GN=Javan73_0028 PE=4 SV=1
MM1 pKa = 7.06MSKK4 pKa = 10.31FINADD9 pKa = 3.37CLNVMRR15 pKa = 11.84QYY17 pKa = 11.19PDD19 pKa = 4.33DD20 pKa = 4.41YY21 pKa = 11.28FDD23 pKa = 4.78LAIVDD28 pKa = 4.0PPYY31 pKa = 10.69FSGPEE36 pKa = 3.76KK37 pKa = 10.86

Molecular weight: 4.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ALW7|A0A4D6ALW7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan73 OX=2548304 GN=Javan73_0024 PE=4 SV=1
MM1 pKa = 7.57NNLQIIAVGTIVSVVLIEE19 pKa = 4.02SLIMNVKK26 pKa = 9.89LKK28 pKa = 10.57KK29 pKa = 10.19ALRR32 pKa = 11.84AKK34 pKa = 10.37KK35 pKa = 8.38STRR38 pKa = 11.84TVMTHH43 pKa = 6.66PAAQRR48 pKa = 11.84GLIDD52 pKa = 3.69YY53 pKa = 7.52KK54 pKa = 9.7TGRR57 pKa = 11.84RR58 pKa = 11.84VDD60 pKa = 3.57INPKK64 pKa = 6.32TRR66 pKa = 11.84KK67 pKa = 9.24EE68 pKa = 4.27VFVDD72 pKa = 3.68

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

9902

37

1022

180.0

20.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.332 ± 0.421

0.606 ± 0.116

6.14 ± 0.395

7.726 ± 0.472

4.383 ± 0.311

5.878 ± 0.31

1.606 ± 0.18

7.544 ± 0.439

8.534 ± 0.382

8.574 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.797 ± 0.217

6.13 ± 0.257

2.646 ± 0.153

3.979 ± 0.157

4.08 ± 0.324

6.08 ± 0.371

5.898 ± 0.378

6.11 ± 0.236

1.131 ± 0.16

3.828 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski