Tortoise microvirus 104
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6Z6|A0A4P8W6Z6_9VIRU Uncharacterized protein OS=Tortoise microvirus 104 OX=2583104 PE=4 SV=1
MM1 pKa = 7.65 HH2 pKa = 7.03 NNIYY6 pKa = 10.22 IIYY9 pKa = 8.43 NVLSKK14 pKa = 10.76 RR15 pKa = 11.84 YY16 pKa = 8.99 GDD18 pKa = 3.6 VVAYY22 pKa = 9.65 PSDD25 pKa = 3.22 AFAARR30 pKa = 11.84 RR31 pKa = 11.84 LSEE34 pKa = 4.12 VYY36 pKa = 9.97 KK37 pKa = 10.48 DD38 pKa = 3.69 HH39 pKa = 7.66 PEE41 pKa = 3.89 HH42 pKa = 6.2 LKK44 pKa = 10.86 EE45 pKa = 4.45 MEE47 pKa = 4.02 LCRR50 pKa = 11.84 IGSVSIEE57 pKa = 4.19 TGVVTPHH64 pKa = 6.61 DD65 pKa = 5.19 PIRR68 pKa = 11.84 IDD70 pKa = 4.65 LDD72 pKa = 3.55 VDD74 pKa = 3.64 PDD76 pKa = 4.01 SPLSLNEE83 pKa = 3.74
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.812
IPC2_protein 4.94
IPC_protein 4.813
Toseland 4.749
ProMoST 4.825
Dawson 4.825
Bjellqvist 5.016
Wikipedia 4.724
Rodwell 4.724
Grimsley 4.673
Solomon 4.825
Lehninger 4.774
Nozaki 4.952
DTASelect 5.143
Thurlkill 4.762
EMBOSS 4.774
Sillero 5.003
Patrickios 3.605
IPC_peptide 4.825
IPC2_peptide 5.003
IPC2.peptide.svr19 4.912
Protein with the highest isoelectric point:
>tr|A0A4V1FW28|A0A4V1FW28_9VIRU Uncharacterized protein OS=Tortoise microvirus 104 OX=2583104 PE=4 SV=1
MM1 pKa = 7.64 GNLCSIRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 10.35 LSITRR15 pKa = 11.84 QHH17 pKa = 6.17 TPQSINYY24 pKa = 7.6 QLQYY28 pKa = 10.95 SEE30 pKa = 4.72 TVSHH34 pKa = 6.63 KK35 pKa = 10.3 HH36 pKa = 5.69 PKK38 pKa = 9.01 LAFWVQLPVLPCRR51 pKa = 11.84 AKK53 pKa = 9.84 RR54 pKa = 11.84 EE55 pKa = 4.24 KK56 pKa = 10.92 KK57 pKa = 10.14 NFHH60 pKa = 5.8 LTHH63 pKa = 5.77 VKK65 pKa = 10.23 KK66 pKa = 10.94 SNKK69 pKa = 9.67 NYY71 pKa = 8.51 PQSNKK76 pKa = 10.09 KK77 pKa = 8.98 MLTTFFICVILILGGWHH94 pKa = 7.3 DD95 pKa = 5.05 LYY97 pKa = 11.51 NAA99 pKa = 4.33
Molecular weight: 11.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.663
IPC_protein 9.692
Toseland 10.438
ProMoST 9.999
Dawson 10.555
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 11.199
Grimsley 10.599
Solomon 10.584
Lehninger 10.57
Nozaki 10.438
DTASelect 10.175
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.467
Patrickios 10.935
IPC_peptide 10.584
IPC2_peptide 9.004
IPC2.peptide.svr19 8.541
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1720
67
572
191.1
21.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.57 ± 0.94
1.279 ± 0.413
6.163 ± 0.615
4.826 ± 0.944
3.779 ± 0.782
4.942 ± 0.532
2.093 ± 0.454
6.395 ± 0.585
6.744 ± 1.464
8.779 ± 0.469
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.674 ± 0.365
5.174 ± 0.394
4.244 ± 0.748
6.279 ± 1.396
4.709 ± 0.232
7.733 ± 1.002
6.744 ± 0.748
4.36 ± 0.464
0.872 ± 0.148
5.64 ± 0.621
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here