Amino acid dipepetide frequency for Klebsiella phage N1M2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.776AlaAla: 4.776 ± 0.373
0.474AlaCys: 0.474 ± 0.076
3.828AlaAsp: 3.828 ± 0.211
3.687AlaGlu: 3.687 ± 0.214
2.612AlaPhe: 2.612 ± 0.201
4.469AlaGly: 4.469 ± 0.272
1.024AlaHis: 1.024 ± 0.127
4.456AlaIle: 4.456 ± 0.319
4.366AlaLys: 4.366 ± 0.25
4.814AlaLeu: 4.814 ± 0.265
1.831AlaMet: 1.831 ± 0.177
3.393AlaAsn: 3.393 ± 0.244
2.138AlaPro: 2.138 ± 0.241
2.01AlaGln: 2.01 ± 0.193
2.638AlaArg: 2.638 ± 0.234
3.508AlaSer: 3.508 ± 0.229
3.931AlaThr: 3.931 ± 0.227
4.148AlaVal: 4.148 ± 0.238
0.691AlaTrp: 0.691 ± 0.095
2.023AlaTyr: 2.023 ± 0.176
0.0AlaXaa: 0.0 ± 0.0
Cys
0.32CysAla: 0.32 ± 0.069
0.077CysCys: 0.077 ± 0.029
0.525CysAsp: 0.525 ± 0.075
0.487CysGlu: 0.487 ± 0.076
0.359CysPhe: 0.359 ± 0.07
0.563CysGly: 0.563 ± 0.088
0.192CysHis: 0.192 ± 0.053
0.615CysIle: 0.615 ± 0.092
0.615CysLys: 0.615 ± 0.087
0.704CysLeu: 0.704 ± 0.095
0.166CysMet: 0.166 ± 0.044
0.359CysAsn: 0.359 ± 0.064
0.371CysPro: 0.371 ± 0.068
0.282CysGln: 0.282 ± 0.067
0.64CysArg: 0.64 ± 0.091
0.627CysSer: 0.627 ± 0.099
0.346CysThr: 0.346 ± 0.058
0.615CysVal: 0.615 ± 0.094
0.166CysTrp: 0.166 ± 0.041
0.499CysTyr: 0.499 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
3.905AspAla: 3.905 ± 0.246
0.538AspCys: 0.538 ± 0.085
3.559AspAsp: 3.559 ± 0.261
3.598AspGlu: 3.598 ± 0.23
3.38AspPhe: 3.38 ± 0.189
4.609AspGly: 4.609 ± 0.255
1.011AspHis: 1.011 ± 0.105
5.301AspIle: 5.301 ± 0.273
4.379AspLys: 4.379 ± 0.195
5.967AspLeu: 5.967 ± 0.287
1.664AspMet: 1.664 ± 0.142
3.572AspAsn: 3.572 ± 0.234
3.355AspPro: 3.355 ± 0.203
2.215AspGln: 2.215 ± 0.175
2.766AspArg: 2.766 ± 0.226
3.38AspSer: 3.38 ± 0.224
3.611AspThr: 3.611 ± 0.211
4.443AspVal: 4.443 ± 0.321
0.999AspTrp: 0.999 ± 0.121
2.919AspTyr: 2.919 ± 0.189
0.0AspXaa: 0.0 ± 0.0
Glu
4.161GluAla: 4.161 ± 0.28
0.666GluCys: 0.666 ± 0.105
4.072GluAsp: 4.072 ± 0.224
4.417GluGlu: 4.417 ± 0.36
2.727GluPhe: 2.727 ± 0.178
4.328GluGly: 4.328 ± 0.262
1.46GluHis: 1.46 ± 0.168
4.584GluIle: 4.584 ± 0.279
3.777GluLys: 3.777 ± 0.24
6.453GluLeu: 6.453 ± 0.344
2.036GluMet: 2.036 ± 0.17
2.906GluAsn: 2.906 ± 0.187
2.164GluPro: 2.164 ± 0.225
2.535GluGln: 2.535 ± 0.189
2.612GluArg: 2.612 ± 0.235
3.316GluSer: 3.316 ± 0.202
4.161GluThr: 4.161 ± 0.215
4.661GluVal: 4.661 ± 0.285
0.896GluTrp: 0.896 ± 0.11
2.842GluTyr: 2.842 ± 0.221
0.0GluXaa: 0.0 ± 0.0
Phe
2.228PheAla: 2.228 ± 0.177
0.282PheCys: 0.282 ± 0.057
3.303PheAsp: 3.303 ± 0.188
2.65PheGlu: 2.65 ± 0.196
1.6PhePhe: 1.6 ± 0.137
2.868PheGly: 2.868 ± 0.17
0.64PheHis: 0.64 ± 0.097
3.559PheIle: 3.559 ± 0.23
4.046PheLys: 4.046 ± 0.242
2.548PheLeu: 2.548 ± 0.204
1.37PheMet: 1.37 ± 0.165
3.675PheAsn: 3.675 ± 0.315
1.37PhePro: 1.37 ± 0.109
1.255PheGln: 1.255 ± 0.154
1.818PheArg: 1.818 ± 0.15
2.676PheSer: 2.676 ± 0.227
3.316PheThr: 3.316 ± 0.206
2.51PheVal: 2.51 ± 0.161
0.499PheTrp: 0.499 ± 0.083
1.703PheTyr: 1.703 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
3.585GlyAla: 3.585 ± 0.305
0.845GlyCys: 0.845 ± 0.128
4.264GlyAsp: 4.264 ± 0.253
4.264GlyGlu: 4.264 ± 0.223
2.996GlyPhe: 2.996 ± 0.198
4.353GlyGly: 4.353 ± 0.346
1.101GlyHis: 1.101 ± 0.116
4.814GlyIle: 4.814 ± 0.218
5.173GlyLys: 5.173 ± 0.268
5.531GlyLeu: 5.531 ± 0.263
2.1GlyMet: 2.1 ± 0.244
3.79GlyAsn: 3.79 ± 0.257
1.703GlyPro: 1.703 ± 0.184
2.113GlyGln: 2.113 ± 0.168
2.791GlyArg: 2.791 ± 0.194
3.739GlySer: 3.739 ± 0.251
4.046GlyThr: 4.046 ± 0.245
4.699GlyVal: 4.699 ± 0.276
1.05GlyTrp: 1.05 ± 0.141
3.099GlyTyr: 3.099 ± 0.188
0.0GlyXaa: 0.0 ± 0.0
His
1.152HisAla: 1.152 ± 0.135
0.32HisCys: 0.32 ± 0.059
1.165HisAsp: 1.165 ± 0.113
0.845HisGlu: 0.845 ± 0.113
0.858HisPhe: 0.858 ± 0.12
0.896HisGly: 0.896 ± 0.123
0.282HisHis: 0.282 ± 0.069
1.229HisIle: 1.229 ± 0.157
1.024HisLys: 1.024 ± 0.12
1.716HisLeu: 1.716 ± 0.15
0.384HisMet: 0.384 ± 0.073
0.883HisAsn: 0.883 ± 0.126
1.011HisPro: 1.011 ± 0.122
0.755HisGln: 0.755 ± 0.107
0.883HisArg: 0.883 ± 0.105
0.96HisSer: 0.96 ± 0.115
0.999HisThr: 0.999 ± 0.111
1.229HisVal: 1.229 ± 0.135
0.256HisTrp: 0.256 ± 0.057
0.922HisTyr: 0.922 ± 0.12
0.0HisXaa: 0.0 ± 0.0
Ile
4.225IleAla: 4.225 ± 0.287
0.615IleCys: 0.615 ± 0.099
5.493IleAsp: 5.493 ± 0.32
5.173IleGlu: 5.173 ± 0.262
2.023IlePhe: 2.023 ± 0.176
4.033IleGly: 4.033 ± 0.258
1.434IleHis: 1.434 ± 0.164
4.891IleIle: 4.891 ± 0.317
5.506IleLys: 5.506 ± 0.247
4.52IleLeu: 4.52 ± 0.246
1.6IleMet: 1.6 ± 0.151
4.545IleAsn: 4.545 ± 0.194
3.47IlePro: 3.47 ± 0.232
2.292IleGln: 2.292 ± 0.178
3.521IleArg: 3.521 ± 0.234
4.763IleSer: 4.763 ± 0.235
5.314IleThr: 5.314 ± 0.423
4.148IleVal: 4.148 ± 0.277
0.691IleTrp: 0.691 ± 0.098
2.522IleTyr: 2.522 ± 0.187
0.0IleXaa: 0.0 ± 0.0
Lys
4.494LysAla: 4.494 ± 0.28
0.525LysCys: 0.525 ± 0.077
4.968LysAsp: 4.968 ± 0.267
5.57LysGlu: 5.57 ± 0.293
3.099LysPhe: 3.099 ± 0.189
4.302LysGly: 4.302 ± 0.223
1.344LysHis: 1.344 ± 0.139
4.174LysIle: 4.174 ± 0.248
3.38LysLys: 3.38 ± 0.23
6.837LysLeu: 6.837 ± 0.328
2.1LysMet: 2.1 ± 0.175
3.265LysAsn: 3.265 ± 0.24
3.15LysPro: 3.15 ± 0.211
2.317LysGln: 2.317 ± 0.171
2.817LysArg: 2.817 ± 0.184
3.483LysSer: 3.483 ± 0.193
3.816LysThr: 3.816 ± 0.205
5.07LysVal: 5.07 ± 0.279
0.845LysTrp: 0.845 ± 0.13
2.714LysTyr: 2.714 ± 0.198
0.0LysXaa: 0.0 ± 0.0
Leu
5.314LeuAla: 5.314 ± 0.3
0.807LeuCys: 0.807 ± 0.109
5.557LeuAsp: 5.557 ± 0.293
5.416LeuGlu: 5.416 ± 0.295
2.983LeuPhe: 2.983 ± 0.23
5.083LeuGly: 5.083 ± 0.3
1.383LeuHis: 1.383 ± 0.146
5.07LeuIle: 5.07 ± 0.283
5.813LeuLys: 5.813 ± 0.274
5.864LeuLeu: 5.864 ± 0.357
2.407LeuMet: 2.407 ± 0.203
5.198LeuAsn: 5.198 ± 0.246
3.278LeuPro: 3.278 ± 0.197
2.548LeuGln: 2.548 ± 0.174
4.072LeuArg: 4.072 ± 0.246
5.275LeuSer: 5.275 ± 0.249
5.71LeuThr: 5.71 ± 0.322
6.069LeuVal: 6.069 ± 0.291
0.487LeuTrp: 0.487 ± 0.081
3.15LeuTyr: 3.15 ± 0.217
0.0LeuXaa: 0.0 ± 0.0
Met
1.729MetAla: 1.729 ± 0.155
0.23MetCys: 0.23 ± 0.057
2.01MetAsp: 2.01 ± 0.157
2.036MetGlu: 2.036 ± 0.172
1.729MetPhe: 1.729 ± 0.325
1.6MetGly: 1.6 ± 0.144
0.32MetHis: 0.32 ± 0.061
1.562MetIle: 1.562 ± 0.153
1.933MetLys: 1.933 ± 0.157
2.305MetLeu: 2.305 ± 0.214
0.858MetMet: 0.858 ± 0.118
1.831MetAsn: 1.831 ± 0.172
1.255MetPro: 1.255 ± 0.132
0.947MetGln: 0.947 ± 0.116
0.807MetArg: 0.807 ± 0.123
2.241MetSer: 2.241 ± 0.198
1.664MetThr: 1.664 ± 0.151
1.933MetVal: 1.933 ± 0.17
0.32MetTrp: 0.32 ± 0.055
0.845MetTyr: 0.845 ± 0.101
0.0MetXaa: 0.0 ± 0.0
Asn
3.278AsnAla: 3.278 ± 0.223
0.307AsnCys: 0.307 ± 0.082
3.086AsnAsp: 3.086 ± 0.215
3.393AsnGlu: 3.393 ± 0.198
2.561AsnPhe: 2.561 ± 0.214
4.507AsnGly: 4.507 ± 0.25
0.845AsnHis: 0.845 ± 0.103
4.161AsnIle: 4.161 ± 0.232
4.366AsnLys: 4.366 ± 0.21
4.827AsnLeu: 4.827 ± 0.281
1.741AsnMet: 1.741 ± 0.161
3.393AsnAsn: 3.393 ± 0.257
3.163AsnPro: 3.163 ± 0.217
2.189AsnGln: 2.189 ± 0.193
2.778AsnArg: 2.778 ± 0.178
3.034AsnSer: 3.034 ± 0.183
3.739AsnThr: 3.739 ± 0.209
3.611AsnVal: 3.611 ± 0.256
0.755AsnTrp: 0.755 ± 0.13
2.343AsnTyr: 2.343 ± 0.169
0.0AsnXaa: 0.0 ± 0.0
Pro
2.753ProAla: 2.753 ± 0.264
0.218ProCys: 0.218 ± 0.057
2.753ProAsp: 2.753 ± 0.21
3.406ProGlu: 3.406 ± 0.207
2.087ProPhe: 2.087 ± 0.162
2.561ProGly: 2.561 ± 0.206
0.627ProHis: 0.627 ± 0.087
2.919ProIle: 2.919 ± 0.19
2.702ProLys: 2.702 ± 0.185
2.663ProLeu: 2.663 ± 0.182
1.063ProMet: 1.063 ± 0.112
2.676ProAsn: 2.676 ± 0.188
1.101ProPro: 1.101 ± 0.112
1.28ProGln: 1.28 ± 0.144
1.383ProArg: 1.383 ± 0.136
2.484ProSer: 2.484 ± 0.171
2.906ProThr: 2.906 ± 0.211
3.431ProVal: 3.431 ± 0.277
0.499ProTrp: 0.499 ± 0.081
1.536ProTyr: 1.536 ± 0.158
0.0ProXaa: 0.0 ± 0.0
Gln
2.407GlnAla: 2.407 ± 0.211
0.333GlnCys: 0.333 ± 0.067
1.805GlnAsp: 1.805 ± 0.156
1.985GlnGlu: 1.985 ± 0.137
1.69GlnPhe: 1.69 ± 0.127
2.087GlnGly: 2.087 ± 0.179
0.717GlnHis: 0.717 ± 0.098
2.305GlnIle: 2.305 ± 0.172
1.511GlnLys: 1.511 ± 0.147
3.252GlnLeu: 3.252 ± 0.242
0.896GlnMet: 0.896 ± 0.099
1.434GlnAsn: 1.434 ± 0.176
1.524GlnPro: 1.524 ± 0.158
1.408GlnGln: 1.408 ± 0.128
1.844GlnArg: 1.844 ± 0.163
2.061GlnSer: 2.061 ± 0.16
2.049GlnThr: 2.049 ± 0.181
2.446GlnVal: 2.446 ± 0.145
0.435GlnTrp: 0.435 ± 0.066
1.485GlnTyr: 1.485 ± 0.135
0.0GlnXaa: 0.0 ± 0.0
Arg
2.714ArgAla: 2.714 ± 0.2
0.41ArgCys: 0.41 ± 0.074
2.791ArgAsp: 2.791 ± 0.18
2.753ArgGlu: 2.753 ± 0.195
2.01ArgPhe: 2.01 ± 0.197
2.919ArgGly: 2.919 ± 0.241
0.922ArgHis: 0.922 ± 0.108
3.175ArgIle: 3.175 ± 0.222
3.022ArgLys: 3.022 ± 0.227
3.931ArgLeu: 3.931 ± 0.254
1.511ArgMet: 1.511 ± 0.134
2.548ArgAsn: 2.548 ± 0.183
1.46ArgPro: 1.46 ± 0.166
1.626ArgGln: 1.626 ± 0.155
2.01ArgArg: 2.01 ± 0.165
2.702ArgSer: 2.702 ± 0.205
2.65ArgThr: 2.65 ± 0.212
2.983ArgVal: 2.983 ± 0.174
0.461ArgTrp: 0.461 ± 0.071
2.305ArgTyr: 2.305 ± 0.183
0.0ArgXaa: 0.0 ± 0.0
Ser
3.419SerAla: 3.419 ± 0.241
0.371SerCys: 0.371 ± 0.073
3.406SerAsp: 3.406 ± 0.241
3.508SerGlu: 3.508 ± 0.221
3.175SerPhe: 3.175 ± 0.208
4.148SerGly: 4.148 ± 0.263
1.088SerHis: 1.088 ± 0.13
4.353SerIle: 4.353 ± 0.202
4.2SerLys: 4.2 ± 0.243
4.853SerLeu: 4.853 ± 0.245
1.69SerMet: 1.69 ± 0.184
3.598SerAsn: 3.598 ± 0.188
2.292SerPro: 2.292 ± 0.218
1.69SerGln: 1.69 ± 0.132
2.842SerArg: 2.842 ± 0.199
3.752SerSer: 3.752 ± 0.253
3.521SerThr: 3.521 ± 0.21
4.443SerVal: 4.443 ± 0.247
0.743SerTrp: 0.743 ± 0.118
2.484SerTyr: 2.484 ± 0.193
0.0SerXaa: 0.0 ± 0.0
Thr
3.828ThrAla: 3.828 ± 0.238
0.32ThrCys: 0.32 ± 0.055
3.7ThrAsp: 3.7 ± 0.225
3.585ThrGlu: 3.585 ± 0.217
3.099ThrPhe: 3.099 ± 0.261
5.019ThrGly: 5.019 ± 0.324
1.165ThrHis: 1.165 ± 0.122
4.814ThrIle: 4.814 ± 0.265
3.995ThrLys: 3.995 ± 0.225
5.506ThrLeu: 5.506 ± 0.264
1.498ThrMet: 1.498 ± 0.136
3.547ThrAsn: 3.547 ± 0.223
3.137ThrPro: 3.137 ± 0.256
1.857ThrGln: 1.857 ± 0.159
2.714ThrArg: 2.714 ± 0.212
3.816ThrSer: 3.816 ± 0.232
4.392ThrThr: 4.392 ± 0.334
5.019ThrVal: 5.019 ± 0.323
0.781ThrTrp: 0.781 ± 0.099
2.638ThrTyr: 2.638 ± 0.209
0.0ThrXaa: 0.0 ± 0.0
Val
3.764ValAla: 3.764 ± 0.245
0.563ValCys: 0.563 ± 0.09
4.993ValAsp: 4.993 ± 0.262
5.07ValGlu: 5.07 ± 0.285
2.586ValPhe: 2.586 ± 0.18
4.2ValGly: 4.2 ± 0.251
1.076ValHis: 1.076 ± 0.127
5.25ValIle: 5.25 ± 0.292
5.186ValLys: 5.186 ± 0.266
4.814ValLeu: 4.814 ± 0.25
1.729ValMet: 1.729 ± 0.161
4.11ValAsn: 4.11 ± 0.272
2.983ValPro: 2.983 ± 0.273
2.394ValGln: 2.394 ± 0.196
3.227ValArg: 3.227 ± 0.179
4.52ValSer: 4.52 ± 0.236
4.853ValThr: 4.853 ± 0.33
4.494ValVal: 4.494 ± 0.296
0.807ValTrp: 0.807 ± 0.118
2.676ValTyr: 2.676 ± 0.205
0.0ValXaa: 0.0 ± 0.0
Trp
0.615TrpAla: 0.615 ± 0.092
0.179TrpCys: 0.179 ± 0.057
0.999TrpAsp: 0.999 ± 0.151
0.883TrpGlu: 0.883 ± 0.102
0.538TrpPhe: 0.538 ± 0.078
0.691TrpGly: 0.691 ± 0.109
0.192TrpHis: 0.192 ± 0.051
0.781TrpIle: 0.781 ± 0.097
0.794TrpLys: 0.794 ± 0.094
0.96TrpLeu: 0.96 ± 0.097
0.359TrpMet: 0.359 ± 0.078
0.832TrpAsn: 0.832 ± 0.164
0.256TrpPro: 0.256 ± 0.06
0.333TrpGln: 0.333 ± 0.058
0.589TrpArg: 0.589 ± 0.088
0.781TrpSer: 0.781 ± 0.108
0.743TrpThr: 0.743 ± 0.11
0.896TrpVal: 0.896 ± 0.11
0.166TrpTrp: 0.166 ± 0.041
0.589TrpTyr: 0.589 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.151TyrAla: 2.151 ± 0.179
0.448TyrCys: 0.448 ± 0.074
2.894TyrAsp: 2.894 ± 0.184
2.049TyrGlu: 2.049 ± 0.173
1.805TyrPhe: 1.805 ± 0.156
2.817TyrGly: 2.817 ± 0.189
0.947TyrHis: 0.947 ± 0.115
2.766TyrIle: 2.766 ± 0.166
2.484TyrLys: 2.484 ± 0.163
3.431TyrLeu: 3.431 ± 0.269
1.14TyrMet: 1.14 ± 0.125
2.522TyrAsn: 2.522 ± 0.171
1.869TyrPro: 1.869 ± 0.177
1.626TyrGln: 1.626 ± 0.142
2.151TyrArg: 2.151 ± 0.191
2.458TyrSer: 2.458 ± 0.172
2.663TyrThr: 2.663 ± 0.184
2.446TyrVal: 2.446 ± 0.169
0.627TyrTrp: 0.627 ± 0.099
1.805TyrTyr: 1.805 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 257 proteins (78103 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski