Psittacus erithacus timneh papillomavirus (PePV)
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8WJW4|F8WJW4_PEPV Putative E7 protein OS=Psittacus erithacus timneh papillomavirus OX=197772 GN=E7 PE=4 SV=1
MM1 pKa = 7.12 RR2 pKa = 11.84 TMRR5 pKa = 11.84 YY6 pKa = 9.1 HH7 pKa = 6.81 YY8 pKa = 8.2 PTEE11 pKa = 4.4 TDD13 pKa = 3.68 DD14 pKa = 3.97 SSPDD18 pKa = 3.2 EE19 pKa = 4.82 GEE21 pKa = 4.33 TGNHH25 pKa = 5.86 AVTHH29 pKa = 6.88 LLMQLQEE36 pKa = 4.11 QLHH39 pKa = 5.99 ALNYY43 pKa = 7.07 PTDD46 pKa = 4.42 DD47 pKa = 4.57 SDD49 pKa = 5.23 DD50 pKa = 3.85 STDD53 pKa = 3.53 GEE55 pKa = 4.25 EE56 pKa = 4.04 LVFRR60 pKa = 11.84 LTVEE64 pKa = 4.66 EE65 pKa = 4.54 SASEE69 pKa = 4.12 DD70 pKa = 4.03 DD71 pKa = 3.59 NDD73 pKa = 3.75 SRR75 pKa = 11.84 QSLSVDD81 pKa = 3.4 DD82 pKa = 4.74 VGAEE86 pKa = 3.65 VDD88 pKa = 3.97 VEE90 pKa = 4.38 VEE92 pKa = 3.93 GAYY95 pKa = 10.48 GGVASDD101 pKa = 4.37 NLLCHH106 pKa = 6.77 EE107 pKa = 4.85 SMDD110 pKa = 3.99 DD111 pKa = 3.7 PEE113 pKa = 4.48 YY114 pKa = 11.12 SGASVGSRR122 pKa = 11.84 PDD124 pKa = 3.55 GYY126 pKa = 10.68 DD127 pKa = 3.07 EE128 pKa = 4.24 RR129 pKa = 11.84 APWKK133 pKa = 9.29 CTICGRR139 pKa = 11.84 PVTPQEE145 pKa = 3.78 LATFGVVNPWNKK157 pKa = 10.04 QGVCTVCFHH166 pKa = 6.58 GQQEE170 pKa = 4.5 RR171 pKa = 11.84 FNSIWGG177 pKa = 3.6
Molecular weight: 19.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.815
IPC2_protein 4.037
IPC_protein 4.012
Toseland 3.821
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.897
Rodwell 3.846
Grimsley 3.732
Solomon 3.986
Lehninger 3.935
Nozaki 4.101
DTASelect 4.304
Thurlkill 3.859
EMBOSS 3.91
Sillero 4.139
Patrickios 1.926
IPC_peptide 3.986
IPC2_peptide 4.113
IPC2.peptide.svr19 4.008
Protein with the highest isoelectric point:
>tr|F8WJW3|F8WJW3_PEPV Major capsid protein L1 OS=Psittacus erithacus timneh papillomavirus OX=197772 GN=L1 PE=3 SV=1
MM1 pKa = 7.26 EE2 pKa = 4.65 TTNPTISAMCNCCTPSDD19 pKa = 3.89 HH20 pKa = 6.27 KK21 pKa = 9.45 TLRR24 pKa = 11.84 LYY26 pKa = 10.94 LSEE29 pKa = 4.11 WQDD32 pKa = 3.51 IIPVSDD38 pKa = 4.25 EE39 pKa = 3.79 EE40 pKa = 4.54 HH41 pKa = 6.49 GPPTVRR47 pKa = 11.84 AISFDD52 pKa = 3.13 ASTADD57 pKa = 3.48 MEE59 pKa = 4.75 TQRR62 pKa = 11.84 KK63 pKa = 7.46 LCKK66 pKa = 9.64 YY67 pKa = 10.1 CLIVLLKK74 pKa = 10.45 RR75 pKa = 11.84 LGSILRR81 pKa = 11.84 LGILIDD87 pKa = 4.28 PIKK90 pKa = 10.32 ILRR93 pKa = 11.84 RR94 pKa = 11.84 DD95 pKa = 3.48 AQGQGPRR102 pKa = 11.84 TPHH105 pKa = 5.95 GLLPRR110 pKa = 11.84 RR111 pKa = 11.84 CTFLLNLRR119 pKa = 11.84 IRR121 pKa = 11.84 LEE123 pKa = 4.36 EE124 pKa = 3.88 ILLPEE129 pKa = 4.81 LPLDD133 pKa = 3.81 RR134 pKa = 11.84 LILMVDD140 pKa = 3.85 LQDD143 pKa = 3.52 VSHH146 pKa = 7.45 LYY148 pKa = 10.45 RR149 pKa = 11.84 LLSPTVDD156 pKa = 3.05 EE157 pKa = 5.12 TYY159 pKa = 10.71 LQVGHH164 pKa = 7.3 IMEE167 pKa = 4.63 RR168 pKa = 11.84 MEE170 pKa = 4.08 HH171 pKa = 5.52 ACRR174 pKa = 11.84 RR175 pKa = 11.84 RR176 pKa = 11.84 MKK178 pKa = 10.07 RR179 pKa = 11.84 HH180 pKa = 4.83 QKK182 pKa = 10.02 RR183 pKa = 11.84 GRR185 pKa = 11.84 LKK187 pKa = 10.68 DD188 pKa = 3.52 RR189 pKa = 11.84 NYY191 pKa = 10.68 FNAAA195 pKa = 3.19
Molecular weight: 22.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.41
IPC2_protein 8.287
IPC_protein 8.244
Toseland 8.17
ProMoST 8.712
Dawson 8.916
Bjellqvist 9.194
Wikipedia 8.887
Rodwell 8.96
Grimsley 7.98
Solomon 9.092
Lehninger 9.092
Nozaki 9.428
DTASelect 8.931
Thurlkill 9.004
EMBOSS 9.107
Sillero 9.297
Patrickios 4.774
IPC_peptide 9.077
IPC2_peptide 8.39
IPC2.peptide.svr19 8.151
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2489
177
678
414.8
46.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.187 ± 0.736
1.687 ± 0.499
7.111 ± 0.477
5.303 ± 0.73
3.294 ± 0.331
7.714 ± 0.724
2.33 ± 0.639
4.821 ± 0.472
2.853 ± 0.836
8.357 ± 0.783
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.848 ± 0.353
3.294 ± 0.43
7.674 ± 0.926
3.495 ± 0.186
7.754 ± 0.634
7.312 ± 0.582
7.473 ± 0.59
6.468 ± 0.509
1.527 ± 0.255
3.495 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here