Parapedobacter indicus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Parapedobacter

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5134 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3KKB6|A0A1I3KKB6_9SPHI Thiol-disulfide isomerase or thioredoxin OS=Parapedobacter indicus OX=1477437 GN=SAMN05444682_105227 PE=4 SV=1
DDD2 pKa = 3.73HHH4 pKa = 6.59LTYYY8 pKa = 9.94DDD10 pKa = 3.92EE11 pKa = 5.14GDDD14 pKa = 3.75VNIDDD19 pKa = 3.36NVGRR23 pKa = 11.84LEEE26 pKa = 4.07YY27 pKa = 10.97DD28 pKa = 3.37DDD30 pKa = 3.67HHH32 pKa = 6.75LTYYY36 pKa = 9.94DDD38 pKa = 3.92EE39 pKa = 5.14GDDD42 pKa = 3.75VNIDDD47 pKa = 3.36NVGRR51 pKa = 11.84LEEE54 pKa = 4.07YY55 pKa = 10.97DD56 pKa = 3.37DDD58 pKa = 3.67HHH60 pKa = 6.75LTYYY64 pKa = 10.07DDD66 pKa = 3.82EE67 pKa = 4.73GDDD70 pKa = 3.94VDDD73 pKa = 3.18DDD75 pKa = 3.72NVGKKK80 pKa = 10.72EEE82 pKa = 4.06YY83 pKa = 10.84DD84 pKa = 3.63DDD86 pKa = 3.85HHH88 pKa = 6.75LTYYY92 pKa = 10.07DDD94 pKa = 3.82EE95 pKa = 4.73GDDD98 pKa = 3.94VDDD101 pKa = 3.18DDD103 pKa = 3.72NVGKKK108 pKa = 10.72EEE110 pKa = 4.06YY111 pKa = 10.84DD112 pKa = 3.63DDD114 pKa = 3.85HHH116 pKa = 6.75LTYYY120 pKa = 10.07DDD122 pKa = 3.82EE123 pKa = 4.73GDDD126 pKa = 3.94VDDD129 pKa = 3.18DDD131 pKa = 3.72NVGKKK136 pKa = 10.72EEE138 pKa = 4.06YY139 pKa = 10.84DD140 pKa = 3.63DDD142 pKa = 3.85HHH144 pKa = 6.75LTYYY148 pKa = 9.94DDD150 pKa = 3.92EE151 pKa = 5.14GDDD154 pKa = 3.75VNIDDD159 pKa = 3.36NVGRR163 pKa = 11.84LEEE166 pKa = 4.07YY167 pKa = 10.97DD168 pKa = 3.37DDD170 pKa = 3.67HHH172 pKa = 6.75LTYYY176 pKa = 10.07DDD178 pKa = 3.82EE179 pKa = 4.73GDDD182 pKa = 3.94VDDD185 pKa = 3.18DDD187 pKa = 3.72NVGKKK192 pKa = 10.72EEE194 pKa = 4.06YY195 pKa = 10.84DD196 pKa = 3.63DDD198 pKa = 3.85HHH200 pKa = 6.75LTYYY204 pKa = 9.94DDD206 pKa = 3.92EE207 pKa = 5.14GDDD210 pKa = 3.75VNIDDD215 pKa = 3.36NVGRR219 pKa = 11.84LEEE222 pKa = 4.07YY223 pKa = 10.97DD224 pKa = 3.37DDD226 pKa = 3.67HHH228 pKa = 6.75LTYYY232 pKa = 10.05DDD234 pKa = 4.33EE235 pKa = 4.74GQTTNLDDD243 pKa = 3.31APVVKKK249 pKa = 10.56LEEE252 pKa = 4.38VTEEE256 pKa = 4.43GSEEE260 pKa = 3.9TTNTDDD266 pKa = 1.86DDD268 pKa = 3.66VYYY271 pKa = 10.76LTYYY275 pKa = 10.0AEEE278 pKa = 4.24DD279 pKa = 3.91VDDD282 pKa = 3.42DD283 pKa = 5.87IDDD286 pKa = 3.73KKK288 pKa = 11.49NNGLNIDDD296 pKa = 3.96TSGAIQLGGPLIKKK310 pKa = 10.32KKK312 pKa = 8.4TIQTDDD318 pKa = 3.18DDD320 pKa = 3.65TLAIAGLEEE329 pKa = 4.12YY330 pKa = 10.39TATDDD335 pKa = 3.59DD336 pKa = 4.81KK337 pKa = 11.13VVADDD342 pKa = 5.49ITGEEE347 pKa = 4.23KKK349 pKa = 10.58LKKK352 pKa = 10.26AMPKKK357 pKa = 9.82FYYY360 pKa = 9.4PSIIIPTAADDD371 pKa = 3.3VEEE374 pKa = 4.47PSEEE378 pKa = 4.2EE379 pKa = 4.16FGTIDDD385 pKa = 5.34YYY387 pKa = 11.34KK388 pKa = 10.4KK389 pKa = 8.75YY390 pKa = 10.13KKK392 pKa = 10.32FGSPQVSSPGAQPLPVLPANEEE414 pKa = 4.16YYY416 pKa = 10.93YY417 pKa = 11.03YY418 pKa = 8.31TWYYY422 pKa = 10.54DD423 pKa = 3.18MVFEEE428 pKa = 5.55ITVDDD433 pKa = 3.0EE434 pKa = 4.56YY435 pKa = 11.72VLRR438 pKa = 11.84YYY440 pKa = 9.07EE441 pKa = 4.53KKK443 pKa = 10.41DDD445 pKa = 3.46DDD447 pKa = 3.55TLGSFMNIVFAVKKK461 pKa = 10.39NL

Molecular weight: 51.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3FF86|A0A1I3FF86_9SPHI Alpha-1 2-mannosidase putative OS=Parapedobacter indicus OX=1477437 GN=SAMN05444682_102300 PE=4 SV=1
MM1 pKa = 7.45NLTFNAEE8 pKa = 3.61QKK10 pKa = 9.78MRR12 pKa = 11.84VGPSDD17 pKa = 2.57RR18 pKa = 11.84VYY20 pKa = 10.58RR21 pKa = 11.84HH22 pKa = 6.46RR23 pKa = 11.84FQTTPMLLRR32 pKa = 11.84LKK34 pKa = 10.56AVVVSDD40 pKa = 3.01SGKK43 pKa = 8.73RR44 pKa = 11.84HH45 pKa = 4.75QRR47 pKa = 11.84RR48 pKa = 11.84CQVSVRR54 pKa = 11.84KK55 pKa = 10.16VNASEE60 pKa = 3.52PSMRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 10.33DD68 pKa = 3.38EE69 pKa = 4.03VMSEE73 pKa = 4.0PMSTNVIGNSMEE85 pKa = 4.4VTCLLSMRR93 pKa = 11.84HH94 pKa = 4.82PAYY97 pKa = 10.03RR98 pKa = 11.84RR99 pKa = 11.84HH100 pKa = 5.66EE101 pKa = 4.58LNTGFCLEE109 pKa = 4.19QEE111 pKa = 4.26NLSWRR116 pKa = 11.84CEE118 pKa = 3.75GKK120 pKa = 8.43TPSGGHH126 pKa = 6.01RR127 pKa = 11.84KK128 pKa = 10.22GEE130 pKa = 4.31STDD133 pKa = 3.47ALHH136 pKa = 7.35GGGTLRR142 pKa = 11.84SSDD145 pKa = 4.07EE146 pKa = 4.25VPVMRR151 pKa = 11.84MEE153 pKa = 3.74RR154 pKa = 11.84RR155 pKa = 11.84GRR157 pKa = 11.84IIRR160 pKa = 11.84PTSLSEE166 pKa = 3.95KK167 pKa = 8.41PFKK170 pKa = 10.78RR171 pKa = 11.84EE172 pKa = 3.49VSRR175 pKa = 11.84EE176 pKa = 3.61LGKK179 pKa = 10.64RR180 pKa = 11.84LEE182 pKa = 4.21QGQFRR187 pKa = 11.84TT188 pKa = 4.11

Molecular weight: 21.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5134

0

5134

1905442

24

3921

371.1

41.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.078 ± 0.04

0.757 ± 0.011

5.716 ± 0.025

5.881 ± 0.03

4.588 ± 0.028

7.468 ± 0.039

2.098 ± 0.018

6.406 ± 0.026

5.179 ± 0.037

9.536 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.014

4.866 ± 0.03

4.158 ± 0.021

3.83 ± 0.022

5.13 ± 0.029

6.075 ± 0.024

5.859 ± 0.043

6.728 ± 0.029

1.383 ± 0.015

4.073 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski