Gilliamella bombicola
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C4BFB1|A0A1C4BFB1_9GAMM PTS system mannose-specific IIA component OS=Gilliamella bombicola OX=1798182 GN=GA0061081_104130 PE=4 SV=1
MM1 pKa = 7.5 EE2 pKa = 6.02 FDD4 pKa = 3.48 TSILCDD10 pKa = 4.03 YY11 pKa = 10.26 YY12 pKa = 10.5 PEE14 pKa = 5.73 DD15 pKa = 3.83 INVVEE20 pKa = 5.24 PIFSNFGGVTSFSGQAVTVKK40 pKa = 10.42 CFEE43 pKa = 4.95 DD44 pKa = 3.41 NGLLYY49 pKa = 10.48 EE50 pKa = 4.69 ILQSDD55 pKa = 4.62 GSGKK59 pKa = 9.89 ILVIDD64 pKa = 3.93 GGGSVRR70 pKa = 11.84 RR71 pKa = 11.84 ALIDD75 pKa = 3.75 AEE77 pKa = 4.99 LIDD80 pKa = 5.52 IAIQNQWAGIIVYY93 pKa = 9.72 GAVRR97 pKa = 11.84 QVDD100 pKa = 4.06 YY101 pKa = 10.88 LAEE104 pKa = 4.79 ADD106 pKa = 3.97 IGIQALAAIPVGAEE120 pKa = 3.77 DD121 pKa = 3.71 QGVGEE126 pKa = 3.91 IDD128 pKa = 2.93 IRR130 pKa = 11.84 VNFGGVTFFSGDD142 pKa = 3.81 FIYY145 pKa = 10.94 ADD147 pKa = 3.31 NTGIILSEE155 pKa = 3.73 IALEE159 pKa = 4.46 IEE161 pKa = 4.67 DD162 pKa = 4.17 KK163 pKa = 11.28
Molecular weight: 17.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.643
IPC_protein 3.63
Toseland 3.414
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.567
Rodwell 3.465
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.745
DTASelect 3.948
Thurlkill 3.478
EMBOSS 3.567
Sillero 3.745
Patrickios 1.825
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.725
Protein with the highest isoelectric point:
>tr|A0A1C3ZG96|A0A1C3ZG96_9GAMM Elongation factor P OS=Gilliamella bombicola OX=1798182 GN=efp PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.44 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LTVASS46 pKa = 3.91
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2103
0
2103
664647
39
2011
316.0
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.44 ± 0.058
1.139 ± 0.018
5.387 ± 0.039
5.36 ± 0.055
4.31 ± 0.045
6.348 ± 0.053
2.081 ± 0.023
8.224 ± 0.052
6.386 ± 0.045
10.316 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.028
5.311 ± 0.042
3.753 ± 0.031
4.532 ± 0.043
3.998 ± 0.038
6.513 ± 0.056
5.459 ± 0.036
6.382 ± 0.046
1.109 ± 0.017
3.533 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here