Circovirus-like genome RW-D

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GII2|C6GII2_9VIRU ATP-dependent helicase Rep OS=Circovirus-like genome RW-D OX=642254 PE=3 SV=1
MM1 pKa = 7.56LAQAPGRR8 pKa = 11.84YY9 pKa = 8.38RR10 pKa = 11.84NWVITVNNWTEE21 pKa = 3.73NDD23 pKa = 3.41YY24 pKa = 11.5KK25 pKa = 10.99LALLSPYY32 pKa = 9.86RR33 pKa = 11.84YY34 pKa = 9.45IIIGRR39 pKa = 11.84EE40 pKa = 3.58RR41 pKa = 11.84GEE43 pKa = 4.45CNTPHH48 pKa = 5.99LQIYY52 pKa = 8.59LQLYY56 pKa = 7.88HH57 pKa = 7.12AKK59 pKa = 10.6SFDD62 pKa = 3.55TVKK65 pKa = 10.92QNFFPRR71 pKa = 11.84AHH73 pKa = 6.79LEE75 pKa = 4.06ASHH78 pKa = 6.33SKK80 pKa = 9.66PRR82 pKa = 11.84QARR85 pKa = 11.84AYY87 pKa = 7.14CTKK90 pKa = 10.01EE91 pKa = 3.54HH92 pKa = 5.85YY93 pKa = 10.25FEE95 pKa = 4.77YY96 pKa = 11.37GEE98 pKa = 4.65MSTQGKK104 pKa = 7.89RR105 pKa = 11.84TDD107 pKa = 3.47LQVAQEE113 pKa = 4.53LLDD116 pKa = 3.7SGISIRR122 pKa = 11.84QALEE126 pKa = 3.82SEE128 pKa = 5.22MITSMGALAAYY139 pKa = 7.87EE140 pKa = 4.29KK141 pKa = 10.11LQKK144 pKa = 10.51YY145 pKa = 6.5YY146 pKa = 9.75TVHH149 pKa = 6.6RR150 pKa = 11.84PRR152 pKa = 11.84PRR154 pKa = 11.84VVWIYY159 pKa = 11.22GPAGSGKK166 pKa = 7.72TDD168 pKa = 3.02KK169 pKa = 10.63AYY171 pKa = 10.73SISGPDD177 pKa = 3.36VYY179 pKa = 11.06KK180 pKa = 10.84SDD182 pKa = 4.78LIKK185 pKa = 10.85EE186 pKa = 4.36GWFDD190 pKa = 3.46GYY192 pKa = 11.11DD193 pKa = 3.08RR194 pKa = 11.84HH195 pKa = 7.13RR196 pKa = 11.84SIIIDD201 pKa = 3.78DD202 pKa = 4.28LEE204 pKa = 4.99IDD206 pKa = 4.06RR207 pKa = 11.84DD208 pKa = 3.97DD209 pKa = 4.06KK210 pKa = 11.31KK211 pKa = 9.54TFGLLLSLLDD221 pKa = 4.42KK222 pKa = 11.09NPQKK226 pKa = 11.4VNVKK230 pKa = 9.99GSSASILADD239 pKa = 3.61TIVITCQQAPWHH251 pKa = 6.01IWYY254 pKa = 9.3HH255 pKa = 5.99PSDD258 pKa = 3.89NMSLPFKK265 pKa = 10.91HH266 pKa = 5.7MATRR270 pKa = 11.84EE271 pKa = 3.98EE272 pKa = 4.06IEE274 pKa = 4.1RR275 pKa = 11.84DD276 pKa = 2.94VDD278 pKa = 3.28LRR280 pKa = 11.84QIMRR284 pKa = 11.84RR285 pKa = 11.84ITEE288 pKa = 4.69IIHH291 pKa = 6.23LTDD294 pKa = 3.29TDD296 pKa = 3.7KK297 pKa = 11.56VKK299 pKa = 10.77YY300 pKa = 10.23PEE302 pKa = 4.03VTEE305 pKa = 4.22VV306 pKa = 2.91

Molecular weight: 35.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GII2|C6GII2_9VIRU ATP-dependent helicase Rep OS=Circovirus-like genome RW-D OX=642254 PE=3 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84PAYY7 pKa = 9.96VGPSWGGSRR16 pKa = 11.84IGGANIPMNTRR27 pKa = 11.84AKK29 pKa = 9.85RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 9.6IIPLLWNSGSKK45 pKa = 9.9LANNLNLGFGSRR57 pKa = 11.84GRR59 pKa = 11.84GPGSMVSRR67 pKa = 11.84IRR69 pKa = 11.84NYY71 pKa = 10.82KK72 pKa = 9.19MEE74 pKa = 4.48ISVKK78 pKa = 9.99PVGTGSSYY86 pKa = 11.02SYY88 pKa = 10.68YY89 pKa = 10.45RR90 pKa = 11.84YY91 pKa = 8.07TRR93 pKa = 11.84RR94 pKa = 11.84PDD96 pKa = 2.87KK97 pKa = 10.71GSRR100 pKa = 11.84IVKK103 pKa = 7.41TQQAPLFSVFNSGSRR118 pKa = 11.84MTSLQGEE125 pKa = 4.25QGYY128 pKa = 6.47TTLSVMTGAKK138 pKa = 9.57LRR140 pKa = 11.84SLYY143 pKa = 11.04LEE145 pKa = 4.34TSANQDD151 pKa = 2.46GRR153 pKa = 11.84FWVGYY158 pKa = 9.54ARR160 pKa = 11.84CRR162 pKa = 11.84WMFQNQSEE170 pKa = 4.67ATTHH174 pKa = 4.75LTIYY178 pKa = 10.37EE179 pKa = 4.09FTTRR183 pKa = 11.84RR184 pKa = 11.84DD185 pKa = 3.78SNVGPSLAFQSGLASIQAGVGSNASDD211 pKa = 3.0IGATPFMTPRR221 pKa = 11.84FTEE224 pKa = 3.58NFRR227 pKa = 11.84ILKK230 pKa = 9.67KK231 pKa = 10.87YY232 pKa = 10.38SVEE235 pKa = 3.82LAQGRR240 pKa = 11.84SHH242 pKa = 6.89IHH244 pKa = 4.71TALYY248 pKa = 10.61RR249 pKa = 11.84MNKK252 pKa = 9.61NYY254 pKa = 10.36SDD256 pKa = 4.31SLYY259 pKa = 11.26QMDD262 pKa = 4.21GSSDD266 pKa = 3.62VVLGGWTRR274 pKa = 11.84GLFVIAHH281 pKa = 5.68GTPYY285 pKa = 11.04NSFATKK291 pKa = 10.01TNVSTTPVAIDD302 pKa = 3.12IVANEE307 pKa = 4.4TYY309 pKa = 8.61HH310 pKa = 7.01TYY312 pKa = 11.07TNLQNKK318 pKa = 10.02SIFEE322 pKa = 4.25VQSDD326 pKa = 4.43FPTFTDD332 pKa = 4.18GRR334 pKa = 11.84IIDD337 pKa = 4.27IGSGDD342 pKa = 3.97PEE344 pKa = 4.44AVDD347 pKa = 3.72EE348 pKa = 4.48VV349 pKa = 3.77

Molecular weight: 38.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

655

306

349

327.5

37.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.107 ± 0.071

0.611 ± 0.255

5.191 ± 1.155

4.58 ± 1.125

3.359 ± 0.74

7.481 ± 1.781

2.443 ± 0.796

6.565 ± 0.883

5.038 ± 1.035

7.023 ± 0.792

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.213

4.427 ± 1.027

4.427 ± 0.102

4.122 ± 0.313

7.786 ± 0.638

8.702 ± 1.496

7.176 ± 0.893

5.344 ± 0.305

1.679 ± 0.194

5.344 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski