Streptococcus phage Javan264

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AXD1|A0A4D6AXD1_9CAUD Phage_Mu_F domain-containing protein OS=Streptococcus phage Javan264 OX=2548081 GN=Javan264_0026 PE=4 SV=1
MM1 pKa = 6.75QHH3 pKa = 6.41GLFGTFDD10 pKa = 3.74YY11 pKa = 11.39DD12 pKa = 3.23NWLSTYY18 pKa = 9.63EE19 pKa = 3.82GHH21 pKa = 6.89EE22 pKa = 4.08EE23 pKa = 4.1VFQGDD28 pKa = 3.2EE29 pKa = 4.05DD30 pKa = 3.8EE31 pKa = 6.53AYY33 pKa = 10.53DD34 pKa = 4.23RR35 pKa = 11.84WKK37 pKa = 10.85DD38 pKa = 3.68DD39 pKa = 4.57QLEE42 pKa = 4.15DD43 pKa = 3.19WW44 pKa = 4.52

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AYA5|A0A4D6AYA5_9CAUD DUF3799 domain-containing protein OS=Streptococcus phage Javan264 OX=2548081 GN=Javan264_0018 PE=4 SV=1
MM1 pKa = 7.77GKK3 pKa = 8.66NQHH6 pKa = 5.58VVPAKK11 pKa = 10.06NGGWNVKK18 pKa = 9.83GAGNSRR24 pKa = 11.84ATAHH28 pKa = 5.61TTTKK32 pKa = 10.7SEE34 pKa = 3.87ATNIARR40 pKa = 11.84QISRR44 pKa = 11.84NQGSEE49 pKa = 4.28LIIHH53 pKa = 6.65GKK55 pKa = 9.66DD56 pKa = 3.08GKK58 pKa = 9.98IQSRR62 pKa = 11.84DD63 pKa = 3.33SHH65 pKa = 6.86GKK67 pKa = 10.26DD68 pKa = 3.06PFPPKK73 pKa = 10.63GG74 pKa = 3.28

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

8967

43

1349

203.8

22.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.13 ± 1.261

0.546 ± 0.146

6.201 ± 0.567

6.792 ± 0.751

4.037 ± 0.255

6.412 ± 0.516

1.327 ± 0.195

6.513 ± 0.325

8.085 ± 0.589

7.896 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.922 ± 0.32

5.688 ± 0.307

2.966 ± 0.158

4.572 ± 0.351

3.636 ± 0.438

6.557 ± 0.59

6.345 ± 0.277

6.401 ± 0.273

1.048 ± 0.138

3.926 ± 0.588
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski