Heliobacillus mobilis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae; Heliobacterium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3745 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I3SKG2|A0A6I3SKG2_HELMO Chemotaxis protein CheA OS=Heliobacillus mobilis OX=28064 GN=GJ688_10590 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 5.83KK3 pKa = 10.35DD4 pKa = 3.42TWTHH8 pKa = 7.06LINVADD14 pKa = 4.01VYY16 pKa = 10.26EE17 pKa = 4.71AEE19 pKa = 4.35VVEE22 pKa = 5.85SILQEE27 pKa = 3.9ANIPFLRR34 pKa = 11.84KK35 pKa = 9.43YY36 pKa = 10.74VGTDD40 pKa = 3.25GYY42 pKa = 11.34LKK44 pKa = 10.18IVTGGTFNNVEE55 pKa = 4.34LYY57 pKa = 10.62VPEE60 pKa = 4.4IFYY63 pKa = 11.11DD64 pKa = 3.67DD65 pKa = 3.94AVNLISISYY74 pKa = 10.31EE75 pKa = 3.69EE76 pKa = 5.28DD77 pKa = 3.13NSSDD81 pKa = 3.07EE82 pKa = 4.84TYY84 pKa = 10.8QQDD87 pKa = 3.3KK88 pKa = 10.09SHH90 pKa = 6.46WSEE93 pKa = 4.22IILIAIAFSLLYY105 pKa = 10.8GLFMVLANQQ114 pKa = 3.71

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I3SQC5|A0A6I3SQC5_HELMO DUF2238 domain-containing protein OS=Heliobacillus mobilis OX=28064 GN=GJ688_18405 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.47QPKK8 pKa = 8.64NRR10 pKa = 11.84KK11 pKa = 8.1HH12 pKa = 5.86KK13 pKa = 9.85RR14 pKa = 11.84VHH16 pKa = 5.97GFLSRR21 pKa = 11.84MSTPNGRR28 pKa = 11.84NVLKK32 pKa = 10.53RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3745

0

3745

1150309

29

2138

307.2

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.281 ± 0.043

1.042 ± 0.016

5.175 ± 0.028

6.946 ± 0.045

3.786 ± 0.028

7.434 ± 0.042

1.972 ± 0.019

6.627 ± 0.036

5.474 ± 0.035

9.926 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.018

3.659 ± 0.025

4.41 ± 0.036

4.09 ± 0.03

5.662 ± 0.042

5.925 ± 0.04

5.484 ± 0.034

7.38 ± 0.037

1.12 ± 0.014

2.967 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski