Mycobacterium phage JF4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T1T0|A0A6M3T1T0_9CAUD Uncharacterized protein OS=Mycobacterium phage JF4 OX=2725641 GN=73 PE=4 SV=1
MM1 pKa = 7.61TLPKK5 pKa = 10.51NLTSHH10 pKa = 6.41SDD12 pKa = 3.43EE13 pKa = 4.39VDD15 pKa = 2.53WDD17 pKa = 3.8AVHH20 pKa = 7.46DD21 pKa = 4.09YY22 pKa = 10.92VYY24 pKa = 10.98EE25 pKa = 5.18GDD27 pKa = 4.83DD28 pKa = 4.06DD29 pKa = 4.96NEE31 pKa = 4.05

Molecular weight: 3.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T3N5|A0A6M3T3N5_9CAUD Helix-turn-helix DNA binding domain protein OS=Mycobacterium phage JF4 OX=2725641 GN=46 PE=4 SV=1
MM1 pKa = 7.25MIVARR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84VALGVASAGTVAVGGLAFALSFTALRR34 pKa = 11.84DD35 pKa = 3.56LSASNGVAQAWMVPLVVDD53 pKa = 3.79GGIIVATAATVALRR67 pKa = 11.84RR68 pKa = 11.84HH69 pKa = 5.22QWYY72 pKa = 9.42AWTLLILSSLVSVAGNVAHH91 pKa = 6.57AQAHH95 pKa = 5.49GAIAMVIAAIPPLWLLAATHH115 pKa = 5.73LTVMLSRR122 pKa = 11.84SDD124 pKa = 3.85KK125 pKa = 10.87EE126 pKa = 4.24PVPVAAEE133 pKa = 3.9PLHH136 pKa = 6.32IANAAA141 pKa = 3.29

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14331

27

1014

181.4

20.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.664 ± 0.379

0.809 ± 0.113

6.315 ± 0.267

6.887 ± 0.307

3.308 ± 0.216

8.185 ± 0.434

2.128 ± 0.2

4.668 ± 0.166

4.284 ± 0.226

8.213 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.456 ± 0.154

3.538 ± 0.211

5.792 ± 0.341

3.817 ± 0.33

6.866 ± 0.358

5.185 ± 0.217

5.652 ± 0.222

7.424 ± 0.241

1.947 ± 0.128

2.861 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski