Solibacter usitatus (strain Ellin6076)

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Bryobacterales; Solibacteraceae; Candidatus Solibacter; Candidatus Solibacter usitatus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q01T94|Q01T94_SOLUE Uncharacterized protein OS=Solibacter usitatus (strain Ellin6076) OX=234267 GN=Acid_6200 PE=4 SV=1
MM1 pKa = 7.22SVVDD5 pKa = 4.29RR6 pKa = 11.84EE7 pKa = 4.27FSEE10 pKa = 4.43EE11 pKa = 4.0LTGLMPAVAHH21 pKa = 6.41RR22 pKa = 11.84LGGGACAGYY31 pKa = 9.92IIAVVEE37 pKa = 4.57SNTVEE42 pKa = 4.48LRR44 pKa = 11.84CNLCGEE50 pKa = 4.36IVGVVQVGIMEE61 pKa = 4.62GLLGLDD67 pKa = 4.12CDD69 pKa = 4.77EE70 pKa = 5.07EE71 pKa = 4.46PSPQDD76 pKa = 3.39EE77 pKa = 4.81PEE79 pKa = 4.06DD80 pKa = 3.6

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q020U4|Q020U4_SOLUE Tetratricopeptide TPR_2 repeat protein OS=Solibacter usitatus (strain Ellin6076) OX=234267 GN=Acid_3573 PE=4 SV=1
MM1 pKa = 7.35SAKK4 pKa = 10.18SCQLCGKK11 pKa = 8.43PLSRR15 pKa = 11.84LRR17 pKa = 11.84VGGDD21 pKa = 3.38GEE23 pKa = 4.5FCSRR27 pKa = 11.84EE28 pKa = 3.72HH29 pKa = 6.67RR30 pKa = 11.84NQYY33 pKa = 8.89GLRR36 pKa = 11.84RR37 pKa = 11.84GMDD40 pKa = 3.14RR41 pKa = 11.84LQEE44 pKa = 4.0VDD46 pKa = 3.94KK47 pKa = 10.56VTSLMRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 4.18SPRR59 pKa = 11.84QISPARR65 pKa = 11.84LMCNTALATRR75 pKa = 11.84GFLQPRR81 pKa = 11.84TYY83 pKa = 11.06GAGAEE88 pKa = 4.28LAPFAPIFRR97 pKa = 11.84IPEE100 pKa = 3.89PPRR103 pKa = 11.84LSKK106 pKa = 10.58VAEE109 pKa = 4.15RR110 pKa = 11.84YY111 pKa = 7.74VAPRR115 pKa = 11.84TARR118 pKa = 11.84FEE120 pKa = 5.26GIMQVRR126 pKa = 11.84RR127 pKa = 11.84SDD129 pKa = 3.33TRR131 pKa = 11.84QLRR134 pKa = 11.84ITGRR138 pKa = 11.84NSALEE143 pKa = 3.87MPAPKK148 pKa = 9.81RR149 pKa = 11.84RR150 pKa = 11.84LTTQVPQAPMADD162 pKa = 3.76LHH164 pKa = 6.11CTVPAAAASRR174 pKa = 11.84RR175 pKa = 11.84NFALLRR181 pKa = 11.84QTEE184 pKa = 4.25IRR186 pKa = 11.84VHH188 pKa = 7.87AGMPPASLRR197 pKa = 11.84QIALPGTSALGRR209 pKa = 11.84NLPNRR214 pKa = 11.84KK215 pKa = 9.16VGATPLEE222 pKa = 4.58GNALRR227 pKa = 11.84VSIALAFGAPGVSRR241 pKa = 11.84RR242 pKa = 11.84VFVTPPAMSSALVWPRR258 pKa = 11.84TAHH261 pKa = 5.82GVWPEE266 pKa = 4.19TKK268 pKa = 9.3NTTARR273 pKa = 11.84PRR275 pKa = 11.84NLAVRR280 pKa = 11.84MTNPQTCLPAARR292 pKa = 11.84EE293 pKa = 4.2GQRR296 pKa = 11.84RR297 pKa = 11.84SQFVFPGPLRR307 pKa = 11.84PGKK310 pKa = 9.93QKK312 pKa = 10.46PRR314 pKa = 11.84TNGMPPARR322 pKa = 11.84TTDD325 pKa = 3.51VSWILSDD332 pKa = 3.74PRR334 pKa = 11.84TGGIGVPPTSAGFAKK349 pKa = 10.57RR350 pKa = 11.84NGVHH354 pKa = 6.86LCNLTLAPTTTNPTHH369 pKa = 6.75LVAFRR374 pKa = 11.84PFLPQEE380 pKa = 4.04PVGCPMVPFEE390 pKa = 4.48GTTAASIITAPAAGAPGTAADD411 pKa = 4.43APPAAEE417 pKa = 3.84EE418 pKa = 4.07AAPVAQVRR426 pKa = 11.84LEE428 pKa = 3.9EE429 pKa = 4.38HH430 pKa = 6.65FGSGWEE436 pKa = 3.93NWEE439 pKa = 4.11GGTNDD444 pKa = 3.41WLVDD448 pKa = 3.62VAGVRR453 pKa = 11.84TGSLALFQPTMDD465 pKa = 3.63LVDD468 pKa = 3.88YY469 pKa = 10.22EE470 pKa = 5.03LEE472 pKa = 3.81FLARR476 pKa = 11.84IDD478 pKa = 3.82TRR480 pKa = 11.84SLTWVVRR487 pKa = 11.84ATDD490 pKa = 3.41LQEE493 pKa = 4.08YY494 pKa = 7.73MRR496 pKa = 11.84CTLTAIPGGEE506 pKa = 4.24LEE508 pKa = 4.17FSRR511 pKa = 11.84TVVRR515 pKa = 11.84SGIGEE520 pKa = 4.08EE521 pKa = 4.23TVIAPVRR528 pKa = 11.84IPGKK532 pKa = 9.05PRR534 pKa = 11.84AAMTVRR540 pKa = 11.84TRR542 pKa = 11.84VSGDD546 pKa = 3.33TFSVTVDD553 pKa = 3.47GKK555 pKa = 10.86DD556 pKa = 3.01MDD558 pKa = 3.56TWQEE562 pKa = 3.69DD563 pKa = 3.22RR564 pKa = 11.84LYY566 pKa = 10.89CGGIGFMGAHH576 pKa = 7.82DD577 pKa = 4.42DD578 pKa = 3.63RR579 pKa = 11.84ARR581 pKa = 11.84LYY583 pKa = 8.91WVRR586 pKa = 11.84ISSSDD591 pKa = 3.77NIGKK595 pKa = 8.33EE596 pKa = 4.12HH597 pKa = 6.09QKK599 pKa = 10.78KK600 pKa = 10.05

Molecular weight: 65.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7761

0

7761

2980018

37

4666

384.0

41.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.392 ± 0.033

0.899 ± 0.008

4.985 ± 0.023

5.217 ± 0.035

3.995 ± 0.018

8.555 ± 0.029

2.0 ± 0.013

4.791 ± 0.017

3.451 ± 0.026

9.813 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.012

3.35 ± 0.028

5.591 ± 0.022

3.562 ± 0.016

6.834 ± 0.032

5.977 ± 0.031

5.796 ± 0.041

7.324 ± 0.023

1.474 ± 0.012

2.766 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski