Coxiella burnetii (strain RSA 493 / Nine Mile phase I)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Coxiella; Coxiella burnetii

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1812 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q83DY2|Q83DY2_COXBU LPS-assembly lipoprotein LptE OS=Coxiella burnetii (strain RSA 493 / Nine Mile phase I) OX=227377 GN=lptE PE=3 SV=1
MM1 pKa = 7.02TFVVIDD7 pKa = 3.17NCIRR11 pKa = 11.84CKK13 pKa = 10.25YY14 pKa = 7.51TDD16 pKa = 3.93CVEE19 pKa = 4.15VCPVDD24 pKa = 4.11CFRR27 pKa = 11.84EE28 pKa = 4.92GPNMLVIDD36 pKa = 4.61PDD38 pKa = 3.57EE39 pKa = 5.96CIDD42 pKa = 4.49CNLCVPEE49 pKa = 4.98CPVDD53 pKa = 4.65AIFAEE58 pKa = 4.79DD59 pKa = 4.23DD60 pKa = 3.81LPEE63 pKa = 4.28EE64 pKa = 3.92KK65 pKa = 10.36HH66 pKa = 6.82AFLEE70 pKa = 4.49MNADD74 pKa = 3.82LAKK77 pKa = 10.1RR78 pKa = 11.84WPLITAKK85 pKa = 10.6KK86 pKa = 8.86EE87 pKa = 3.99APPDD91 pKa = 3.88ADD93 pKa = 4.18DD94 pKa = 3.52WTEE97 pKa = 4.12VPDD100 pKa = 3.56KK101 pKa = 10.93LQYY104 pKa = 10.88LEE106 pKa = 4.89EE107 pKa = 4.3EE108 pKa = 4.3WPKK111 pKa = 11.4

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P53591|SUCD_COXBU Succinate--CoA ligase [ADP-forming] subunit alpha OS=Coxiella burnetii (strain RSA 493 / Nine Mile phase I) OX=227377 GN=sucD PE=3 SV=2
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 8.9QKK11 pKa = 10.63RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 6.85GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.43GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1812

0

1812

512564

11

1626

282.9

31.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.059 ± 0.063

1.121 ± 0.023

4.544 ± 0.041

6.055 ± 0.07

4.546 ± 0.049

6.353 ± 0.058

2.414 ± 0.025

7.38 ± 0.053

6.272 ± 0.062

10.992 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.024

4.197 ± 0.043

4.422 ± 0.032

4.171 ± 0.039

5.347 ± 0.042

5.939 ± 0.043

5.125 ± 0.032

6.244 ± 0.046

1.202 ± 0.026

3.308 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski