Lactobacillus virus ATCC8014
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4HZK9|K4HZK9_9CAUD Uncharacterized protein OS=Lactobacillus virus ATCC8014 OX=1225794 GN=8014-B1_0044 PE=4 SV=1
MM1 pKa = 7.28 TLYY4 pKa = 10.88 NVGDD8 pKa = 3.86 TVRR11 pKa = 11.84 LYY13 pKa = 10.88 DD14 pKa = 3.49 GTVGVVDD21 pKa = 3.87 VVTSGGALLIRR32 pKa = 11.84 GQYY35 pKa = 7.71 VQPDD39 pKa = 3.65 DD40 pKa = 3.75 VFNVCRR46 pKa = 11.84 KK47 pKa = 9.49 LKK49 pKa = 10.4 PGDD52 pKa = 3.51 VVTFKK57 pKa = 10.59 PVYY60 pKa = 9.36 EE61 pKa = 4.32 LRR63 pKa = 11.84 QCPLWGQYY71 pKa = 11.1 GDD73 pKa = 5.26 DD74 pKa = 4.8 DD75 pKa = 5.89 LDD77 pKa = 3.8 TVFGIRR83 pKa = 11.84 TADD86 pKa = 3.22 IPKK89 pKa = 9.41 KK90 pKa = 9.64 ATVEE94 pKa = 4.13 VCHH97 pKa = 6.86 CYY99 pKa = 10.03 PGSDD103 pKa = 3.44 DD104 pKa = 3.71 VFTIEE109 pKa = 4.15 DD110 pKa = 3.76 SEE112 pKa = 4.84 YY113 pKa = 10.93 SFLSSMVEE121 pKa = 4.09 RR122 pKa = 11.84 IDD124 pKa = 3.51
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.082
IPC2_protein 4.228
IPC_protein 4.19
Toseland 3.973
ProMoST 4.279
Dawson 4.19
Bjellqvist 4.38
Wikipedia 4.139
Rodwell 4.012
Grimsley 3.884
Solomon 4.177
Lehninger 4.139
Nozaki 4.304
DTASelect 4.584
Thurlkill 4.024
EMBOSS 4.151
Sillero 4.317
Patrickios 2.905
IPC_peptide 4.177
IPC2_peptide 4.291
IPC2.peptide.svr19 4.233
Protein with the highest isoelectric point:
>tr|K4I1U5|K4I1U5_9CAUD Uncharacterized protein OS=Lactobacillus virus ATCC8014 OX=1225794 GN=8014-B1_0055 PE=4 SV=1
MM1 pKa = 6.51 TTIISSIFTFIIMALILLRR20 pKa = 11.84 IIALVAKK27 pKa = 9.46 PLIKK31 pKa = 10.16 PVAGTVGIIAVIVFIKK47 pKa = 10.56 KK48 pKa = 9.97 RR49 pKa = 11.84 FFSKK53 pKa = 10.31 KK54 pKa = 8.32 GAKK57 pKa = 9.09
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 10.54
IPC_protein 11.857
Toseland 12.12
ProMoST 12.515
Dawson 12.135
Bjellqvist 12.047
Wikipedia 12.544
Rodwell 12.398
Grimsley 12.164
Solomon 12.559
Lehninger 12.471
Nozaki 12.12
DTASelect 12.047
Thurlkill 12.12
EMBOSS 12.603
Sillero 12.12
Patrickios 12.149
IPC_peptide 12.559
IPC2_peptide 11.491
IPC2.peptide.svr19 8.822
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
11682
33
1084
194.7
21.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.236 ± 0.623
0.608 ± 0.142
7.071 ± 0.347
5.239 ± 0.411
3.724 ± 0.173
7.516 ± 0.443
1.721 ± 0.149
6.121 ± 0.302
6.531 ± 0.277
7.105 ± 0.241
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.244 ± 0.248
5.419 ± 0.243
3.116 ± 0.196
3.116 ± 0.162
4.845 ± 0.389
6.026 ± 0.357
6.335 ± 0.232
7.55 ± 0.308
1.121 ± 0.132
4.357 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here