Lactococcus lactis phage P475

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus P475

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9R1P4|R9R1P4_9CAUD Uncharacterized protein OS=Lactococcus lactis phage P475 OX=213774 GN=P475_0015 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 5.5FDD4 pKa = 6.13SYY6 pKa = 11.42IDD8 pKa = 3.32WYY10 pKa = 11.58NNLLTMPLNDD20 pKa = 3.86VILGVKK26 pKa = 8.96DD27 pKa = 3.57TIEE30 pKa = 4.55DD31 pKa = 3.52KK32 pKa = 10.66TVYY35 pKa = 10.62LSLSDD40 pKa = 3.95SKK42 pKa = 10.99VLKK45 pKa = 9.75MDD47 pKa = 3.09NTSFVMGYY55 pKa = 9.13YY56 pKa = 8.74YY57 pKa = 10.45QVVLSVKK64 pKa = 10.52DD65 pKa = 3.51VDD67 pKa = 4.41DD68 pKa = 4.44EE69 pKa = 4.52LVGLVGDD76 pKa = 4.15VLQNGWNMTNWSEE89 pKa = 4.32NSHH92 pKa = 6.58LYY94 pKa = 10.7NYY96 pKa = 8.61TGTVYY101 pKa = 10.45LPCGSGGQAWQQ112 pKa = 3.18

Molecular weight: 12.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9R1N8|R9R1N8_9CAUD Putative prohead protease OS=Lactococcus lactis phage P475 OX=213774 GN=P475_005 PE=4 SV=1
MM1 pKa = 7.78IILLFIIMLFISPRR15 pKa = 11.84IALLILLLAINPVFVLLWLLVWLAIKK41 pKa = 10.62LKK43 pKa = 10.74

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

9262

32

916

162.5

18.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.143 ± 0.626

0.583 ± 0.141

5.841 ± 0.222

7.677 ± 0.56

4.276 ± 0.327

6.295 ± 0.616

1.296 ± 0.188

6.845 ± 0.273

9.577 ± 0.524

8.422 ± 0.404

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.133

6.672 ± 0.259

2.224 ± 0.256

3.649 ± 0.239

3.552 ± 0.284

6.187 ± 0.441

6.111 ± 0.28

6.305 ± 0.398

1.555 ± 0.147

4.297 ± 0.464
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski