Sinorhizobium phage phiLM21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076G6X5|A0A076G6X5_9CAUD Uncharacterized protein OS=Sinorhizobium phage phiLM21 OX=1524882 GN=phiLM21_p018 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84TYY5 pKa = 10.48YY6 pKa = 10.81KK7 pKa = 10.61SFEE10 pKa = 4.18RR11 pKa = 11.84DD12 pKa = 2.68NGQEE16 pKa = 3.85VTVEE20 pKa = 3.82YY21 pKa = 10.59SITPYY26 pKa = 11.05DD27 pKa = 3.69PGVSYY32 pKa = 11.17GPAEE36 pKa = 3.87SCYY39 pKa = 9.12PPEE42 pKa = 5.44GGEE45 pKa = 4.0VEE47 pKa = 4.22IVKK50 pKa = 9.83VFSDD54 pKa = 4.86DD55 pKa = 4.39APDD58 pKa = 4.14LAWTDD63 pKa = 3.85KK64 pKa = 11.4EE65 pKa = 4.6DD66 pKa = 5.27DD67 pKa = 3.76DD68 pKa = 3.87WCARR72 pKa = 11.84IAEE75 pKa = 4.47THH77 pKa = 6.33DD78 pKa = 3.3HH79 pKa = 6.93SDD81 pKa = 3.57YY82 pKa = 11.71ADD84 pKa = 4.52DD85 pKa = 4.45PDD87 pKa = 3.51YY88 pKa = 11.2WRR90 pKa = 11.84DD91 pKa = 3.28EE92 pKa = 4.09

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076GCY8|A0A076GCY8_9CAUD NinB family protein OS=Sinorhizobium phage phiLM21 OX=1524882 GN=phiLM21_p007 PE=4 SV=1
MM1 pKa = 6.95TAPMLLDD8 pKa = 3.59SEE10 pKa = 4.59QAAEE14 pKa = 4.28LLNVSTRR21 pKa = 11.84TLRR24 pKa = 11.84EE25 pKa = 3.52FVKK28 pKa = 10.84AGDD31 pKa = 3.36IAYY34 pKa = 9.92VPLGAGRR41 pKa = 11.84SKK43 pKa = 10.69PRR45 pKa = 11.84LGFTMDD51 pKa = 5.83DD52 pKa = 2.95INEE55 pKa = 4.6FIKK58 pKa = 10.61SRR60 pKa = 11.84RR61 pKa = 11.84TRR63 pKa = 11.84EE64 pKa = 4.11CPSTSQRR71 pKa = 11.84TARR74 pKa = 11.84ITTSTSKK81 pKa = 10.84SVVLGFTALQKK92 pKa = 10.55QRR94 pKa = 11.84TAEE97 pKa = 3.94KK98 pKa = 10.07QKK100 pKa = 10.24QGKK103 pKa = 8.62RR104 pKa = 3.42

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

15212

55

940

211.3

23.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.622 ± 0.376

0.986 ± 0.149

6.048 ± 0.244

6.515 ± 0.346

3.392 ± 0.139

8.086 ± 0.455

1.834 ± 0.181

5.174 ± 0.19

4.891 ± 0.312

7.356 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.162

3.524 ± 0.239

4.47 ± 0.268

3.681 ± 0.199

7.027 ± 0.386

5.962 ± 0.334

5.995 ± 0.323

6.797 ± 0.27

1.735 ± 0.147

2.577 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski