Heterobasidion irregulare (strain TC 32-1)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Russulales; Bondarzewiaceae; Heterobasidion; Heterobasidion annosum species complex; Heterobasidion irregulare

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4KID9|W4KID9_HETIT Phosphoglycerate kinase OS=Heterobasidion irregulare (strain TC 32-1) OX=747525 GN=HETIRDRAFT_407577 PE=3 SV=1
MM1 pKa = 7.26FPSPSWLDD9 pKa = 3.84DD10 pKa = 4.19DD11 pKa = 5.96DD12 pKa = 7.29ASTLDD17 pKa = 4.01NNASTLDD24 pKa = 3.96DD25 pKa = 4.01NASLLDD31 pKa = 3.99NNDD34 pKa = 3.61AGHH37 pKa = 6.69FSHH40 pKa = 7.2SGTCSLAPSASDD52 pKa = 5.41LDD54 pKa = 4.45LDD56 pKa = 4.01PCGLLGTPEE65 pKa = 4.17PTLSLSDD72 pKa = 3.98DD73 pKa = 3.88EE74 pKa = 5.15EE75 pKa = 5.33PIPEE79 pKa = 4.61LKK81 pKa = 10.79NNPQYY86 pKa = 9.51QTSEE90 pKa = 3.92EE91 pKa = 4.1LFEE94 pKa = 4.88CFLDD98 pKa = 4.11SGSEE102 pKa = 3.95HH103 pKa = 7.55LDD105 pKa = 3.69DD106 pKa = 5.54NNSNPPAFDD115 pKa = 4.34KK116 pKa = 11.34DD117 pKa = 3.58PAICNAYY124 pKa = 7.44ITAYY128 pKa = 10.9LLTACHH134 pKa = 6.9RR135 pKa = 11.84STQEE139 pKa = 3.86AIKK142 pKa = 10.37AYY144 pKa = 10.62LDD146 pKa = 4.06AEE148 pKa = 4.32HH149 pKa = 6.97EE150 pKa = 4.5MLLFMQRR157 pKa = 11.84RR158 pKa = 11.84TGLEE162 pKa = 3.93MPGLDD167 pKa = 3.2TMAWTLLTLEE177 pKa = 4.44

Molecular weight: 19.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4JPK7|W4JPK7_HETIT Uncharacterized protein OS=Heterobasidion irregulare (strain TC 32-1) OX=747525 GN=HETIRDRAFT_442718 PE=4 SV=1
MM1 pKa = 7.53GSSCTARR8 pKa = 11.84CPPRR12 pKa = 11.84CPPRR16 pKa = 11.84RR17 pKa = 11.84LDD19 pKa = 3.36RR20 pKa = 11.84PSRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84VRR28 pKa = 11.84EE29 pKa = 3.56RR30 pKa = 11.84RR31 pKa = 11.84HH32 pKa = 5.61RR33 pKa = 11.84APLPRR38 pKa = 11.84SRR40 pKa = 11.84PSRR43 pKa = 11.84RR44 pKa = 11.84PPPRR48 pKa = 11.84AHH50 pKa = 6.54SPNLPRR56 pKa = 11.84PSPRR60 pKa = 11.84PRR62 pKa = 11.84TRR64 pKa = 11.84SSPRR68 pKa = 11.84PRR70 pKa = 11.84APRR73 pKa = 11.84APRR76 pKa = 11.84THH78 pKa = 6.42TRR80 pKa = 11.84PAIPTPSPRR89 pKa = 11.84TRR91 pKa = 11.84TRR93 pKa = 11.84THH95 pKa = 5.72TRR97 pKa = 11.84THH99 pKa = 4.95TRR101 pKa = 11.84RR102 pKa = 11.84SRR104 pKa = 11.84ARR106 pKa = 11.84RR107 pKa = 11.84SASRR111 pKa = 11.84GRR113 pKa = 11.84SRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84GMGRR121 pKa = 11.84PTGRR125 pKa = 11.84GTGRR129 pKa = 11.84ATTHH133 pKa = 6.11RR134 pKa = 11.84AIMPRR139 pKa = 11.84RR140 pKa = 11.84ALARR144 pKa = 11.84RR145 pKa = 11.84ALTRR149 pKa = 11.84RR150 pKa = 11.84GTARR154 pKa = 11.84RR155 pKa = 11.84GTARR159 pKa = 11.84RR160 pKa = 11.84GTARR164 pKa = 11.84RR165 pKa = 11.84GIMGRR170 pKa = 11.84DD171 pKa = 2.9TGRR174 pKa = 11.84RR175 pKa = 11.84IRR177 pKa = 11.84SRR179 pKa = 11.84RR180 pKa = 11.84CCRR183 pKa = 11.84RR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84RR188 pKa = 11.84GG189 pKa = 3.04

Molecular weight: 22.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13148

11

13159

5008163

30

5124

380.6

41.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.193 ± 0.025

1.271 ± 0.01

5.55 ± 0.017

5.61 ± 0.021

3.654 ± 0.014

6.602 ± 0.021

2.738 ± 0.012

4.712 ± 0.02

4.129 ± 0.019

9.247 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.079 ± 0.008

3.16 ± 0.014

6.744 ± 0.035

3.614 ± 0.016

6.766 ± 0.024

8.666 ± 0.028

5.907 ± 0.015

6.37 ± 0.017

1.424 ± 0.008

2.564 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski