Dipodomys ordii (Ord s kangaroo rat)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26567 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S3GI92|A0A1S3GI92_DIPOR Protein-arginine deiminase OS=Dipodomys ordii OX=10020 GN=Padi1 PE=3 SV=1
MM1 pKa = 7.58 TEE3 pKa = 3.84 TSVTEE8 pKa = 3.96 TSVTEE13 pKa = 4.1 TSVTDD18 pKa = 3.59 PSMTEE23 pKa = 3.77 TSVTGQCDD31 pKa = 3.29 RR32 pKa = 11.84 DD33 pKa = 3.5 QCDD36 pKa = 3.32 RR37 pKa = 11.84 DD38 pKa = 3.47 QCDD41 pKa = 3.29 RR42 pKa = 11.84 SHH44 pKa = 5.81 VTDD47 pKa = 4.68 PSVTDD52 pKa = 3.65 PSVTEE57 pKa = 4.03 TSVTDD62 pKa = 3.68 PSVTEE67 pKa = 3.89 TSVTEE72 pKa = 4.0 TSVTEE77 pKa = 4.0 TSVTEE82 pKa = 4.0 TSVTEE87 pKa = 4.1 TSVTDD92 pKa = 3.68 PSVTEE97 pKa = 4.03 TSVTDD102 pKa = 3.68 PSVTEE107 pKa = 3.89 TSVTEE112 pKa = 4.0 TSVTEE117 pKa = 4.1 TSVTDD122 pKa = 3.85 PSVTDD127 pKa = 3.98 PSVTDD132 pKa = 3.98 PSVTDD137 pKa = 3.98 PSVTDD142 pKa = 3.72 PSVTDD147 pKa = 3.16 QCDD150 pKa = 3.18 RR151 pKa = 11.84 PQCDD155 pKa = 2.55 RR156 pKa = 11.84 GQCHH160 pKa = 6.84 RR161 pKa = 11.84 GQCHH165 pKa = 6.57 RR166 pKa = 11.84 GQCHH170 pKa = 6.27 RR171 pKa = 11.84 PQCDD175 pKa = 3.4 GDD177 pKa = 3.75 PAAPTFIDD185 pKa = 3.76 TLDD188 pKa = 3.72 CALAA192 pKa = 4.47
Molecular weight: 20.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.516
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.859
Patrickios 1.1
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A1S3FZU8|A0A1S3FZU8_DIPOR stimulated by retinoic acid gene 6 protein homolog OS=Dipodomys ordii OX=10020 GN=LOC105993383 PE=4 SV=1
MM1 pKa = 7.11 SQNPQRR7 pKa = 11.84 RR8 pKa = 11.84 APSPLPWKK16 pKa = 8.91 RR17 pKa = 11.84 TSIKK21 pKa = 9.75 PPRR24 pKa = 11.84 PSTHH28 pKa = 5.92 TPRR31 pKa = 11.84 TPHH34 pKa = 6.38 PAPRR38 pKa = 11.84 TPHH41 pKa = 7.09 PDD43 pKa = 3.06 SAPRR47 pKa = 11.84 PLPHH51 pKa = 7.17 PSASRR56 pKa = 11.84 CRR58 pKa = 11.84 SGRR61 pKa = 11.84 PGRR64 pKa = 11.84 ARR66 pKa = 11.84 TRR68 pKa = 11.84 QALRR72 pKa = 11.84 RR73 pKa = 11.84 PPAAPQFLVEE83 pKa = 4.11 ATVRR87 pKa = 11.84 GPAKK91 pKa = 10.06 PPLPRR96 pKa = 11.84 TPAVAHH102 pKa = 6.0 AHH104 pKa = 5.81 RR105 pKa = 11.84 PGHH108 pKa = 5.96 RR109 pKa = 11.84 MTRR112 pKa = 11.84 TTPGHH117 pKa = 5.05 TRR119 pKa = 11.84 RR120 pKa = 11.84 PGPGRR125 pKa = 11.84 CRR127 pKa = 11.84 ASSQTLTRR135 pKa = 11.84 RR136 pKa = 11.84 SRR138 pKa = 11.84 LPWPPPRR145 pKa = 11.84 PHH147 pKa = 7.11 ARR149 pKa = 11.84 RR150 pKa = 11.84 LLPRR154 pKa = 11.84 GGPGRR159 pKa = 11.84 CSFPARR165 pKa = 11.84 PPSGARR171 pKa = 11.84 RR172 pKa = 11.84 AKK174 pKa = 9.67 PRR176 pKa = 11.84 SGVSRR181 pKa = 11.84 RR182 pKa = 11.84 RR183 pKa = 11.84 RR184 pKa = 11.84 RR185 pKa = 11.84 HH186 pKa = 4.35 RR187 pKa = 11.84 RR188 pKa = 11.84 RR189 pKa = 11.84 RR190 pKa = 11.84 RR191 pKa = 11.84 PLHH194 pKa = 5.45 FTSLPPPSMRR204 pKa = 11.84 PSHH207 pKa = 6.85 RR208 pKa = 11.84 GPPRR212 pKa = 11.84 ISWIRR217 pKa = 11.84 SRR219 pKa = 4.43
Molecular weight: 24.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.495
IPC2_protein 11.096
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.413
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.12
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.166
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19728
6839
26567
15473226
31
34334
582.4
64.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.921 ± 0.017
2.198 ± 0.013
4.802 ± 0.011
7.033 ± 0.021
3.675 ± 0.012
6.415 ± 0.019
2.608 ± 0.007
4.377 ± 0.015
5.681 ± 0.019
10.036 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.207 ± 0.006
3.615 ± 0.011
6.339 ± 0.025
4.817 ± 0.016
5.621 ± 0.014
8.436 ± 0.022
5.354 ± 0.014
6.023 ± 0.014
1.185 ± 0.005
2.65 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here