Asian prunus virus 1
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7B9H6|C7B9H6_9VIRU Movement protein TGB2 OS=Asian prunus virus 1 OX=351427 PE=2 SV=1
MM1 pKa = 7.37 EE2 pKa = 4.81 FVYY5 pKa = 10.83 DD6 pKa = 3.76 KK7 pKa = 10.84 LIEE10 pKa = 4.38 AGYY13 pKa = 10.22 IRR15 pKa = 11.84 TILQIDD21 pKa = 4.59 FPLIVHH27 pKa = 6.93 CIAGAGKK34 pKa = 8.38 STLIRR39 pKa = 11.84 EE40 pKa = 4.54 IIEE43 pKa = 3.87 SDD45 pKa = 3.39 SRR47 pKa = 11.84 FEE49 pKa = 3.99 AFTYY53 pKa = 9.41 GVPDD57 pKa = 3.92 PVNLSGIRR65 pKa = 11.84 IRR67 pKa = 11.84 GAEE70 pKa = 4.07 EE71 pKa = 3.44 LANARR76 pKa = 11.84 PEE78 pKa = 4.0 SFKK81 pKa = 10.96 IIDD84 pKa = 3.86 EE85 pKa = 4.66 YY86 pKa = 10.78 IGQHH90 pKa = 5.42 RR91 pKa = 11.84 PEE93 pKa = 4.14 GTAVCFADD101 pKa = 4.19 PNQFPYY107 pKa = 10.69 SSPNAHH113 pKa = 5.56 FTCYY117 pKa = 8.69 QSKK120 pKa = 10.29 RR121 pKa = 11.84 FGDD124 pKa = 3.54 QTCAFLGKK132 pKa = 9.66 LDD134 pKa = 4.3 CAAFSYY140 pKa = 10.81 KK141 pKa = 9.92 SDD143 pKa = 3.43 QIIFEE148 pKa = 4.62 TVFEE152 pKa = 4.61 GSIEE156 pKa = 4.04 DD157 pKa = 4.54 QIVCYY162 pKa = 9.86 EE163 pKa = 4.22 KK164 pKa = 10.88 EE165 pKa = 4.32 VFDD168 pKa = 5.13 LLDD171 pKa = 3.29 RR172 pKa = 11.84 HH173 pKa = 5.89 GADD176 pKa = 3.54 YY177 pKa = 11.13 KK178 pKa = 10.81 KK179 pKa = 10.47 DD180 pKa = 3.56 CQIRR184 pKa = 11.84 GSTFDD189 pKa = 2.92 IVTFITSSEE198 pKa = 4.13 SFEE201 pKa = 4.16 PEE203 pKa = 3.45 DD204 pKa = 3.59 RR205 pKa = 11.84 YY206 pKa = 10.98 KK207 pKa = 11.12 VYY209 pKa = 11.09 LCLTRR214 pKa = 11.84 HH215 pKa = 5.9 RR216 pKa = 11.84 SVLRR220 pKa = 11.84 ILSPEE225 pKa = 3.91 GMFLRR230 pKa = 11.84 DD231 pKa = 3.3 NAKK234 pKa = 10.19 FDD236 pKa = 3.6 ATSS239 pKa = 2.84
Molecular weight: 27.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.819
IPC2_protein 4.927
IPC_protein 4.851
Toseland 4.749
ProMoST 4.978
Dawson 4.825
Bjellqvist 4.965
Wikipedia 4.698
Rodwell 4.724
Grimsley 4.66
Solomon 4.825
Lehninger 4.774
Nozaki 4.94
DTASelect 5.092
Thurlkill 4.749
EMBOSS 4.724
Sillero 5.003
Patrickios 3.63
IPC_peptide 4.825
IPC2_peptide 4.991
IPC2.peptide.svr19 4.972
Protein with the highest isoelectric point:
>tr|C7B9H7|C7B9H7_9VIRU Movement protein TGBp3 OS=Asian prunus virus 1 OX=351427 PE=2 SV=1
MM1 pKa = 7.86 PSLTPPPDD9 pKa = 3.3 NTRR12 pKa = 11.84 VLLPIAVGLGVGIVIWCLTRR32 pKa = 11.84 STLPSVGDD40 pKa = 3.73 NVHH43 pKa = 6.34 SLPHH47 pKa = 6.23 GGNYY51 pKa = 10.08 LDD53 pKa = 3.48 GTKK56 pKa = 10.09 RR57 pKa = 11.84 ISYY60 pKa = 9.2 CGPRR64 pKa = 11.84 DD65 pKa = 3.73 SFPSSNLFKK74 pKa = 11.01 GGTFSAICIVVLLVFAIHH92 pKa = 6.53 VSEE95 pKa = 4.86 LFNRR99 pKa = 11.84 PNRR102 pKa = 11.84 RR103 pKa = 11.84 TCGCGSAAHH112 pKa = 6.43 AA113 pKa = 4.68
Molecular weight: 11.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.237
IPC2_protein 8.024
IPC_protein 8.039
Toseland 7.556
ProMoST 8.448
Dawson 8.565
Bjellqvist 9.033
Wikipedia 8.478
Rodwell 8.565
Grimsley 7.468
Solomon 8.77
Lehninger 8.799
Nozaki 9.355
DTASelect 8.682
Thurlkill 8.726
EMBOSS 8.785
Sillero 9.107
Patrickios 4.202
IPC_peptide 8.77
IPC2_peptide 8.799
IPC2.peptide.svr19 8.8
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2840
65
2022
568.0
63.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.479 ± 1.473
2.218 ± 0.508
4.648 ± 0.594
6.761 ± 0.851
6.162 ± 0.991
6.021 ± 0.789
2.465 ± 0.409
5.458 ± 0.856
5.88 ± 0.651
9.472 ± 1.301
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.972 ± 0.251
4.789 ± 0.759
4.366 ± 0.884
2.746 ± 0.578
5.634 ± 0.407
9.894 ± 0.896
5.035 ± 0.647
6.162 ± 1.218
0.915 ± 0.167
2.923 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here