Asian prunus virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C7B9H6|C7B9H6_9VIRU Movement protein TGB2 OS=Asian prunus virus 1 OX=351427 PE=2 SV=1
MM1 pKa = 7.37EE2 pKa = 4.81FVYY5 pKa = 10.83DD6 pKa = 3.76KK7 pKa = 10.84LIEE10 pKa = 4.38AGYY13 pKa = 10.22IRR15 pKa = 11.84TILQIDD21 pKa = 4.59FPLIVHH27 pKa = 6.93CIAGAGKK34 pKa = 8.38STLIRR39 pKa = 11.84EE40 pKa = 4.54IIEE43 pKa = 3.87SDD45 pKa = 3.39SRR47 pKa = 11.84FEE49 pKa = 3.99AFTYY53 pKa = 9.41GVPDD57 pKa = 3.92PVNLSGIRR65 pKa = 11.84IRR67 pKa = 11.84GAEE70 pKa = 4.07EE71 pKa = 3.44LANARR76 pKa = 11.84PEE78 pKa = 4.0SFKK81 pKa = 10.96IIDD84 pKa = 3.86EE85 pKa = 4.66YY86 pKa = 10.78IGQHH90 pKa = 5.42RR91 pKa = 11.84PEE93 pKa = 4.14GTAVCFADD101 pKa = 4.19PNQFPYY107 pKa = 10.69SSPNAHH113 pKa = 5.56FTCYY117 pKa = 8.69QSKK120 pKa = 10.29RR121 pKa = 11.84FGDD124 pKa = 3.54QTCAFLGKK132 pKa = 9.66LDD134 pKa = 4.3CAAFSYY140 pKa = 10.81KK141 pKa = 9.92SDD143 pKa = 3.43QIIFEE148 pKa = 4.62TVFEE152 pKa = 4.61GSIEE156 pKa = 4.04DD157 pKa = 4.54QIVCYY162 pKa = 9.86EE163 pKa = 4.22KK164 pKa = 10.88EE165 pKa = 4.32VFDD168 pKa = 5.13LLDD171 pKa = 3.29RR172 pKa = 11.84HH173 pKa = 5.89GADD176 pKa = 3.54YY177 pKa = 11.13KK178 pKa = 10.81KK179 pKa = 10.47DD180 pKa = 3.56CQIRR184 pKa = 11.84GSTFDD189 pKa = 2.92IVTFITSSEE198 pKa = 4.13SFEE201 pKa = 4.16PEE203 pKa = 3.45DD204 pKa = 3.59RR205 pKa = 11.84YY206 pKa = 10.98KK207 pKa = 11.12VYY209 pKa = 11.09LCLTRR214 pKa = 11.84HH215 pKa = 5.9RR216 pKa = 11.84SVLRR220 pKa = 11.84ILSPEE225 pKa = 3.91GMFLRR230 pKa = 11.84DD231 pKa = 3.3NAKK234 pKa = 10.19FDD236 pKa = 3.6ATSS239 pKa = 2.84

Molecular weight:
27.22 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C7B9H7|C7B9H7_9VIRU Movement protein TGBp3 OS=Asian prunus virus 1 OX=351427 PE=2 SV=1
MM1 pKa = 7.86PSLTPPPDD9 pKa = 3.3NTRR12 pKa = 11.84VLLPIAVGLGVGIVIWCLTRR32 pKa = 11.84STLPSVGDD40 pKa = 3.73NVHH43 pKa = 6.34SLPHH47 pKa = 6.23GGNYY51 pKa = 10.08LDD53 pKa = 3.48GTKK56 pKa = 10.09RR57 pKa = 11.84ISYY60 pKa = 9.2CGPRR64 pKa = 11.84DD65 pKa = 3.73SFPSSNLFKK74 pKa = 11.01GGTFSAICIVVLLVFAIHH92 pKa = 6.53VSEE95 pKa = 4.86LFNRR99 pKa = 11.84PNRR102 pKa = 11.84RR103 pKa = 11.84TCGCGSAAHH112 pKa = 6.43AA113 pKa = 4.68

Molecular weight:
11.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2840

65

2022

568.0

63.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.479 ± 1.473

2.218 ± 0.508

4.648 ± 0.594

6.761 ± 0.851

6.162 ± 0.991

6.021 ± 0.789

2.465 ± 0.409

5.458 ± 0.856

5.88 ± 0.651

9.472 ± 1.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.972 ± 0.251

4.789 ± 0.759

4.366 ± 0.884

2.746 ± 0.578

5.634 ± 0.407

9.894 ± 0.896

5.035 ± 0.647

6.162 ± 1.218

0.915 ± 0.167

2.923 ± 0.45

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski