Amino acid dipepetide frequency for Mycobacterium phage Bartholomew

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.299AlaAla: 17.299 ± 1.657
1.002AlaCys: 1.002 ± 0.265
8.883AlaAsp: 8.883 ± 0.963
10.486AlaGlu: 10.486 ± 1.38
3.072AlaPhe: 3.072 ± 0.489
9.084AlaGly: 9.084 ± 1.099
2.004AlaHis: 2.004 ± 0.403
6.011AlaIle: 6.011 ± 0.724
4.609AlaLys: 4.609 ± 0.62
10.019AlaLeu: 10.019 ± 0.772
2.672AlaMet: 2.672 ± 0.407
3.473AlaAsn: 3.473 ± 0.49
7.013AlaPro: 7.013 ± 0.707
4.275AlaGln: 4.275 ± 0.709
8.015AlaArg: 8.015 ± 0.784
6.479AlaSer: 6.479 ± 0.688
6.278AlaThr: 6.278 ± 0.747
6.946AlaVal: 6.946 ± 0.665
2.338AlaTrp: 2.338 ± 0.443
3.006AlaTyr: 3.006 ± 0.454
0.0AlaXaa: 0.0 ± 0.0
Cys
0.935CysAla: 0.935 ± 0.267
0.067CysCys: 0.067 ± 0.071
0.534CysAsp: 0.534 ± 0.186
0.868CysGlu: 0.868 ± 0.254
0.067CysPhe: 0.067 ± 0.064
1.269CysGly: 1.269 ± 0.299
0.468CysHis: 0.468 ± 0.151
0.2CysIle: 0.2 ± 0.119
0.2CysLys: 0.2 ± 0.11
0.668CysLeu: 0.668 ± 0.185
0.267CysMet: 0.267 ± 0.125
0.134CysAsn: 0.134 ± 0.093
1.002CysPro: 1.002 ± 0.345
0.401CysGln: 0.401 ± 0.152
0.801CysArg: 0.801 ± 0.219
0.401CysSer: 0.401 ± 0.183
0.935CysThr: 0.935 ± 0.256
0.2CysVal: 0.2 ± 0.118
0.2CysTrp: 0.2 ± 0.104
0.134CysTyr: 0.134 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
8.082AspAla: 8.082 ± 0.868
0.601AspCys: 0.601 ± 0.208
6.212AspAsp: 6.212 ± 0.647
5.811AspGlu: 5.811 ± 0.754
1.603AspPhe: 1.603 ± 0.327
6.612AspGly: 6.612 ± 0.88
1.269AspHis: 1.269 ± 0.266
2.204AspIle: 2.204 ± 0.285
1.67AspLys: 1.67 ± 0.271
5.61AspLeu: 5.61 ± 0.577
1.336AspMet: 1.336 ± 0.223
1.469AspAsn: 1.469 ± 0.257
4.542AspPro: 4.542 ± 0.588
1.469AspGln: 1.469 ± 0.35
5.143AspArg: 5.143 ± 0.525
2.672AspSer: 2.672 ± 0.602
3.206AspThr: 3.206 ± 0.474
4.007AspVal: 4.007 ± 0.49
1.803AspTrp: 1.803 ± 0.293
1.803AspTyr: 1.803 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
7.948GluAla: 7.948 ± 0.878
0.868GluCys: 0.868 ± 0.222
3.072GluAsp: 3.072 ± 0.478
2.738GluGlu: 2.738 ± 0.425
1.87GluPhe: 1.87 ± 0.345
4.074GluGly: 4.074 ± 0.57
1.87GluHis: 1.87 ± 0.418
3.74GluIle: 3.74 ± 0.565
1.469GluLys: 1.469 ± 0.358
6.612GluLeu: 6.612 ± 0.691
1.536GluMet: 1.536 ± 0.336
1.536GluAsn: 1.536 ± 0.295
3.874GluPro: 3.874 ± 0.587
2.538GluGln: 2.538 ± 0.363
3.941GluArg: 3.941 ± 0.506
3.139GluSer: 3.139 ± 0.599
3.072GluThr: 3.072 ± 0.442
3.807GluVal: 3.807 ± 0.494
1.536GluTrp: 1.536 ± 0.295
1.937GluTyr: 1.937 ± 0.355
0.0GluXaa: 0.0 ± 0.0
Phe
2.471PheAla: 2.471 ± 0.46
0.2PheCys: 0.2 ± 0.111
2.204PheAsp: 2.204 ± 0.405
1.937PheGlu: 1.937 ± 0.355
0.534PhePhe: 0.534 ± 0.16
3.807PheGly: 3.807 ± 0.543
0.534PheHis: 0.534 ± 0.181
1.536PheIle: 1.536 ± 0.333
1.069PheLys: 1.069 ± 0.22
1.603PheLeu: 1.603 ± 0.295
0.668PheMet: 0.668 ± 0.194
1.336PheAsn: 1.336 ± 0.335
1.67PhePro: 1.67 ± 0.39
1.069PheGln: 1.069 ± 0.268
1.67PheArg: 1.67 ± 0.313
1.202PheSer: 1.202 ± 0.349
1.469PheThr: 1.469 ± 0.327
2.137PheVal: 2.137 ± 0.309
0.334PheTrp: 0.334 ± 0.139
0.735PheTyr: 0.735 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
9.618GlyAla: 9.618 ± 0.963
0.401GlyCys: 0.401 ± 0.156
5.477GlyAsp: 5.477 ± 0.639
4.876GlyGlu: 4.876 ± 0.67
3.139GlyPhe: 3.139 ± 0.517
9.618GlyGly: 9.618 ± 1.323
1.336GlyHis: 1.336 ± 0.329
3.54GlyIle: 3.54 ± 0.482
3.006GlyLys: 3.006 ± 0.391
7.815GlyLeu: 7.815 ± 0.816
1.469GlyMet: 1.469 ± 0.334
2.338GlyAsn: 2.338 ± 0.343
4.408GlyPro: 4.408 ± 0.839
3.874GlyGln: 3.874 ± 0.722
5.944GlyArg: 5.944 ± 0.747
6.212GlySer: 6.212 ± 0.83
4.943GlyThr: 4.943 ± 0.65
7.213GlyVal: 7.213 ± 0.663
1.603GlyTrp: 1.603 ± 0.415
2.672GlyTyr: 2.672 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
1.536HisAla: 1.536 ± 0.288
0.134HisCys: 0.134 ± 0.094
1.87HisAsp: 1.87 ± 0.272
1.536HisGlu: 1.536 ± 0.389
0.267HisPhe: 0.267 ± 0.137
1.069HisGly: 1.069 ± 0.242
0.534HisHis: 0.534 ± 0.223
1.536HisIle: 1.536 ± 0.29
0.735HisLys: 0.735 ± 0.248
1.469HisLeu: 1.469 ± 0.302
0.334HisMet: 0.334 ± 0.152
0.668HisAsn: 0.668 ± 0.176
1.069HisPro: 1.069 ± 0.263
0.801HisGln: 0.801 ± 0.21
1.536HisArg: 1.536 ± 0.361
0.868HisSer: 0.868 ± 0.241
1.269HisThr: 1.269 ± 0.294
1.469HisVal: 1.469 ± 0.314
0.401HisTrp: 0.401 ± 0.179
0.601HisTyr: 0.601 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
6.011IleAla: 6.011 ± 0.555
0.334IleCys: 0.334 ± 0.144
3.072IleAsp: 3.072 ± 0.425
4.141IleGlu: 4.141 ± 0.613
0.468IlePhe: 0.468 ± 0.172
3.74IleGly: 3.74 ± 0.505
0.868IleHis: 0.868 ± 0.205
0.668IleIle: 0.668 ± 0.228
1.202IleLys: 1.202 ± 0.268
1.67IleLeu: 1.67 ± 0.279
0.534IleMet: 0.534 ± 0.163
1.336IleAsn: 1.336 ± 0.28
3.006IlePro: 3.006 ± 0.413
1.536IleGln: 1.536 ± 0.313
3.807IleArg: 3.807 ± 0.552
2.204IleSer: 2.204 ± 0.348
3.607IleThr: 3.607 ± 0.524
3.006IleVal: 3.006 ± 0.482
0.601IleTrp: 0.601 ± 0.173
1.002IleTyr: 1.002 ± 0.307
0.0IleXaa: 0.0 ± 0.0
Lys
4.141LysAla: 4.141 ± 0.487
0.134LysCys: 0.134 ± 0.119
1.269LysAsp: 1.269 ± 0.29
1.202LysGlu: 1.202 ± 0.257
1.336LysPhe: 1.336 ± 0.258
2.137LysGly: 2.137 ± 0.448
0.468LysHis: 0.468 ± 0.191
0.735LysIle: 0.735 ± 0.244
0.868LysLys: 0.868 ± 0.21
3.54LysLeu: 3.54 ± 0.455
0.935LysMet: 0.935 ± 0.233
0.868LysAsn: 0.868 ± 0.309
2.471LysPro: 2.471 ± 0.351
0.868LysGln: 0.868 ± 0.293
2.137LysArg: 2.137 ± 0.429
1.536LysSer: 1.536 ± 0.335
2.471LysThr: 2.471 ± 0.415
2.404LysVal: 2.404 ± 0.326
0.134LysTrp: 0.134 ± 0.099
0.735LysTyr: 0.735 ± 0.196
0.0LysXaa: 0.0 ± 0.0
Leu
10.553LeuAla: 10.553 ± 0.855
1.069LeuCys: 1.069 ± 0.227
6.145LeuAsp: 6.145 ± 0.646
3.607LeuGlu: 3.607 ± 0.494
1.469LeuPhe: 1.469 ± 0.249
7.948LeuGly: 7.948 ± 0.789
1.135LeuHis: 1.135 ± 0.28
4.408LeuIle: 4.408 ± 0.534
2.271LeuLys: 2.271 ± 0.299
6.679LeuLeu: 6.679 ± 0.729
1.469LeuMet: 1.469 ± 0.33
1.87LeuAsn: 1.87 ± 0.434
5.343LeuPro: 5.343 ± 0.532
2.338LeuGln: 2.338 ± 0.442
6.612LeuArg: 6.612 ± 0.595
4.408LeuSer: 4.408 ± 0.643
5.343LeuThr: 5.343 ± 0.656
4.275LeuVal: 4.275 ± 0.528
1.002LeuTrp: 1.002 ± 0.264
1.202LeuTyr: 1.202 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
4.007MetAla: 4.007 ± 0.517
0.2MetCys: 0.2 ± 0.114
0.601MetAsp: 0.601 ± 0.191
0.601MetGlu: 0.601 ± 0.225
0.668MetPhe: 0.668 ± 0.183
0.868MetGly: 0.868 ± 0.223
0.267MetHis: 0.267 ± 0.126
0.735MetIle: 0.735 ± 0.276
0.468MetLys: 0.468 ± 0.155
1.469MetLeu: 1.469 ± 0.346
0.334MetMet: 0.334 ± 0.139
0.801MetAsn: 0.801 ± 0.232
1.403MetPro: 1.403 ± 0.325
0.534MetGln: 0.534 ± 0.23
1.87MetArg: 1.87 ± 0.355
2.004MetSer: 2.004 ± 0.32
2.004MetThr: 2.004 ± 0.358
0.801MetVal: 0.801 ± 0.194
0.2MetTrp: 0.2 ± 0.116
0.401MetTyr: 0.401 ± 0.17
0.0MetXaa: 0.0 ± 0.0
Asn
4.007AsnAla: 4.007 ± 0.702
0.267AsnCys: 0.267 ± 0.14
1.403AsnAsp: 1.403 ± 0.364
1.603AsnGlu: 1.603 ± 0.314
0.401AsnPhe: 0.401 ± 0.16
3.674AsnGly: 3.674 ± 0.5
0.935AsnHis: 0.935 ± 0.247
0.935AsnIle: 0.935 ± 0.251
0.401AsnLys: 0.401 ± 0.156
1.87AsnLeu: 1.87 ± 0.312
0.668AsnMet: 0.668 ± 0.192
1.002AsnAsn: 1.002 ± 0.279
2.805AsnPro: 2.805 ± 0.353
0.334AsnGln: 0.334 ± 0.183
2.672AsnArg: 2.672 ± 0.524
2.338AsnSer: 2.338 ± 0.4
2.004AsnThr: 2.004 ± 0.435
1.403AsnVal: 1.403 ± 0.322
0.868AsnTrp: 0.868 ± 0.22
0.334AsnTyr: 0.334 ± 0.179
0.0AsnXaa: 0.0 ± 0.0
Pro
7.013ProAla: 7.013 ± 0.906
0.334ProCys: 0.334 ± 0.164
4.609ProAsp: 4.609 ± 0.472
4.007ProGlu: 4.007 ± 0.59
1.937ProPhe: 1.937 ± 0.318
6.746ProGly: 6.746 ± 0.724
1.069ProHis: 1.069 ± 0.279
1.737ProIle: 1.737 ± 0.311
1.937ProLys: 1.937 ± 0.347
4.876ProLeu: 4.876 ± 0.564
0.668ProMet: 0.668 ± 0.218
2.605ProAsn: 2.605 ± 0.542
3.139ProPro: 3.139 ± 0.567
2.605ProGln: 2.605 ± 0.439
4.675ProArg: 4.675 ± 0.67
2.538ProSer: 2.538 ± 0.4
3.674ProThr: 3.674 ± 0.488
5.21ProVal: 5.21 ± 0.578
1.269ProTrp: 1.269 ± 0.33
1.469ProTyr: 1.469 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
4.341GlnAla: 4.341 ± 0.654
0.267GlnCys: 0.267 ± 0.127
1.536GlnAsp: 1.536 ± 0.332
1.536GlnGlu: 1.536 ± 0.326
1.937GlnPhe: 1.937 ± 0.483
3.072GlnGly: 3.072 ± 0.721
0.601GlnHis: 0.601 ± 0.193
1.937GlnIle: 1.937 ± 0.372
1.403GlnLys: 1.403 ± 0.29
2.338GlnLeu: 2.338 ± 0.431
1.202GlnMet: 1.202 ± 0.235
0.735GlnAsn: 0.735 ± 0.25
2.204GlnPro: 2.204 ± 0.348
2.872GlnGln: 2.872 ± 0.501
2.538GlnArg: 2.538 ± 0.415
1.937GlnSer: 1.937 ± 0.383
1.937GlnThr: 1.937 ± 0.313
2.605GlnVal: 2.605 ± 0.49
0.935GlnTrp: 0.935 ± 0.219
0.468GlnTyr: 0.468 ± 0.16
0.0GlnXaa: 0.0 ± 0.0
Arg
8.816ArgAla: 8.816 ± 0.829
0.868ArgCys: 0.868 ± 0.236
5.41ArgAsp: 5.41 ± 0.525
3.941ArgGlu: 3.941 ± 0.634
2.404ArgPhe: 2.404 ± 0.362
5.277ArgGly: 5.277 ± 0.597
1.87ArgHis: 1.87 ± 0.371
3.139ArgIle: 3.139 ± 0.415
3.072ArgLys: 3.072 ± 0.452
4.809ArgLeu: 4.809 ± 0.504
2.071ArgMet: 2.071 ± 0.309
2.271ArgAsn: 2.271 ± 0.465
3.607ArgPro: 3.607 ± 0.477
2.538ArgGln: 2.538 ± 0.473
6.746ArgArg: 6.746 ± 1.021
3.406ArgSer: 3.406 ± 0.722
4.341ArgThr: 4.341 ± 0.584
4.809ArgVal: 4.809 ± 0.645
1.603ArgTrp: 1.603 ± 0.357
1.87ArgTyr: 1.87 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
7.013SerAla: 7.013 ± 0.624
0.534SerCys: 0.534 ± 0.19
3.273SerAsp: 3.273 ± 0.48
2.338SerGlu: 2.338 ± 0.489
1.336SerPhe: 1.336 ± 0.354
6.612SerGly: 6.612 ± 0.973
0.935SerHis: 0.935 ± 0.25
2.004SerIle: 2.004 ± 0.376
1.469SerLys: 1.469 ± 0.364
3.807SerLeu: 3.807 ± 0.513
1.469SerMet: 1.469 ± 0.299
1.87SerAsn: 1.87 ± 0.445
2.872SerPro: 2.872 ± 0.441
1.803SerGln: 1.803 ± 0.383
3.674SerArg: 3.674 ± 0.5
2.338SerSer: 2.338 ± 0.466
3.74SerThr: 3.74 ± 0.552
4.007SerVal: 4.007 ± 0.436
0.868SerTrp: 0.868 ± 0.231
1.135SerTyr: 1.135 ± 0.219
0.0SerXaa: 0.0 ± 0.0
Thr
6.746ThrAla: 6.746 ± 0.608
0.401ThrCys: 0.401 ± 0.156
3.941ThrAsp: 3.941 ± 0.588
2.872ThrGlu: 2.872 ± 0.414
1.67ThrPhe: 1.67 ± 0.468
5.21ThrGly: 5.21 ± 0.571
1.002ThrHis: 1.002 ± 0.256
4.007ThrIle: 4.007 ± 0.538
2.004ThrLys: 2.004 ± 0.348
5.277ThrLeu: 5.277 ± 0.484
0.868ThrMet: 0.868 ± 0.229
1.469ThrAsn: 1.469 ± 0.305
5.277ThrPro: 5.277 ± 0.578
2.404ThrGln: 2.404 ± 0.37
3.874ThrArg: 3.874 ± 0.427
2.738ThrSer: 2.738 ± 0.397
4.341ThrThr: 4.341 ± 0.495
5.878ThrVal: 5.878 ± 0.645
1.403ThrTrp: 1.403 ± 0.348
1.469ThrTyr: 1.469 ± 0.341
0.0ThrXaa: 0.0 ± 0.0
Val
7.614ValAla: 7.614 ± 0.863
1.069ValCys: 1.069 ± 0.312
5.41ValAsp: 5.41 ± 0.702
4.876ValGlu: 4.876 ± 0.63
2.137ValPhe: 2.137 ± 0.35
5.477ValGly: 5.477 ± 0.755
1.336ValHis: 1.336 ± 0.298
2.071ValIle: 2.071 ± 0.375
1.536ValLys: 1.536 ± 0.319
5.343ValLeu: 5.343 ± 0.588
0.935ValMet: 0.935 ± 0.245
2.538ValAsn: 2.538 ± 0.368
4.408ValPro: 4.408 ± 0.527
1.937ValGln: 1.937 ± 0.343
3.874ValArg: 3.874 ± 0.57
4.141ValSer: 4.141 ± 0.653
5.477ValThr: 5.477 ± 0.67
6.479ValVal: 6.479 ± 0.751
1.269ValTrp: 1.269 ± 0.255
1.269ValTyr: 1.269 ± 0.279
0.0ValXaa: 0.0 ± 0.0
Trp
2.204TrpAla: 2.204 ± 0.413
0.534TrpCys: 0.534 ± 0.164
0.735TrpAsp: 0.735 ± 0.191
0.868TrpGlu: 0.868 ± 0.239
1.269TrpPhe: 1.269 ± 0.307
1.135TrpGly: 1.135 ± 0.251
0.334TrpHis: 0.334 ± 0.169
0.935TrpIle: 0.935 ± 0.257
0.468TrpLys: 0.468 ± 0.153
1.536TrpLeu: 1.536 ± 0.331
0.334TrpMet: 0.334 ± 0.14
0.735TrpAsn: 0.735 ± 0.179
1.069TrpPro: 1.069 ± 0.303
1.269TrpGln: 1.269 ± 0.26
1.603TrpArg: 1.603 ± 0.372
1.135TrpSer: 1.135 ± 0.242
1.069TrpThr: 1.069 ± 0.308
1.135TrpVal: 1.135 ± 0.294
0.601TrpTrp: 0.601 ± 0.193
0.401TrpTyr: 0.401 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.672TyrAla: 2.672 ± 0.436
0.468TyrCys: 0.468 ± 0.174
1.469TyrAsp: 1.469 ± 0.325
1.336TyrGlu: 1.336 ± 0.233
0.601TyrPhe: 0.601 ± 0.203
2.071TyrGly: 2.071 ± 0.41
0.868TyrHis: 0.868 ± 0.205
0.735TyrIle: 0.735 ± 0.27
0.534TyrLys: 0.534 ± 0.189
2.137TyrLeu: 2.137 ± 0.353
0.401TyrMet: 0.401 ± 0.159
0.868TyrAsn: 0.868 ± 0.21
0.935TyrPro: 0.935 ± 0.294
0.935TyrGln: 0.935 ± 0.282
1.737TyrArg: 1.737 ± 0.322
1.403TyrSer: 1.403 ± 0.264
1.67TyrThr: 1.67 ± 0.322
1.403TyrVal: 1.403 ± 0.304
0.401TyrTrp: 0.401 ± 0.147
0.534TyrTyr: 0.534 ± 0.172
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (14973 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski