Nakamurella silvestris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Nakamurellales; Nakamurellaceae; Nakamurella

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4524 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399VQY1|A0A399VQY1_9ACTN Nucleotide-binding protein D1871_00570 OS=Nakamurella silvestris OX=1645681 GN=rapZ PE=3 SV=1
MM1 pKa = 7.83SDD3 pKa = 2.92RR4 pKa = 11.84TVTFNHH10 pKa = 7.15LGNCIINADD19 pKa = 3.59QTGTDD24 pKa = 4.07DD25 pKa = 4.13YY26 pKa = 11.65VAASTKK32 pKa = 7.24TQQIAVGAAAQAISITTPLPDD53 pKa = 3.79APVVGDD59 pKa = 4.49ALDD62 pKa = 3.77LAATGGASGNVVTFSTTTTGICTVTGTTVTFDD94 pKa = 3.38HH95 pKa = 7.21VGDD98 pKa = 4.13CLINADD104 pKa = 3.71QTGTTDD110 pKa = 3.24YY111 pKa = 9.91TAAPTATRR119 pKa = 11.84SVTIGQAPQAISFTTTEE136 pKa = 4.08PDD138 pKa = 3.27TAVVGDD144 pKa = 4.33SYY146 pKa = 11.67EE147 pKa = 4.01VAAVGGALRR156 pKa = 11.84SNPVNRR162 pKa = 11.84SLAADD167 pKa = 4.17GSGEE171 pKa = 4.12PVTFSTTSPEE181 pKa = 3.54ICTVSGSTVTFDD193 pKa = 4.39HH194 pKa = 7.34VGDD197 pKa = 4.13CLINADD203 pKa = 3.86QAGDD207 pKa = 3.95DD208 pKa = 5.11DD209 pKa = 4.46YY210 pKa = 11.59TAAPTIIQTVTVGQAPQAISFTTTEE235 pKa = 3.81PEE237 pKa = 4.02APVVGQTHH245 pKa = 5.61TVAATGGPSGNEE257 pKa = 3.85VTFSTPSAEE266 pKa = 4.03VCTVNGATVTFTHH279 pKa = 7.16PGDD282 pKa = 3.92CVVNADD288 pKa = 3.88QVGNDD293 pKa = 4.8DD294 pKa = 3.77YY295 pKa = 11.39TAAPTTTQTLTIGKK309 pKa = 9.43APQTVEE315 pKa = 4.39FSSTPPSDD323 pKa = 3.89AKK325 pKa = 11.14AGDD328 pKa = 3.6TYY330 pKa = 11.51EE331 pKa = 4.2PVATGGTSGTPVVFSIDD348 pKa = 3.5PAGTPGACEE357 pKa = 3.43IVAGKK362 pKa = 10.35VLFTGPGTCIITAEE376 pKa = 4.03QAGDD380 pKa = 3.9ADD382 pKa = 4.31HH383 pKa = 7.15LPTTSQQTVTVAAGEE398 pKa = 4.25VTITTEE404 pKa = 4.14ALPHH408 pKa = 5.98GAVGLDD414 pKa = 3.42YY415 pKa = 7.66TTSVQAEE422 pKa = 4.45GGLAPYY428 pKa = 9.91AWAVAAGSLPDD439 pKa = 4.89GLALDD444 pKa = 4.96PDD446 pKa = 4.2SGDD449 pKa = 3.05ITGTPTQPGAFTFTISATDD468 pKa = 3.72SEE470 pKa = 4.54NQPVAGTRR478 pKa = 11.84AFTLTVLAEE487 pKa = 4.24EE488 pKa = 4.31VLPPVVDD495 pKa = 4.22PPTGPTGEE503 pKa = 4.3PTTTPVPTTGSTTAVTPGTTTPGAPGTTPPPPTALPATGVNTLPLILLGLVLLVAGLLLNSRR565 pKa = 11.84IGRR568 pKa = 11.84NRR570 pKa = 11.84HH571 pKa = 3.37QHH573 pKa = 4.58

Molecular weight: 57.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399UPE8|A0A399UPE8_9ACTN Beta-N-acetylhexosaminidase OS=Nakamurella silvestris OX=1645681 GN=D1871_21610 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.81TRR25 pKa = 11.84VARR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.68AGKK33 pKa = 9.73

Molecular weight: 4.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4524

0

4524

1536067

27

3932

339.5

36.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.827 ± 0.052

0.701 ± 0.011

5.934 ± 0.029

5.007 ± 0.04

2.954 ± 0.02

9.353 ± 0.035

1.942 ± 0.018

4.299 ± 0.025

2.327 ± 0.026

9.72 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.725 ± 0.017

2.367 ± 0.035

5.787 ± 0.034

2.99 ± 0.018

6.623 ± 0.052

6.051 ± 0.03

7.068 ± 0.074

8.826 ± 0.035

1.433 ± 0.015

2.065 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski