Micromonospora pisi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495JCA4|A0A495JCA4_9ACTN Uncharacterized protein OS=Micromonospora pisi OX=589240 GN=BDK92_0233 PE=4 SV=1
MM1 pKa = 7.19ATYY4 pKa = 10.17HH5 pKa = 6.23IQTEE9 pKa = 4.31DD10 pKa = 3.56MQEE13 pKa = 4.07SASWLQQNMQTLLDD27 pKa = 4.01GMNQAKK33 pKa = 10.44SKK35 pKa = 10.47IDD37 pKa = 3.31TLIQGGYY44 pKa = 6.94NTPAAQAKK52 pKa = 8.61FGPYY56 pKa = 8.82FDD58 pKa = 4.87EE59 pKa = 4.7YY60 pKa = 10.94KK61 pKa = 10.91SSVDD65 pKa = 3.17QTLQGMEE72 pKa = 5.08GISQYY77 pKa = 10.52LQQVGTAFEE86 pKa = 4.46DD87 pKa = 3.8TDD89 pKa = 3.95TQTGNSLGG97 pKa = 3.67

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495JGD3|A0A495JGD3_9ACTN Uncharacterized protein OS=Micromonospora pisi OX=589240 GN=BDK92_2156 PE=4 SV=1
MM1 pKa = 7.84AEE3 pKa = 4.06ALKK6 pKa = 9.74ATSRR10 pKa = 11.84PAAKK14 pKa = 8.96RR15 pKa = 11.84TGVKK19 pKa = 8.77KK20 pKa = 7.29TTAARR25 pKa = 11.84STTGAKK31 pKa = 7.71KK32 pKa = 8.88TPAARR37 pKa = 11.84KK38 pKa = 4.52TTAAAKK44 pKa = 8.78STTTAARR51 pKa = 11.84PTSTRR56 pKa = 11.84ATATRR61 pKa = 11.84PATAKK66 pKa = 9.05KK67 pKa = 7.95TAAKK71 pKa = 9.46KK72 pKa = 10.76APVKK76 pKa = 10.27KK77 pKa = 10.16AAAKK81 pKa = 6.59TTTAKK86 pKa = 9.32KK87 pKa = 8.54TVASKK92 pKa = 10.75AATARR97 pKa = 11.84KK98 pKa = 9.32KK99 pKa = 10.01ATTAATRR106 pKa = 11.84AAGAVKK112 pKa = 8.9KK113 pKa = 8.45TAAKK117 pKa = 9.62KK118 pKa = 10.42APAKK122 pKa = 10.26RR123 pKa = 11.84STAAKK128 pKa = 8.02STPAKK133 pKa = 9.65STTAGSAVKK142 pKa = 8.98KK143 pKa = 9.04TVGAKK148 pKa = 6.46TTAKK152 pKa = 10.2KK153 pKa = 10.68APAKK157 pKa = 10.16KK158 pKa = 9.17ATATRR163 pKa = 11.84STAPGTRR170 pKa = 11.84ATAKK174 pKa = 10.13KK175 pKa = 10.38APAKK179 pKa = 10.27KK180 pKa = 9.3ATTAKK185 pKa = 9.42STTSRR190 pKa = 11.84AATGARR196 pKa = 11.84KK197 pKa = 6.54TTVGAIKK204 pKa = 10.57KK205 pKa = 8.27ATAKK209 pKa = 10.58KK210 pKa = 10.49AGTTTTRR217 pKa = 11.84TPAKK221 pKa = 10.17KK222 pKa = 8.55ITARR226 pKa = 11.84KK227 pKa = 9.49APAKK231 pKa = 10.06KK232 pKa = 9.78VAARR236 pKa = 11.84QAPAKK241 pKa = 9.71KK242 pKa = 8.71STRR245 pKa = 11.84ATVRR249 pKa = 11.84KK250 pKa = 9.29AAGG253 pKa = 3.12

Molecular weight: 25.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7409

0

7409

2538772

29

9546

342.7

36.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.352 ± 0.044

0.699 ± 0.007

5.965 ± 0.023

5.069 ± 0.027

2.704 ± 0.015

9.417 ± 0.03

2.073 ± 0.012

3.402 ± 0.019

1.605 ± 0.019

10.441 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.614 ± 0.01

2.045 ± 0.02

6.37 ± 0.027

2.928 ± 0.017

8.151 ± 0.03

5.079 ± 0.025

6.454 ± 0.028

8.878 ± 0.027

1.613 ± 0.011

2.14 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski