Oryzomicrobium terrae
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C1E555|A0A5C1E555_9RHOO Heptaprenyl diphosphate synthase OS=Oryzomicrobium terrae OX=1735038 GN=hepST PE=4 SV=1
MM1 pKa = 7.63 SPTQPPHH8 pKa = 7.62 DD9 pKa = 4.48 DD10 pKa = 3.19 TAALPDD16 pKa = 3.42 VHH18 pKa = 6.89 EE19 pKa = 5.15 PSLADD24 pKa = 3.02 ILRR27 pKa = 11.84 RR28 pKa = 11.84 SNLSGVYY35 pKa = 10.06 ALPFSGRR42 pKa = 11.84 GGVEE46 pKa = 3.76 HH47 pKa = 7.08 AVAACGHH54 pKa = 6.49 RR55 pKa = 11.84 LLVADD60 pKa = 5.11 CEE62 pKa = 4.3 DD63 pKa = 4.07 SEE65 pKa = 5.24 DD66 pKa = 3.8 VGIVLALLGRR76 pKa = 11.84 DD77 pKa = 3.59 LQLPDD82 pKa = 4.05 YY83 pKa = 11.06 YY84 pKa = 11.06 GANYY88 pKa = 9.26 DD89 pKa = 4.35 ALADD93 pKa = 4.06 CLTDD97 pKa = 3.81 EE98 pKa = 4.9 AWAAPAGGEE107 pKa = 4.11 VAGQVLVIAGADD119 pKa = 3.73 PLKK122 pKa = 11.07 SADD125 pKa = 3.66 PEE127 pKa = 4.53 GFDD130 pKa = 3.49 TLCAVFSAAAEE141 pKa = 4.51 FWRR144 pKa = 11.84 EE145 pKa = 3.49 AGVPFWVFFDD155 pKa = 4.47 LPADD159 pKa = 3.92 GLVDD163 pKa = 5.44 LPILPAA169 pKa = 5.07
Molecular weight: 17.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.872
IPC_protein 3.872
Toseland 3.656
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.24
Thurlkill 3.719
EMBOSS 3.821
Sillero 3.999
Patrickios 1.914
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A5C1E3R1|A0A5C1E3R1_9RHOO PII-like signal transmitter protein OS=Oryzomicrobium terrae OX=1735038 GN=glnK PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.6 RR14 pKa = 11.84 THH16 pKa = 5.75 GFLVRR21 pKa = 11.84 MKK23 pKa = 10.03 TRR25 pKa = 11.84 GGKK28 pKa = 9.44 KK29 pKa = 9.34 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.44 GRR39 pKa = 11.84 HH40 pKa = 4.61 RR41 pKa = 11.84 LAVV44 pKa = 3.37
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3119
0
3119
1042354
29
2274
334.2
36.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.208 ± 0.07
0.887 ± 0.013
5.265 ± 0.03
5.543 ± 0.039
3.541 ± 0.029
8.423 ± 0.038
2.123 ± 0.02
4.36 ± 0.029
3.39 ± 0.043
11.222 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.083 ± 0.02
2.625 ± 0.026
5.495 ± 0.037
3.62 ± 0.027
6.88 ± 0.041
5.05 ± 0.036
5.11 ± 0.033
7.558 ± 0.039
1.334 ± 0.018
2.281 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here