Bacillus endozanthoxylicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Niallia

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5H439|A0A5J5H439_9BACI DUF3920 family protein OS=Bacillus endozanthoxylicus OX=2036016 GN=F4V44_24125 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 9.85PIRR5 pKa = 11.84GSQLTPNNVQEE16 pKa = 4.08EE17 pKa = 5.04CIRR20 pKa = 11.84VPKK23 pKa = 10.44VYY25 pKa = 10.52DD26 pKa = 3.32WVFDD30 pKa = 5.17AITTDD35 pKa = 3.33TGIVLPDD42 pKa = 3.28EE43 pKa = 4.55CAAAVDD49 pKa = 3.96LAVAEE54 pKa = 4.48GRR56 pKa = 11.84TPLDD60 pKa = 3.45VTCEE64 pKa = 4.07VPDD67 pKa = 3.46VGGFFPLDD75 pKa = 3.78PPDD78 pKa = 3.51TDD80 pKa = 4.65GNALCTVSSRR90 pKa = 11.84IEE92 pKa = 3.63RR93 pKa = 11.84RR94 pKa = 11.84EE95 pKa = 3.58IVVNGTPRR103 pKa = 11.84EE104 pKa = 3.79IAIVKK109 pKa = 9.12VIFTIRR115 pKa = 11.84PLITILDD122 pKa = 3.82SEE124 pKa = 4.79GAVICSFRR132 pKa = 11.84PTISEE137 pKa = 3.85SRR139 pKa = 11.84RR140 pKa = 11.84LAVCAPEE147 pKa = 5.14PFTSDD152 pKa = 3.04NVFCRR157 pKa = 11.84IISLNCDD164 pKa = 3.05TNFIEE169 pKa = 5.28TGIPDD174 pKa = 4.24LGLQVMLDD182 pKa = 3.17ICFEE186 pKa = 4.29VQVEE190 pKa = 4.47AEE192 pKa = 4.31VKK194 pKa = 10.88LEE196 pKa = 3.92VLAKK200 pKa = 10.37FCFPRR205 pKa = 11.84EE206 pKa = 3.81NDD208 pKa = 3.12IEE210 pKa = 4.29IPTAGVCPPFEE221 pKa = 4.27WPEE224 pKa = 3.69QCNFFPRR231 pKa = 11.84DD232 pKa = 3.47NCDD235 pKa = 3.04CQAFVDD241 pKa = 4.13TDD243 pKa = 4.92PITGLVPITFDD254 pKa = 4.02ASLFDD259 pKa = 4.12APLAAGSYY267 pKa = 5.82TTEE270 pKa = 4.25LNAEE274 pKa = 3.91ICDD277 pKa = 3.67NCTLAGSTLQWIVEE291 pKa = 4.27DD292 pKa = 4.44FVIPTGTGTGTVDD305 pKa = 3.58QSFTFTAEE313 pKa = 4.05EE314 pKa = 3.96IGMPEE319 pKa = 4.12CTEE322 pKa = 3.89VLGITTMTVEE332 pKa = 4.31GAGTVNFADD341 pKa = 4.22PGVADD346 pKa = 3.87RR347 pKa = 11.84TVLFTLTLVEE357 pKa = 4.93NIGSLDD363 pKa = 3.55DD364 pKa = 4.84AYY366 pKa = 10.75AITLTDD372 pKa = 3.71LAGAPLVTFAGAGVGFVPDD391 pKa = 3.87EE392 pKa = 4.38DD393 pKa = 5.76LVVQDD398 pKa = 5.23CVTFPNLLGDD408 pKa = 3.74TTLLL412 pKa = 3.89

Molecular weight: 44.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5H4J7|A0A5J5H4J7_9BACI Efflux RND transporter periplasmic adaptor subunit OS=Bacillus endozanthoxylicus OX=2036016 GN=F4V44_22900 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSSNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5193

0

5193

1545874

16

2343

297.7

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.886 ± 0.036

0.778 ± 0.01

4.856 ± 0.023

7.576 ± 0.035

4.591 ± 0.032

6.852 ± 0.033

2.119 ± 0.016

8.14 ± 0.029

6.951 ± 0.033

9.841 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.015

4.478 ± 0.02

3.611 ± 0.023

3.768 ± 0.025

3.921 ± 0.023

6.054 ± 0.023

5.329 ± 0.024

6.868 ± 0.03

1.021 ± 0.013

3.539 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski