Escherichia virus P88_4B11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Peduovirus; unclassified Peduovirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FT50|A0A653FT50_9CAUD Uncharacterized protein OS=Escherichia virus P88_4B11 OX=2686060 PE=4 SV=1
MM1 pKa = 7.42CFEE4 pKa = 5.38HH5 pKa = 8.69IEE7 pKa = 4.25DD8 pKa = 4.12LVADD12 pKa = 4.31IAAIFNWSPAEE23 pKa = 3.86IFMMTPGEE31 pKa = 4.27VVSWRR36 pKa = 11.84EE37 pKa = 3.52RR38 pKa = 11.84AALRR42 pKa = 11.84SGNADD47 pKa = 3.47NEE49 pKa = 4.46DD50 pKa = 3.64SS51 pKa = 3.73

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FSL4|A0A653FSL4_9CAUD Uncharacterized protein OS=Escherichia virus P88_4B11 OX=2686060 PE=4 SV=1
MM1 pKa = 7.41LQPSHH6 pKa = 7.55VNVLKK11 pKa = 10.72QLVGAEE17 pKa = 3.97VGLICVRR24 pKa = 11.84RR25 pKa = 11.84AVEE28 pKa = 3.96TDD30 pKa = 2.5HH31 pKa = 7.55DD32 pKa = 4.16AVKK35 pKa = 10.63RR36 pKa = 11.84HH37 pKa = 5.87LPGNLCADD45 pKa = 4.19AGLKK49 pKa = 9.9IRR51 pKa = 11.84VCRR54 pKa = 11.84PGIKK58 pKa = 10.04LRR60 pKa = 11.84IRR62 pKa = 11.84NKK64 pKa = 9.89RR65 pKa = 11.84VVVHH69 pKa = 6.3LFALVFAVFQINHH82 pKa = 6.63VIRR85 pKa = 11.84VTAFCAVITGIRR97 pKa = 11.84ARR99 pKa = 11.84NRR101 pKa = 11.84PGGVQEE107 pKa = 5.19DD108 pKa = 4.25FFAFLVGHH116 pKa = 5.69TEE118 pKa = 3.99HH119 pKa = 7.29VNFFCKK125 pKa = 10.39FSALLYY131 pKa = 10.54CVFKK135 pKa = 10.44TLLHH139 pKa = 6.48RR140 pKa = 11.84GFHH143 pKa = 5.82GKK145 pKa = 9.03SRR147 pKa = 11.84YY148 pKa = 5.59QCRR151 pKa = 11.84YY152 pKa = 7.68RR153 pKa = 11.84DD154 pKa = 3.62VVKK157 pKa = 10.63RR158 pKa = 11.84RR159 pKa = 11.84LLRR162 pKa = 11.84HH163 pKa = 6.13IATQLVAGNII173 pKa = 3.82

Molecular weight: 19.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11168

35

940

199.4

22.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.76 ± 0.576

1.083 ± 0.172

5.802 ± 0.252

6.223 ± 0.321

3.528 ± 0.205

6.922 ± 0.324

1.934 ± 0.213

5.614 ± 0.293

5.113 ± 0.284

8.847 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.154

4.182 ± 0.235

3.922 ± 0.183

4.092 ± 0.177

7.119 ± 0.364

6.268 ± 0.284

6.725 ± 0.368

6.062 ± 0.303

1.603 ± 0.146

2.624 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski