Aureobasidium melanogenum CBS 110374
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10583 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A074VG94|A0A074VG94_9PEZI Uncharacterized protein OS=Aureobasidium melanogenum CBS 110374 OX=1043003 GN=M437DRAFT_55826 PE=4 SV=1
MM1 pKa = 7.61 HH2 pKa = 7.49 NFTIQATLVFFTLGTISTVASPADD26 pKa = 3.6 MLTALMLPSLLSKK39 pKa = 10.36 PDD41 pKa = 3.26 VSIQTLCSGAEE52 pKa = 4.19 GNTTADD58 pKa = 4.34 DD59 pKa = 3.71 STTAAKK65 pKa = 10.1 IISSGNKK72 pKa = 8.97 EE73 pKa = 4.17 DD74 pKa = 3.76 NQAALNTQPSYY85 pKa = 11.48 CPDD88 pKa = 3.33 SYY90 pKa = 11.05 EE91 pKa = 4.13 TMPMCVDD98 pKa = 3.79 CGGFTLFYY106 pKa = 10.56 YY107 pKa = 10.64 KK108 pKa = 10.6 EE109 pKa = 3.81 LDD111 pKa = 3.99 YY112 pKa = 11.67 YY113 pKa = 9.78 DD114 pKa = 4.07 TRR116 pKa = 11.84 CKK118 pKa = 10.69 GVGDD122 pKa = 3.93 NEE124 pKa = 4.16 DD125 pKa = 5.23 LKK127 pKa = 11.41 DD128 pKa = 3.89 CSCLEE133 pKa = 3.62 RR134 pKa = 11.84 RR135 pKa = 11.84 YY136 pKa = 10.28 QEE138 pKa = 5.44 DD139 pKa = 3.91 DD140 pKa = 5.21 DD141 pKa = 4.55 IVCNMFAADD150 pKa = 4.56 PDD152 pKa = 3.79 IDD154 pKa = 4.67 PDD156 pKa = 3.92 PEE158 pKa = 3.68 ATRR161 pKa = 11.84 RR162 pKa = 11.84 KK163 pKa = 7.56 TVEE166 pKa = 3.91 AMIQVPDD173 pKa = 4.52 SIIDD177 pKa = 4.53 PITGTEE183 pKa = 4.06 LNQPHH188 pKa = 6.76 IVLDD192 pKa = 4.0 DD193 pKa = 4.51 DD194 pKa = 4.38 FWAATVQEE202 pKa = 4.92 IMDD205 pKa = 3.94 QEE207 pKa = 4.5 DD208 pKa = 3.78 KK209 pKa = 11.04 EE210 pKa = 4.59 QEE212 pKa = 4.16 DD213 pKa = 3.76 EE214 pKa = 4.41 CLPKK218 pKa = 10.76 EE219 pKa = 4.48 SVV221 pKa = 3.02
Molecular weight: 24.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.948
Patrickios 1.214
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A074VTW3|A0A074VTW3_9PEZI Uncharacterized protein OS=Aureobasidium melanogenum CBS 110374 OX=1043003 GN=M437DRAFT_67501 PE=4 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TNNTIRR36 pKa = 11.84 YY37 pKa = 5.73 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.15 WRR45 pKa = 11.84 KK46 pKa = 7.38 TRR48 pKa = 11.84 IGII51 pKa = 4.0
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10583
0
10583
4696349
49
4913
443.8
49.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.825 ± 0.022
1.268 ± 0.008
5.803 ± 0.02
6.08 ± 0.02
3.73 ± 0.015
6.478 ± 0.023
2.416 ± 0.011
4.939 ± 0.018
5.074 ± 0.023
8.845 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.25 ± 0.009
3.867 ± 0.013
5.751 ± 0.025
4.198 ± 0.018
5.766 ± 0.022
8.247 ± 0.035
6.085 ± 0.018
6.075 ± 0.017
1.46 ± 0.008
2.843 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here