Rufibacter sp. DG15C

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Rufibacter; unclassified Rufibacter

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127B3H7|A0A127B3H7_9BACT Aminopeptidase OS=Rufibacter sp. DG15C OX=1379909 GN=TH61_12595 PE=4 SV=1
MM1 pKa = 6.89FQINAHH7 pKa = 5.56VLPSIDD13 pKa = 3.1SSQFGKK19 pKa = 10.68AQGAYY24 pKa = 9.63AVIFIDD30 pKa = 4.45YY31 pKa = 10.39KK32 pKa = 11.32DD33 pKa = 3.3IDD35 pKa = 3.83GAYY38 pKa = 10.03ALAKK42 pKa = 9.93FYY44 pKa = 10.68IEE46 pKa = 4.45DD47 pKa = 4.43DD48 pKa = 3.38GWVIDD53 pKa = 4.79EE54 pKa = 4.68IQDD57 pKa = 3.38EE58 pKa = 4.71YY59 pKa = 12.06LLFTSPEE66 pKa = 4.2DD67 pKa = 3.49VSEE70 pKa = 4.51EE71 pKa = 3.69YY72 pKa = 10.81LPYY75 pKa = 10.5YY76 pKa = 10.55KK77 pKa = 10.37EE78 pKa = 4.06ALEE81 pKa = 4.23TGYY84 pKa = 11.4SLLFNCYY91 pKa = 9.95EE92 pKa = 4.46KK93 pKa = 11.04DD94 pKa = 3.74DD95 pKa = 4.9LKK97 pKa = 11.39DD98 pKa = 3.18

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127B3K1|A0A127B3K1_9BACT Uncharacterized protein OS=Rufibacter sp. DG15C OX=1379909 GN=TH61_07650 PE=4 SV=1
MM1 pKa = 7.65AKK3 pKa = 10.4AFATSTQSSLSDD15 pKa = 3.36EE16 pKa = 4.5SPKK19 pKa = 10.5QVAASGRR26 pKa = 11.84TAVRR30 pKa = 11.84ARR32 pKa = 11.84QDD34 pKa = 3.11WLISRR39 pKa = 11.84GWRR42 pKa = 11.84CAEE45 pKa = 3.73EE46 pKa = 4.14ASWYY50 pKa = 8.65SARR53 pKa = 11.84RR54 pKa = 11.84EE55 pKa = 4.16DD56 pKa = 3.93GAPRR60 pKa = 11.84PRR62 pKa = 11.84ALSGEE67 pKa = 4.27NEE69 pKa = 4.44TIQHH73 pKa = 6.49KK74 pKa = 9.11GTGRR78 pKa = 11.84KK79 pKa = 8.87NLLRR83 pKa = 11.84NSQLRR88 pKa = 11.84AQCVAISKK96 pKa = 10.14SVV98 pKa = 2.76

Molecular weight: 10.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3169

0

3169

1046099

58

5701

330.1

36.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.068 ± 0.048

0.724 ± 0.017

4.996 ± 0.033

6.249 ± 0.052

4.685 ± 0.037

6.949 ± 0.042

2.056 ± 0.023

5.922 ± 0.038

6.223 ± 0.053

10.202 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.021

4.553 ± 0.036

4.215 ± 0.028

4.608 ± 0.039

4.29 ± 0.035

6.006 ± 0.04

6.015 ± 0.065

6.985 ± 0.037

1.138 ± 0.02

3.735 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski