Rice black streaked dwarf virus (RBSDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Fijivirus

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4IX43|R4IX43_RBSDV p8 OS=Rice black streaked dwarf virus OX=10990 PE=4 SV=1
MM1 pKa = 7.37NPQSSVNVDD10 pKa = 3.39TYY12 pKa = 11.36TFNCPFEE19 pKa = 3.96LAKK22 pKa = 10.44IQIEE26 pKa = 4.34SMKK29 pKa = 10.41PIMQDD34 pKa = 2.87FSNFDD39 pKa = 4.28DD40 pKa = 3.66IFNRR44 pKa = 11.84ALSDD48 pKa = 3.68SEE50 pKa = 3.69IDD52 pKa = 4.07DD53 pKa = 3.46RR54 pKa = 11.84VEE56 pKa = 3.69QLEE59 pKa = 4.04VDD61 pKa = 4.1VEE63 pKa = 4.75SKK65 pKa = 10.64VDD67 pKa = 4.12PIVRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84YY74 pKa = 10.0GKK76 pKa = 9.49IGHH79 pKa = 7.08IIIMIISFVFFGIFKK94 pKa = 9.18LTLKK98 pKa = 9.96MFYY101 pKa = 10.36HH102 pKa = 7.61LFRR105 pKa = 11.84CVCCNPLIRR114 pKa = 11.84GILSIIFTILFYY126 pKa = 10.96FLLCVCIYY134 pKa = 9.76FVYY137 pKa = 10.79YY138 pKa = 10.15FFGDD142 pKa = 4.0QISLAYY148 pKa = 8.5NTLNQIDD155 pKa = 3.91SSNFINSTQVEE166 pKa = 4.48EE167 pKa = 4.08KK168 pKa = 10.15VKK170 pKa = 11.07NIIHH174 pKa = 6.96DD175 pKa = 3.79GSLFFGTRR183 pKa = 11.84DD184 pKa = 3.24QATGQINEE192 pKa = 4.45IEE194 pKa = 4.35TQVVNGGTVNYY205 pKa = 9.68TLFNN209 pKa = 4.17

Molecular weight: 24.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4TM77|S4TM77_RBSDV p3 OS=Rice black streaked dwarf virus OX=10990 PE=4 SV=1
MM1 pKa = 7.52TKK3 pKa = 10.27FPLVSQRR10 pKa = 11.84NKK12 pKa = 9.84MGCSHH17 pKa = 6.17QKK19 pKa = 10.28KK20 pKa = 9.46CVCLILSEE28 pKa = 4.95NLADD32 pKa = 3.92WEE34 pKa = 4.32LTMTTVNCQMLSHH47 pKa = 6.22QGKK50 pKa = 9.56NYY52 pKa = 6.54TTIIPVRR59 pKa = 11.84CDD61 pKa = 3.37SEE63 pKa = 4.37ISDD66 pKa = 4.09ILSSANILDD75 pKa = 3.52VRR77 pKa = 11.84HH78 pKa = 5.51VVTITEE84 pKa = 4.15LASNFPCVNWDD95 pKa = 3.53IKK97 pKa = 10.44RR98 pKa = 11.84QQLKK102 pKa = 10.44RR103 pKa = 11.84YY104 pKa = 9.89DD105 pKa = 3.64PTQLLVNLEE114 pKa = 4.05QNFNHH119 pKa = 6.93LQRR122 pKa = 11.84DD123 pKa = 3.44DD124 pKa = 3.93RR125 pKa = 11.84LCFNSCYY132 pKa = 10.37DD133 pKa = 3.88HH134 pKa = 7.23PNSHH138 pKa = 7.31IINRR142 pKa = 11.84ILNTKK147 pKa = 9.86KK148 pKa = 10.65DD149 pKa = 3.88VIPFIFKK156 pKa = 10.46HH157 pKa = 5.99YY158 pKa = 10.35DD159 pKa = 3.23DD160 pKa = 5.49SNSKK164 pKa = 10.57SIADD168 pKa = 3.02INVFNSPSYY177 pKa = 9.89SKK179 pKa = 10.87HH180 pKa = 5.18VFEE183 pKa = 4.92KK184 pKa = 10.37TNIIKK189 pKa = 8.95TLLFGSTLGVMLHH202 pKa = 6.6LL203 pKa = 5.04

Molecular weight: 23.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

9313

203

1464

716.4

82.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.941 ± 0.255

1.589 ± 0.134

6.185 ± 0.203

5.659 ± 0.277

6.292 ± 0.291

3.79 ± 0.274

2.459 ± 0.163

7.055 ± 0.273

6.722 ± 0.294

10.169 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.121

7.098 ± 0.339

3.372 ± 0.213

3.243 ± 0.188

4.134 ± 0.224

9.084 ± 0.625

5.616 ± 0.517

6.206 ± 0.221

0.741 ± 0.085

4.241 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski