Drosophila melanogaster birnavirus SW-2009a

Taxonomy: Viruses; Riboviria; Orthornavirae; Birnaviridae; unclassified Birnaviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0U495|D0U495_9VIRU Structural polyprotein OS=Drosophila melanogaster birnavirus SW-2009a OX=663281 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84EE3 pKa = 3.67RR4 pKa = 11.84YY5 pKa = 9.55YY6 pKa = 11.03EE7 pKa = 4.05DD8 pKa = 3.7TEE10 pKa = 4.58LNNLSYY16 pKa = 10.96SEE18 pKa = 3.94QDD20 pKa = 3.13KK21 pKa = 10.66QLKK24 pKa = 9.42QICQTSTLDD33 pKa = 3.21QSSCLRR39 pKa = 11.84EE40 pKa = 4.07VPPVYY45 pKa = 9.42QTTTSAVIAYY55 pKa = 7.86DD56 pKa = 3.49KK57 pKa = 10.78KK58 pKa = 10.79QSLRR62 pKa = 11.84TLWSDD67 pKa = 3.73LLEE70 pKa = 4.75KK71 pKa = 11.05GPLFCSLTILAALLALILSMMTRR94 pKa = 11.84ANLTNTPNHH103 pKa = 6.2SLSPNASMSPIITEE117 pKa = 4.01EE118 pKa = 4.22SPQEE122 pKa = 4.35SYY124 pKa = 10.77TSEE127 pKa = 4.26VPHH130 pKa = 6.06SHH132 pKa = 6.12QEE134 pKa = 3.96STTSQEE140 pKa = 3.59QSMPPRR146 pKa = 11.84MKK148 pKa = 10.53AHH150 pKa = 7.13LRR152 pKa = 11.84RR153 pKa = 3.99

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0U496|D0U496_9VIRU Protein VP1 OS=Drosophila melanogaster birnavirus SW-2009a OX=663281 PE=4 SV=1
MM1 pKa = 7.69NEE3 pKa = 3.92TKK5 pKa = 10.42LVNSTRR11 pKa = 11.84VLEE14 pKa = 4.07IAQYY18 pKa = 8.01IAKK21 pKa = 10.01RR22 pKa = 11.84RR23 pKa = 11.84IHH25 pKa = 6.53AFKK28 pKa = 10.32PYY30 pKa = 10.47YY31 pKa = 10.15LRR33 pKa = 11.84QNLEE37 pKa = 4.09FEE39 pKa = 4.52ASSVLDD45 pKa = 3.91EE46 pKa = 4.32QIVRR50 pKa = 11.84YY51 pKa = 8.78LQTIRR56 pKa = 11.84TLQLEE61 pKa = 4.28SGNPKK66 pKa = 10.46DD67 pKa = 5.62YY68 pKa = 11.22EE69 pKa = 4.51DD70 pKa = 3.78CTKK73 pKa = 10.53IHH75 pKa = 6.8LPDD78 pKa = 5.03GDD80 pKa = 4.17PKK82 pKa = 10.83IPFYY86 pKa = 10.78RR87 pKa = 11.84PRR89 pKa = 11.84FTTGTYY95 pKa = 10.44DD96 pKa = 4.71DD97 pKa = 4.78KK98 pKa = 11.44LLNAIAFQWLASKK111 pKa = 7.7EE112 pKa = 4.41TQGNFEE118 pKa = 4.06TAKK121 pKa = 10.38GYY123 pKa = 9.83VVQRR127 pKa = 11.84DD128 pKa = 4.41RR129 pKa = 11.84IADD132 pKa = 3.78PTDD135 pKa = 2.9MDD137 pKa = 3.97TTIKK141 pKa = 10.35RR142 pKa = 11.84CRR144 pKa = 11.84QACIDD149 pKa = 3.69YY150 pKa = 10.08RR151 pKa = 11.84LSSGTWQGQLARR163 pKa = 11.84LKK165 pKa = 10.62DD166 pKa = 3.69MQNIRR171 pKa = 11.84HH172 pKa = 5.5GVDD175 pKa = 3.24GKK177 pKa = 10.52FLPKK181 pKa = 9.96SLKK184 pKa = 9.95EE185 pKa = 3.8LGYY188 pKa = 10.82DD189 pKa = 3.54LEE191 pKa = 4.54SFSKK195 pKa = 10.42ILEE198 pKa = 3.92KK199 pKa = 10.14TLPINAVDD207 pKa = 4.35LSQTISLRR215 pKa = 11.84YY216 pKa = 9.74LPTLFIEE223 pKa = 4.38EE224 pKa = 4.46TEE226 pKa = 4.36VEE228 pKa = 4.39VKK230 pKa = 10.33GVKK233 pKa = 8.41KK234 pKa = 9.28TSYY237 pKa = 10.55IDD239 pKa = 3.31NTPKK243 pKa = 10.78LKK245 pKa = 10.01PDD247 pKa = 3.64SKK249 pKa = 10.91AGPPFQSKK257 pKa = 8.22YY258 pKa = 10.26RR259 pKa = 11.84KK260 pKa = 9.66GDD262 pKa = 3.37MFSSILALADD272 pKa = 3.82DD273 pKa = 4.77ALLGLSEE280 pKa = 4.58VIHH283 pKa = 6.82GRR285 pKa = 11.84MDD287 pKa = 2.96RR288 pKa = 11.84HH289 pKa = 6.5KK290 pKa = 9.81YY291 pKa = 9.63FKK293 pKa = 10.77DD294 pKa = 3.39RR295 pKa = 11.84IYY297 pKa = 11.27LAMCFLFPKK306 pKa = 9.17EE307 pKa = 3.98EE308 pKa = 4.09RR309 pKa = 11.84YY310 pKa = 9.24EE311 pKa = 4.0ASKK314 pKa = 9.56LTTKK318 pKa = 9.24TRR320 pKa = 11.84NIASVCGVTHH330 pKa = 6.31YY331 pKa = 11.69LMLHH335 pKa = 5.33AMEE338 pKa = 4.88PVMEE342 pKa = 4.18KK343 pKa = 10.76SKK345 pKa = 9.61WNCMSSEE352 pKa = 4.29TYY354 pKa = 10.42EE355 pKa = 4.12NPSLSKK361 pKa = 8.81FTPYY365 pKa = 10.34FGNLQKK371 pKa = 10.86FLDD374 pKa = 4.24FGLHH378 pKa = 5.52RR379 pKa = 11.84EE380 pKa = 4.04KK381 pKa = 10.93KK382 pKa = 9.57KK383 pKa = 11.04VFMPRR388 pKa = 11.84GAKK391 pKa = 7.31PTHH394 pKa = 6.81RR395 pKa = 11.84DD396 pKa = 3.87FIYY399 pKa = 10.71ADD401 pKa = 2.81NWYY404 pKa = 9.85IFYY407 pKa = 10.6RR408 pKa = 11.84NEE410 pKa = 4.88DD411 pKa = 3.47GTTDD415 pKa = 3.84YY416 pKa = 11.68YY417 pKa = 11.72SLDD420 pKa = 3.82LEE422 pKa = 4.53KK423 pKa = 11.37GEE425 pKa = 5.34ANATPDD431 pKa = 2.94IAQCISYY438 pKa = 10.82YY439 pKa = 10.73LLTRR443 pKa = 11.84GWISDD448 pKa = 3.59DD449 pKa = 4.29GIVGFGPTWSTFLTQIVPACIVDD472 pKa = 4.19NISVYY477 pKa = 11.46YY478 pKa = 10.73NMQLRR483 pKa = 11.84FPGQASGNTYY493 pKa = 9.32TFLINHH499 pKa = 7.11AVTTKK504 pKa = 10.42LRR506 pKa = 11.84QEE508 pKa = 4.05WLNRR512 pKa = 11.84GCPRR516 pKa = 11.84PGSAEE521 pKa = 3.67FTQCCKK527 pKa = 9.98KK528 pKa = 9.46TGVNTKK534 pKa = 9.91IEE536 pKa = 4.14FHH538 pKa = 6.53SEE540 pKa = 3.56NLEE543 pKa = 4.03QQVKK547 pKa = 7.53EE548 pKa = 4.69TIANTPNVGFLQGDD562 pKa = 3.78STDD565 pKa = 3.79SVDD568 pKa = 5.41ANGQSWFQKK577 pKa = 10.31ALPRR581 pKa = 11.84LKK583 pKa = 10.55LDD585 pKa = 3.56LLGYY589 pKa = 10.03DD590 pKa = 3.66AVYY593 pKa = 10.72SHH595 pKa = 6.4QLGIFIPVLAEE606 pKa = 3.49EE607 pKa = 4.35RR608 pKa = 11.84FYY610 pKa = 10.92PSMMWPRR617 pKa = 11.84KK618 pKa = 9.58GGEE621 pKa = 4.11TIKK624 pKa = 10.89DD625 pKa = 3.49YY626 pKa = 10.9IQRR629 pKa = 11.84NLYY632 pKa = 9.72NLVRR636 pKa = 11.84YY637 pKa = 8.12EE638 pKa = 3.98VLRR641 pKa = 11.84IMGGWTIPLADD652 pKa = 3.54QALHH656 pKa = 6.58NMATNIRR663 pKa = 11.84TNIVDD668 pKa = 4.12KK669 pKa = 10.26RR670 pKa = 11.84HH671 pKa = 6.29KK672 pKa = 9.89MDD674 pKa = 2.9TSTFLGQEE682 pKa = 4.04DD683 pKa = 4.86LAPDD687 pKa = 3.81LWGAMEE693 pKa = 5.26DD694 pKa = 3.55SGLTTEE700 pKa = 5.24LALNPEE706 pKa = 4.64FSMTHH711 pKa = 5.57EE712 pKa = 4.42LLTRR716 pKa = 11.84LNTPKK721 pKa = 9.08QTSPEE726 pKa = 3.94HH727 pKa = 6.1MPVKK731 pKa = 10.12PKK733 pKa = 9.86PRR735 pKa = 11.84HH736 pKa = 4.71LSKK739 pKa = 10.91PEE741 pKa = 3.77LMADD745 pKa = 3.5LQEE748 pKa = 4.51LKK750 pKa = 10.77KK751 pKa = 11.03SLTNLTSSHH760 pKa = 5.29TEE762 pKa = 3.48KK763 pKa = 11.08LLAKK767 pKa = 9.86PEE769 pKa = 4.09YY770 pKa = 10.07VRR772 pKa = 11.84SKK774 pKa = 10.76LEE776 pKa = 3.89EE777 pKa = 4.1VIKK780 pKa = 9.49TFPSLEE786 pKa = 4.26AIITIMGKK794 pKa = 9.98YY795 pKa = 9.07EE796 pKa = 4.03DD797 pKa = 5.18LYY799 pKa = 11.51SEE801 pKa = 4.75LDD803 pKa = 3.25EE804 pKa = 4.83VYY806 pKa = 10.58NNKK809 pKa = 9.0MKK811 pKa = 10.57IEE813 pKa = 4.28DD814 pKa = 3.86FMDD817 pKa = 5.25KK818 pKa = 9.45LTHH821 pKa = 7.26WINQAQHH828 pKa = 5.33EE829 pKa = 4.66MEE831 pKa = 3.84EE832 pKa = 4.25AMRR835 pKa = 11.84KK836 pKa = 9.33SIRR839 pKa = 11.84VVQQAQVQDD848 pKa = 3.93YY849 pKa = 9.93EE850 pKa = 4.52NIEE853 pKa = 3.88AFYY856 pKa = 10.93EE857 pKa = 4.1KK858 pKa = 10.65DD859 pKa = 3.24PTKK862 pKa = 10.7LADD865 pKa = 3.53PLGYY869 pKa = 10.26KK870 pKa = 10.15GAQTLVGKK878 pKa = 8.49HH879 pKa = 5.41TFPEE883 pKa = 4.43SSSTIPASLTKK894 pKa = 10.28SQKK897 pKa = 10.57KK898 pKa = 9.08NLKK901 pKa = 9.19KK902 pKa = 10.14KK903 pKa = 10.08QRR905 pKa = 11.84ISKK908 pKa = 10.14LRR910 pKa = 11.84DD911 pKa = 2.81IEE913 pKa = 4.14QASWSRR919 pKa = 11.84DD920 pKa = 3.16ALGEE924 pKa = 4.0IRR926 pKa = 11.84KK927 pKa = 9.41HH928 pKa = 6.43EE929 pKa = 4.23EE930 pKa = 3.74DD931 pKa = 3.5LL932 pKa = 4.52

Molecular weight: 107.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2064

153

979

688.0

77.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.444 ± 0.605

0.969 ± 0.242

5.378 ± 0.444

5.959 ± 0.818

2.665 ± 0.746

5.959 ± 1.208

1.89 ± 0.336

6.541 ± 0.774

6.153 ± 1.126

8.915 ± 0.998

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.278

5.039 ± 0.589

6.105 ± 0.667

4.36 ± 0.532

5.087 ± 0.05

7.219 ± 1.132

7.897 ± 0.454

5.329 ± 0.986

0.969 ± 0.24

4.506 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski