Podoviridae sp. ctcf755
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345BNS4|A0A345BNS4_9CAUD Uncharacterized protein OS=Podoviridae sp. ctcf755 OX=2675444 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.38 NLEE5 pKa = 3.96 EE6 pKa = 5.16 SILFLEE12 pKa = 4.51 KK13 pKa = 9.44 RR14 pKa = 11.84 DD15 pKa = 3.63 YY16 pKa = 10.28 TVWIEE21 pKa = 4.99 KK22 pKa = 10.16 DD23 pKa = 3.24 RR24 pKa = 11.84 QTCSVAYY31 pKa = 9.06 PRR33 pKa = 11.84 QFGNYY38 pKa = 8.92 AFIIIADD45 pKa = 3.98 GEE47 pKa = 4.63 KK48 pKa = 10.22 IPSLKK53 pKa = 10.26 TNDD56 pKa = 3.64 EE57 pKa = 3.89 KK58 pKa = 11.56 DD59 pKa = 3.67 VIRR62 pKa = 11.84 FAFDD66 pKa = 2.72 LWDD69 pKa = 5.05 DD70 pKa = 5.0 EE71 pKa = 4.75 IQMTMDD77 pKa = 4.59 DD78 pKa = 3.82 NNNYY82 pKa = 9.22 IFQLSHH88 pKa = 7.12 DD89 pKa = 4.07 VYY91 pKa = 10.68 KK92 pKa = 10.78 AHH94 pKa = 7.7 RR95 pKa = 11.84 YY96 pKa = 9.49 CEE98 pKa = 4.11 SDD100 pKa = 4.16 CIEE103 pKa = 4.25 EE104 pKa = 4.13 PKK106 pKa = 10.74 GFCGDD111 pKa = 3.58 STEE114 pKa = 4.07 ITYY117 pKa = 10.83 KK118 pKa = 10.68 DD119 pKa = 3.28 YY120 pKa = 11.51 VYY122 pKa = 11.18 AKK124 pKa = 9.33 EE125 pKa = 4.23 HH126 pKa = 6.18 EE127 pKa = 4.49 LNFDD131 pKa = 3.19 EE132 pKa = 4.68 MEE134 pKa = 4.24 YY135 pKa = 11.02 GEE137 pKa = 4.23 EE138 pKa = 3.99 RR139 pKa = 11.84 EE140 pKa = 4.03 IYY142 pKa = 10.35 LYY144 pKa = 10.28 TFSIADD150 pKa = 3.73 DD151 pKa = 4.52 DD152 pKa = 4.72 GDD154 pKa = 4.07 YY155 pKa = 11.1 KK156 pKa = 10.8 IVDD159 pKa = 3.27 QFYY162 pKa = 10.71 SFEE165 pKa = 4.24 KK166 pKa = 10.92 VFF168 pKa = 3.79
Molecular weight: 20.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.051
IPC2_protein 4.24
IPC_protein 4.202
Toseland 4.024
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.062
Rodwell 4.037
Grimsley 3.923
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.469
Thurlkill 4.037
EMBOSS 4.075
Sillero 4.317
Patrickios 2.626
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.226
Protein with the highest isoelectric point:
>tr|A0A345BNU5|A0A345BNU5_9CAUD Uncharacterized protein OS=Podoviridae sp. ctcf755 OX=2675444 PE=4 SV=1
MM1 pKa = 7.5 NNPTMMVQQLIRR13 pKa = 11.84 QGLNPNQIMQRR24 pKa = 11.84 FEE26 pKa = 3.73 QMAANNPQIRR36 pKa = 11.84 AALQQMKK43 pKa = 9.73 QAQNQMRR50 pKa = 11.84 QSGMTTQQFAMQYY63 pKa = 10.62 AKK65 pKa = 10.75 QNNINLNPYY74 pKa = 9.18 VQMFNQSGINLL85 pKa = 3.74
Molecular weight: 9.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 10.248
IPC_protein 11.155
Toseland 11.096
ProMoST 11.155
Dawson 11.169
Bjellqvist 10.994
Wikipedia 11.491
Rodwell 11.242
Grimsley 11.228
Solomon 11.433
Lehninger 11.374
Nozaki 11.067
DTASelect 10.994
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.111
Patrickios 11.082
IPC_peptide 11.433
IPC2_peptide 10.189
IPC2.peptide.svr19 8.073
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
11708
80
990
243.9
28.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.903 ± 0.324
1.486 ± 0.226
6.141 ± 0.248
8.362 ± 0.358
4.296 ± 0.187
5.048 ± 0.329
1.153 ± 0.129
9.113 ± 0.401
9.455 ± 0.517
8.165 ± 0.3
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.204 ± 0.169
7.619 ± 0.294
2.417 ± 0.188
3.98 ± 0.304
3.476 ± 0.245
5.714 ± 0.354
5.355 ± 0.382
5.056 ± 0.213
1.076 ± 0.158
4.98 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here