Atkinsonella hypoxylon virus (isolate 2H) (AhV)
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q85056|CAPSD_AHV2H Capsid protein OS=Atkinsonella hypoxylon virus (isolate 2H) OX=647331 PE=3 SV=1
MM1 pKa = 7.61 SSNDD5 pKa = 3.3 SAQTRR10 pKa = 11.84 NLQEE14 pKa = 3.52 EE15 pKa = 4.73 RR16 pKa = 11.84 FNEE19 pKa = 4.26 RR20 pKa = 11.84 TSTPTVVTAVLPDD33 pKa = 3.8 TNGPTTNSTSGSVGPPHH50 pKa = 6.86 PTPNVPVPTQSSSDD64 pKa = 3.65 PPSASGIFAKK74 pKa = 10.69 EE75 pKa = 2.99 IDD77 pKa = 3.8 LPRR80 pKa = 11.84 NVIQHH85 pKa = 6.2 SGNKK89 pKa = 9.7 FILDD93 pKa = 3.63 VVPDD97 pKa = 3.56 SRR99 pKa = 11.84 FPTFAITEE107 pKa = 4.32 FVQRR111 pKa = 11.84 SFSNFTFEE119 pKa = 3.95 QYY121 pKa = 11.06 SYY123 pKa = 11.35 VSPASLVGYY132 pKa = 8.97 LVYY135 pKa = 10.08 MIHH138 pKa = 7.07 AFVFLVDD145 pKa = 3.53 AFEE148 pKa = 4.54 RR149 pKa = 11.84 SPMSAYY155 pKa = 10.26 ASEE158 pKa = 4.28 IDD160 pKa = 3.76 ASHH163 pKa = 6.97 AYY165 pKa = 10.24 LRR167 pKa = 11.84 IIDD170 pKa = 4.53 AFSDD174 pKa = 3.74 AYY176 pKa = 10.1 IPDD179 pKa = 3.93 FLFEE183 pKa = 4.6 ILDD186 pKa = 3.91 TYY188 pKa = 11.0 LSHH191 pKa = 7.69 RR192 pKa = 11.84 LDD194 pKa = 3.21 IRR196 pKa = 11.84 SKK198 pKa = 11.05 LEE200 pKa = 3.64 MNVSYY205 pKa = 11.17 GSVLYY210 pKa = 10.44 KK211 pKa = 10.31 YY212 pKa = 9.26 DD213 pKa = 3.33 APRR216 pKa = 11.84 IVAPSIFLLAHH227 pKa = 5.99 NQLISQSRR235 pKa = 11.84 EE236 pKa = 3.35 STAYY240 pKa = 9.81 EE241 pKa = 3.76 KK242 pKa = 10.42 WLDD245 pKa = 3.91 SIVIHH250 pKa = 6.23 YY251 pKa = 9.5 SRR253 pKa = 11.84 AVIRR257 pKa = 11.84 VGNLVGGLYY266 pKa = 10.04 QSSHH270 pKa = 6.2 GSTTTHH276 pKa = 4.98 FTYY279 pKa = 10.71 RR280 pKa = 11.84 NWFARR285 pKa = 11.84 SLSRR289 pKa = 11.84 LADD292 pKa = 3.5 SATHH296 pKa = 5.66 RR297 pKa = 11.84 THH299 pKa = 7.14 LRR301 pKa = 11.84 RR302 pKa = 11.84 PMISEE307 pKa = 4.26 FDD309 pKa = 3.77 YY310 pKa = 11.38 NIPSVNNNTYY320 pKa = 10.54 NPYY323 pKa = 10.1 VHH325 pKa = 7.56 LLMLEE330 pKa = 4.09 PNNRR334 pKa = 11.84 NITLDD339 pKa = 4.09 FIRR342 pKa = 11.84 SLSSFCSTEE351 pKa = 3.83 LKK353 pKa = 9.57 ATRR356 pKa = 11.84 TLRR359 pKa = 11.84 DD360 pKa = 3.65 HH361 pKa = 7.12 ISRR364 pKa = 11.84 RR365 pKa = 11.84 SAAISRR371 pKa = 11.84 CVIKK375 pKa = 10.88 GPEE378 pKa = 3.81 APTWHH383 pKa = 6.81 SSPLDD388 pKa = 3.72 DD389 pKa = 5.66 LKK391 pKa = 11.4 EE392 pKa = 3.97 KK393 pKa = 10.69 SKK395 pKa = 10.91 QGNFSQFCEE404 pKa = 4.14 VAKK407 pKa = 10.73 FGLPRR412 pKa = 11.84 KK413 pKa = 9.11 EE414 pKa = 3.94 NSEE417 pKa = 4.18 SYY419 pKa = 9.74 TFKK422 pKa = 10.65 FPKK425 pKa = 9.96 DD426 pKa = 3.06 ASTIDD431 pKa = 3.17 TAFYY435 pKa = 10.31 LIQEE439 pKa = 4.42 NGRR442 pKa = 11.84 SSVLDD447 pKa = 3.28 PTTADD452 pKa = 3.56 EE453 pKa = 4.31 EE454 pKa = 4.62 LHH456 pKa = 5.62 TEE458 pKa = 4.27 GMNLLFDD465 pKa = 5.17 PYY467 pKa = 10.98 DD468 pKa = 4.44 DD469 pKa = 4.51 EE470 pKa = 6.63 SSAHH474 pKa = 5.68 YY475 pKa = 8.7 ATVLSGKK482 pKa = 10.14 LIQNSNIDD490 pKa = 3.89 GEE492 pKa = 4.8 TLLLPDD498 pKa = 4.47 PTTGLARR505 pKa = 11.84 TNSRR509 pKa = 11.84 YY510 pKa = 9.78 LQGSVLIRR518 pKa = 11.84 NVLPEE523 pKa = 4.17 FDD525 pKa = 3.2 QHH527 pKa = 6.77 EE528 pKa = 4.27 IRR530 pKa = 11.84 LFPRR534 pKa = 11.84 YY535 pKa = 8.53 PQISRR540 pKa = 11.84 LSASLTLLFNMRR552 pKa = 11.84 QVWIPRR558 pKa = 11.84 FKK560 pKa = 10.91 QKK562 pKa = 10.37 VDD564 pKa = 3.66 EE565 pKa = 4.23 QPKK568 pKa = 10.35 LSNFSWNEE576 pKa = 3.81 GCDD579 pKa = 3.51 GTVPSLNVVTAEE591 pKa = 4.14 SSTNGPAAEE600 pKa = 3.97 QQVILWSSYY609 pKa = 7.78 RR610 pKa = 11.84 HH611 pKa = 5.58 VSNSDD616 pKa = 3.0 RR617 pKa = 11.84 PTVDD621 pKa = 2.39 TVYY624 pKa = 10.86 YY625 pKa = 10.37 YY626 pKa = 10.07 STLEE630 pKa = 3.97 LLFGTRR636 pKa = 11.84 SSMMQTYY643 pKa = 10.32 NLHH646 pKa = 5.83 QLLSLHH652 pKa = 6.42
Molecular weight: 73.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.779
IPC2_protein 5.715
IPC_protein 5.779
Toseland 6.287
ProMoST 6.084
Dawson 6.071
Bjellqvist 6.071
Wikipedia 6.084
Rodwell 6.059
Grimsley 6.491
Solomon 6.071
Lehninger 6.059
Nozaki 6.338
DTASelect 6.532
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.44
Patrickios 3.986
IPC_peptide 6.084
IPC2_peptide 6.44
IPC2.peptide.svr19 6.362
Protein with the highest isoelectric point:
>sp|Q85056|CAPSD_AHV2H Capsid protein OS=Atkinsonella hypoxylon virus (isolate 2H) OX=647331 PE=3 SV=1
MM1 pKa = 7.07 STLLIPQDD9 pKa = 4.27 TIAHH13 pKa = 5.87 TFDD16 pKa = 3.27 EE17 pKa = 4.97 AVASEE22 pKa = 4.4 SNLRR26 pKa = 11.84 IDD28 pKa = 4.26 EE29 pKa = 4.2 VPEE32 pKa = 4.03 NYY34 pKa = 10.08 LEE36 pKa = 4.59 RR37 pKa = 11.84 FIHH40 pKa = 6.43 PSEE43 pKa = 4.38 PEE45 pKa = 3.62 NFEE48 pKa = 4.29 FYY50 pKa = 10.77 SLRR53 pKa = 11.84 DD54 pKa = 2.96 SDD56 pKa = 4.19 IPSKK60 pKa = 10.73 RR61 pKa = 11.84 IPKK64 pKa = 10.14 NGIQVFEE71 pKa = 4.01 NLKK74 pKa = 9.96 YY75 pKa = 9.46 HH76 pKa = 6.24 TNSKK80 pKa = 11.06 DD81 pKa = 3.56 NLYY84 pKa = 10.65 KK85 pKa = 10.52 DD86 pKa = 3.98 QPSSGPSPMRR96 pKa = 11.84 GVANIIRR103 pKa = 11.84 EE104 pKa = 4.22 YY105 pKa = 10.42 FPQYY109 pKa = 11.01 LDD111 pKa = 6.1 DD112 pKa = 4.31 LRR114 pKa = 11.84 TWCRR118 pKa = 11.84 PKK120 pKa = 10.95 SSDD123 pKa = 4.36 DD124 pKa = 4.37 SIFNDD129 pKa = 3.73 FNHH132 pKa = 6.22 EE133 pKa = 3.93 QRR135 pKa = 11.84 ITQPFTEE142 pKa = 4.15 EE143 pKa = 3.77 RR144 pKa = 11.84 EE145 pKa = 4.09 RR146 pKa = 11.84 RR147 pKa = 11.84 LLPLIDD153 pKa = 4.15 HH154 pKa = 7.03 FLGIKK159 pKa = 10.0 PYY161 pKa = 10.62 DD162 pKa = 3.38 IVHH165 pKa = 5.78 YY166 pKa = 9.74 CDD168 pKa = 2.86 TRR170 pKa = 11.84 FYY172 pKa = 10.09 PWKK175 pKa = 10.03 LSTRR179 pKa = 11.84 ADD181 pKa = 3.59 YY182 pKa = 10.26 FHH184 pKa = 6.62 NHH186 pKa = 4.37 SRR188 pKa = 11.84 DD189 pKa = 3.68 RR190 pKa = 11.84 KK191 pKa = 8.09 AHH193 pKa = 5.81 AAKK196 pKa = 10.14 SHH198 pKa = 7.02 PDD200 pKa = 3.31 FATGPTKK207 pKa = 10.32 KK208 pKa = 10.42 SYY210 pKa = 10.66 FINSHH215 pKa = 6.13 LFFDD219 pKa = 4.61 RR220 pKa = 11.84 STVHH224 pKa = 6.64 NIKK227 pKa = 10.5 EE228 pKa = 3.97 YY229 pKa = 10.7 GFPFRR234 pKa = 11.84 PTTDD238 pKa = 2.82 SARR241 pKa = 11.84 NEE243 pKa = 4.18 TLLDD247 pKa = 3.36 LWFKK251 pKa = 10.71 KK252 pKa = 10.78 VPTEE256 pKa = 3.72 LLVRR260 pKa = 11.84 SHH262 pKa = 6.88 ISKK265 pKa = 10.4 RR266 pKa = 11.84 DD267 pKa = 3.6 NLKK270 pKa = 10.07 VRR272 pKa = 11.84 PVYY275 pKa = 9.36 NAPMIYY281 pKa = 9.79 IRR283 pKa = 11.84 IEE285 pKa = 3.88 CMLFYY290 pKa = 10.57 PLLAQARR297 pKa = 11.84 KK298 pKa = 9.35 RR299 pKa = 11.84 DD300 pKa = 3.66 CCIMYY305 pKa = 10.45 GLEE308 pKa = 4.21 TIRR311 pKa = 11.84 GGMNEE316 pKa = 4.62 LEE318 pKa = 4.6 RR319 pKa = 11.84 ISNAFNSFLLIDD331 pKa = 4.18 WSRR334 pKa = 11.84 FDD336 pKa = 3.94 HH337 pKa = 6.72 LAPFTISNFFFKK349 pKa = 10.19 KK350 pKa = 9.4 WLPTKK355 pKa = 10.38 ILIDD359 pKa = 3.39 HH360 pKa = 7.16 GYY362 pKa = 9.68 AQISNYY368 pKa = 8.82 HH369 pKa = 5.92 DD370 pKa = 3.79 HH371 pKa = 5.95 VHH373 pKa = 6.1 SFSAQAQSHH382 pKa = 7.03 GIPMISKK389 pKa = 9.5 EE390 pKa = 4.14 YY391 pKa = 8.18 QTPPEE396 pKa = 3.92 ATVFAKK402 pKa = 10.34 KK403 pKa = 10.25 VLNLISFLEE412 pKa = 3.74 RR413 pKa = 11.84 WYY415 pKa = 10.94 RR416 pKa = 11.84 DD417 pKa = 3.53 MVFVTPDD424 pKa = 2.73 GFAYY428 pKa = 10.29 RR429 pKa = 11.84 RR430 pKa = 11.84 THH432 pKa = 6.41 AGVPSGILMTQFIDD446 pKa = 3.59 SFVNLTILLDD456 pKa = 3.63 GLIEE460 pKa = 4.64 FGFTDD465 pKa = 4.07 EE466 pKa = 5.17 EE467 pKa = 4.4 IKK469 pKa = 10.79 QLLVFIMGDD478 pKa = 3.48 DD479 pKa = 3.72 NVIFTPWTLLKK490 pKa = 10.74 LIEE493 pKa = 4.47 FFDD496 pKa = 3.67 WFAKK500 pKa = 8.65 YY501 pKa = 8.85 TLDD504 pKa = 4.06 RR505 pKa = 11.84 FGMVINISKK514 pKa = 10.55 SAVTSIRR521 pKa = 11.84 RR522 pKa = 11.84 KK523 pKa = 9.97 IEE525 pKa = 3.55 VLGYY529 pKa = 7.72 TNNYY533 pKa = 8.69 GFPTRR538 pKa = 11.84 SISKK542 pKa = 10.27 LVGQLAYY549 pKa = 9.68 PEE551 pKa = 4.23 RR552 pKa = 11.84 HH553 pKa = 5.03 VTDD556 pKa = 4.87 ADD558 pKa = 3.16 MCMRR562 pKa = 11.84 AIGFAYY568 pKa = 10.01 ASCAQSEE575 pKa = 4.66 TFHH578 pKa = 6.83 ALCKK582 pKa = 10.35 KK583 pKa = 9.16 VFQYY587 pKa = 11.09 YY588 pKa = 9.35 FAKK591 pKa = 10.12 TSINEE596 pKa = 3.85 RR597 pKa = 11.84 LILKK601 pKa = 9.13 GRR603 pKa = 11.84 KK604 pKa = 9.12 AEE606 pKa = 3.95 LPGMFFAYY614 pKa = 9.6 PDD616 pKa = 3.53 VSEE619 pKa = 5.43 HH620 pKa = 5.91 IRR622 pKa = 11.84 LDD624 pKa = 3.54 HH625 pKa = 6.68 FPSLSEE631 pKa = 4.1 VRR633 pKa = 11.84 ILLSKK638 pKa = 9.78 FQGYY642 pKa = 9.62 LKK644 pKa = 7.84 EE645 pKa = 4.59 TPFGTIPTFSTPQTLRR661 pKa = 11.84 DD662 pKa = 3.51 QTQQ665 pKa = 2.43
Molecular weight: 77.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.018
IPC2_protein 7.38
IPC_protein 7.307
Toseland 7.234
ProMoST 7.79
Dawson 7.966
Bjellqvist 8.214
Wikipedia 7.907
Rodwell 7.98
Grimsley 7.351
Solomon 8.039
Lehninger 8.053
Nozaki 8.331
DTASelect 8.097
Thurlkill 8.141
EMBOSS 8.214
Sillero 8.39
Patrickios 4.291
IPC_peptide 8.039
IPC2_peptide 7.351
IPC2.peptide.svr19 7.342
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1317
652
665
658.5
75.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.391 ± 0.186
0.911 ± 0.195
5.771 ± 0.264
5.239 ± 0.117
6.606 ± 0.913
3.797 ± 0.076
3.341 ± 0.179
6.378 ± 0.763
4.176 ± 0.929
9.112 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.822 ± 0.189
4.86 ± 0.434
5.998 ± 0.09
3.265 ± 0.172
6.302 ± 0.11
9.719 ± 1.674
6.758 ± 0.396
5.011 ± 0.637
1.063 ± 0.094
4.48 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here