Amino acid dipepetide frequency for Clostridium phage phiCP7R

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.185AlaCys: 0.185 ± 0.201
1.48AlaAsp: 1.48 ± 0.46
2.59AlaGlu: 2.59 ± 0.579
2.405AlaPhe: 2.405 ± 0.567
1.295AlaGly: 1.295 ± 0.463
1.295AlaHis: 1.295 ± 0.504
2.405AlaIle: 2.405 ± 0.961
3.33AlaLys: 3.33 ± 0.688
3.33AlaLeu: 3.33 ± 0.851
1.11AlaMet: 1.11 ± 0.686
2.22AlaAsn: 2.22 ± 0.479
1.665AlaPro: 1.665 ± 0.658
1.11AlaGln: 1.11 ± 0.577
1.48AlaArg: 1.48 ± 0.444
2.59AlaSer: 2.59 ± 0.951
2.59AlaThr: 2.59 ± 0.704
2.59AlaVal: 2.59 ± 0.852
0.74AlaTrp: 0.74 ± 0.308
2.22AlaTyr: 2.22 ± 0.609
0.0AlaXaa: 0.0 ± 0.0
Cys
0.555CysAla: 0.555 ± 0.268
0.37CysCys: 0.37 ± 0.213
0.74CysAsp: 0.74 ± 0.3
0.74CysGlu: 0.74 ± 0.331
0.74CysPhe: 0.74 ± 0.394
1.295CysGly: 1.295 ± 0.474
0.185CysHis: 0.185 ± 0.172
1.11CysIle: 1.11 ± 0.48
1.85CysLys: 1.85 ± 0.809
2.405CysLeu: 2.405 ± 0.522
0.0CysMet: 0.0 ± 0.0
0.74CysAsn: 0.74 ± 0.345
0.74CysPro: 0.74 ± 0.414
0.555CysGln: 0.555 ± 0.356
0.185CysArg: 0.185 ± 0.193
0.185CysSer: 0.185 ± 0.156
0.555CysThr: 0.555 ± 0.33
0.74CysVal: 0.74 ± 0.3
0.0CysTrp: 0.0 ± 0.0
0.925CysTyr: 0.925 ± 0.326
0.0CysXaa: 0.0 ± 0.0
Asp
2.405AspAla: 2.405 ± 0.707
1.48AspCys: 1.48 ± 0.582
3.515AspAsp: 3.515 ± 0.938
5.365AspGlu: 5.365 ± 0.864
2.96AspPhe: 2.96 ± 0.596
4.81AspGly: 4.81 ± 1.249
1.11AspHis: 1.11 ± 0.353
4.995AspIle: 4.995 ± 0.587
7.031AspLys: 7.031 ± 1.28
3.885AspLeu: 3.885 ± 1.056
2.22AspMet: 2.22 ± 0.626
3.885AspAsn: 3.885 ± 0.893
2.035AspPro: 2.035 ± 0.529
0.555AspGln: 0.555 ± 0.313
2.96AspArg: 2.96 ± 0.876
2.035AspSer: 2.035 ± 0.637
3.7AspThr: 3.7 ± 0.899
5.365AspVal: 5.365 ± 1.186
1.11AspTrp: 1.11 ± 0.433
2.96AspTyr: 2.96 ± 0.602
0.0AspXaa: 0.0 ± 0.0
Glu
0.185GluAla: 0.185 ± 0.177
0.555GluCys: 0.555 ± 0.308
3.515GluAsp: 3.515 ± 0.614
6.29GluGlu: 6.29 ± 1.103
3.145GluPhe: 3.145 ± 0.794
6.475GluGly: 6.475 ± 1.26
1.665GluHis: 1.665 ± 0.609
4.625GluIle: 4.625 ± 0.972
7.031GluLys: 7.031 ± 1.392
5.55GluLeu: 5.55 ± 1.003
3.33GluMet: 3.33 ± 1.038
5.55GluAsn: 5.55 ± 0.986
2.035GluPro: 2.035 ± 0.489
2.22GluGln: 2.22 ± 0.603
2.96GluArg: 2.96 ± 0.776
2.96GluSer: 2.96 ± 0.894
3.885GluThr: 3.885 ± 0.964
6.475GluVal: 6.475 ± 0.858
0.74GluTrp: 0.74 ± 0.365
4.44GluTyr: 4.44 ± 0.904
0.0GluXaa: 0.0 ± 0.0
Phe
2.22PheAla: 2.22 ± 0.592
0.555PheCys: 0.555 ± 0.276
4.07PheAsp: 4.07 ± 0.726
4.44PheGlu: 4.44 ± 0.698
2.96PhePhe: 2.96 ± 0.84
2.405PheGly: 2.405 ± 0.668
0.555PheHis: 0.555 ± 0.347
3.7PheIle: 3.7 ± 1.008
3.7PheLys: 3.7 ± 0.781
3.885PheLeu: 3.885 ± 0.612
2.405PheMet: 2.405 ± 0.778
3.7PheAsn: 3.7 ± 0.9
1.665PhePro: 1.665 ± 0.519
0.925PheGln: 0.925 ± 0.368
0.925PheArg: 0.925 ± 0.476
2.035PheSer: 2.035 ± 0.598
1.665PheThr: 1.665 ± 0.756
3.33PheVal: 3.33 ± 0.589
0.37PheTrp: 0.37 ± 0.311
1.85PheTyr: 1.85 ± 0.613
0.0PheXaa: 0.0 ± 0.0
Gly
3.885GlyAla: 3.885 ± 0.756
1.48GlyCys: 1.48 ± 0.459
5.735GlyAsp: 5.735 ± 1.212
3.515GlyGlu: 3.515 ± 0.864
4.625GlyPhe: 4.625 ± 0.93
3.7GlyGly: 3.7 ± 1.146
1.295GlyHis: 1.295 ± 0.431
5.365GlyIle: 5.365 ± 1.156
7.771GlyLys: 7.771 ± 1.402
6.475GlyLeu: 6.475 ± 1.038
2.035GlyMet: 2.035 ± 0.761
6.105GlyAsn: 6.105 ± 1.461
0.0GlyPro: 0.0 ± 0.0
2.22GlyGln: 2.22 ± 0.618
1.85GlyArg: 1.85 ± 0.782
2.775GlySer: 2.775 ± 0.529
3.885GlyThr: 3.885 ± 1.23
5.365GlyVal: 5.365 ± 1.189
1.11GlyTrp: 1.11 ± 0.458
4.625GlyTyr: 4.625 ± 1.04
0.0GlyXaa: 0.0 ± 0.0
His
0.925HisAla: 0.925 ± 0.427
0.37HisCys: 0.37 ± 0.256
1.11HisAsp: 1.11 ± 0.494
0.555HisGlu: 0.555 ± 0.244
1.665HisPhe: 1.665 ± 0.376
1.295HisGly: 1.295 ± 0.37
0.555HisHis: 0.555 ± 0.3
1.295HisIle: 1.295 ± 0.462
1.85HisLys: 1.85 ± 0.706
0.74HisLeu: 0.74 ± 0.419
0.74HisMet: 0.74 ± 0.391
1.48HisAsn: 1.48 ± 0.567
0.37HisPro: 0.37 ± 0.272
0.0HisGln: 0.0 ± 0.0
0.185HisArg: 0.185 ± 0.173
1.295HisSer: 1.295 ± 0.465
0.555HisThr: 0.555 ± 0.347
1.85HisVal: 1.85 ± 0.73
0.185HisTrp: 0.185 ± 0.183
0.74HisTyr: 0.74 ± 0.354
0.0HisXaa: 0.0 ± 0.0
Ile
2.59IleAla: 2.59 ± 0.533
0.74IleCys: 0.74 ± 0.277
6.475IleAsp: 6.475 ± 1.219
7.216IleGlu: 7.216 ± 1.241
2.775IlePhe: 2.775 ± 0.528
6.29IleGly: 6.29 ± 0.762
0.925IleHis: 0.925 ± 0.324
5.18IleIle: 5.18 ± 0.946
6.29IleLys: 6.29 ± 1.045
3.885IleLeu: 3.885 ± 0.857
3.145IleMet: 3.145 ± 0.551
7.216IleAsn: 7.216 ± 1.85
2.405IlePro: 2.405 ± 0.63
2.035IleGln: 2.035 ± 0.737
1.85IleArg: 1.85 ± 0.574
2.59IleSer: 2.59 ± 0.723
5.92IleThr: 5.92 ± 0.716
2.96IleVal: 2.96 ± 0.78
0.0IleTrp: 0.0 ± 0.0
2.775IleTyr: 2.775 ± 0.593
0.0IleXaa: 0.0 ± 0.0
Lys
3.885LysAla: 3.885 ± 0.811
0.925LysCys: 0.925 ± 0.312
6.66LysAsp: 6.66 ± 0.853
8.511LysGlu: 8.511 ± 1.244
3.33LysPhe: 3.33 ± 0.844
6.846LysGly: 6.846 ± 0.798
1.85LysHis: 1.85 ± 0.629
4.995LysIle: 4.995 ± 1.094
6.846LysLys: 6.846 ± 1.944
5.735LysLeu: 5.735 ± 0.948
3.515LysMet: 3.515 ± 0.724
5.55LysAsn: 5.55 ± 1.107
2.405LysPro: 2.405 ± 0.611
4.44LysGln: 4.44 ± 0.96
2.775LysArg: 2.775 ± 0.932
3.145LysSer: 3.145 ± 0.666
4.625LysThr: 4.625 ± 1.389
4.625LysVal: 4.625 ± 1.08
1.48LysTrp: 1.48 ± 0.596
5.735LysTyr: 5.735 ± 1.289
0.0LysXaa: 0.0 ± 0.0
Leu
2.22LeuAla: 2.22 ± 0.749
1.48LeuCys: 1.48 ± 0.334
5.735LeuAsp: 5.735 ± 0.903
4.625LeuGlu: 4.625 ± 0.788
2.59LeuPhe: 2.59 ± 0.583
5.735LeuGly: 5.735 ± 0.956
1.11LeuHis: 1.11 ± 0.616
5.365LeuIle: 5.365 ± 0.924
6.105LeuLys: 6.105 ± 0.96
5.18LeuLeu: 5.18 ± 0.852
2.405LeuMet: 2.405 ± 0.816
5.55LeuAsn: 5.55 ± 0.942
1.11LeuPro: 1.11 ± 0.493
2.96LeuGln: 2.96 ± 1.031
3.145LeuArg: 3.145 ± 0.763
2.59LeuSer: 2.59 ± 0.631
3.515LeuThr: 3.515 ± 0.659
4.81LeuVal: 4.81 ± 0.999
0.74LeuTrp: 0.74 ± 0.298
2.775LeuTyr: 2.775 ± 0.522
0.0LeuXaa: 0.0 ± 0.0
Met
2.035MetAla: 2.035 ± 0.827
0.37MetCys: 0.37 ± 0.247
2.59MetAsp: 2.59 ± 0.68
2.59MetGlu: 2.59 ± 0.805
1.85MetPhe: 1.85 ± 0.43
1.48MetGly: 1.48 ± 0.526
0.0MetHis: 0.0 ± 0.0
2.775MetIle: 2.775 ± 0.56
2.775MetLys: 2.775 ± 0.702
2.405MetLeu: 2.405 ± 0.76
0.185MetMet: 0.185 ± 0.177
2.775MetAsn: 2.775 ± 0.522
1.295MetPro: 1.295 ± 0.433
1.11MetGln: 1.11 ± 0.758
0.555MetArg: 0.555 ± 0.236
2.035MetSer: 2.035 ± 0.748
2.035MetThr: 2.035 ± 0.641
1.295MetVal: 1.295 ± 0.546
0.185MetTrp: 0.185 ± 0.172
2.405MetTyr: 2.405 ± 0.815
0.0MetXaa: 0.0 ± 0.0
Asn
3.145AsnAla: 3.145 ± 0.466
0.74AsnCys: 0.74 ± 0.357
2.96AsnAsp: 2.96 ± 0.835
5.365AsnGlu: 5.365 ± 1.056
2.035AsnPhe: 2.035 ± 0.633
6.66AsnGly: 6.66 ± 1.122
1.11AsnHis: 1.11 ± 0.418
5.92AsnIle: 5.92 ± 1.148
6.29AsnLys: 6.29 ± 0.795
4.625AsnLeu: 4.625 ± 1.025
3.33AsnMet: 3.33 ± 0.862
5.365AsnAsn: 5.365 ± 1.091
1.85AsnPro: 1.85 ± 0.616
2.035AsnGln: 2.035 ± 0.593
3.33AsnArg: 3.33 ± 0.794
4.44AsnSer: 4.44 ± 0.902
4.995AsnThr: 4.995 ± 1.109
3.7AsnVal: 3.7 ± 0.744
1.11AsnTrp: 1.11 ± 0.394
4.625AsnTyr: 4.625 ± 1.211
0.0AsnXaa: 0.0 ± 0.0
Pro
0.74ProAla: 0.74 ± 0.533
0.185ProCys: 0.185 ± 0.201
0.37ProAsp: 0.37 ± 0.231
0.555ProGlu: 0.555 ± 0.319
0.925ProPhe: 0.925 ± 0.411
2.775ProGly: 2.775 ± 0.912
0.37ProHis: 0.37 ± 0.25
1.665ProIle: 1.665 ± 0.425
2.405ProLys: 2.405 ± 0.694
1.665ProLeu: 1.665 ± 0.446
0.555ProMet: 0.555 ± 0.244
1.665ProAsn: 1.665 ± 0.538
0.74ProPro: 0.74 ± 0.344
1.11ProGln: 1.11 ± 0.48
0.185ProArg: 0.185 ± 0.177
1.85ProSer: 1.85 ± 0.507
2.035ProThr: 2.035 ± 0.528
2.96ProVal: 2.96 ± 0.566
0.0ProTrp: 0.0 ± 0.0
2.96ProTyr: 2.96 ± 0.603
0.0ProXaa: 0.0 ± 0.0
Gln
2.59GlnAla: 2.59 ± 1.089
0.74GlnCys: 0.74 ± 0.272
0.74GlnAsp: 0.74 ± 0.413
1.665GlnGlu: 1.665 ± 0.553
1.48GlnPhe: 1.48 ± 0.468
3.145GlnGly: 3.145 ± 0.648
0.37GlnHis: 0.37 ± 0.257
1.85GlnIle: 1.85 ± 0.927
2.59GlnLys: 2.59 ± 1.11
1.665GlnLeu: 1.665 ± 0.689
1.11GlnMet: 1.11 ± 0.455
2.59GlnAsn: 2.59 ± 0.602
0.185GlnPro: 0.185 ± 0.193
1.11GlnGln: 1.11 ± 0.55
1.48GlnArg: 1.48 ± 0.479
1.85GlnSer: 1.85 ± 0.425
1.85GlnThr: 1.85 ± 0.51
0.925GlnVal: 0.925 ± 0.449
0.185GlnTrp: 0.185 ± 0.181
2.22GlnTyr: 2.22 ± 0.462
0.0GlnXaa: 0.0 ± 0.0
Arg
0.925ArgAla: 0.925 ± 0.334
0.74ArgCys: 0.74 ± 0.285
1.665ArgAsp: 1.665 ± 0.597
4.255ArgGlu: 4.255 ± 0.792
1.48ArgPhe: 1.48 ± 0.572
2.96ArgGly: 2.96 ± 0.816
0.555ArgHis: 0.555 ± 0.45
2.96ArgIle: 2.96 ± 0.661
3.33ArgLys: 3.33 ± 0.868
2.96ArgLeu: 2.96 ± 0.972
0.925ArgMet: 0.925 ± 0.505
2.22ArgAsn: 2.22 ± 0.698
0.925ArgPro: 0.925 ± 0.394
0.74ArgGln: 0.74 ± 0.322
2.775ArgArg: 2.775 ± 0.941
1.11ArgSer: 1.11 ± 0.479
2.22ArgThr: 2.22 ± 0.615
2.22ArgVal: 2.22 ± 0.473
1.11ArgTrp: 1.11 ± 0.341
2.405ArgTyr: 2.405 ± 0.6
0.0ArgXaa: 0.0 ± 0.0
Ser
3.145SerAla: 3.145 ± 0.936
0.74SerCys: 0.74 ± 0.268
2.035SerAsp: 2.035 ± 0.636
2.405SerGlu: 2.405 ± 0.846
2.59SerPhe: 2.59 ± 0.685
2.96SerGly: 2.96 ± 0.796
0.555SerHis: 0.555 ± 0.275
4.625SerIle: 4.625 ± 0.921
3.885SerLys: 3.885 ± 0.693
3.33SerLeu: 3.33 ± 0.696
1.665SerMet: 1.665 ± 0.814
3.145SerAsn: 3.145 ± 0.88
1.665SerPro: 1.665 ± 0.526
2.405SerGln: 2.405 ± 0.51
1.85SerArg: 1.85 ± 0.515
5.18SerSer: 5.18 ± 1.404
2.96SerThr: 2.96 ± 0.746
3.33SerVal: 3.33 ± 0.929
0.555SerTrp: 0.555 ± 0.319
2.405SerTyr: 2.405 ± 0.541
0.0SerXaa: 0.0 ± 0.0
Thr
1.295ThrAla: 1.295 ± 0.453
0.925ThrCys: 0.925 ± 0.358
5.18ThrAsp: 5.18 ± 0.736
3.33ThrGlu: 3.33 ± 0.731
2.035ThrPhe: 2.035 ± 0.628
5.55ThrGly: 5.55 ± 0.911
1.295ThrHis: 1.295 ± 0.401
5.55ThrIle: 5.55 ± 0.82
5.18ThrLys: 5.18 ± 0.825
2.405ThrLeu: 2.405 ± 0.475
1.665ThrMet: 1.665 ± 0.937
3.33ThrAsn: 3.33 ± 0.618
1.48ThrPro: 1.48 ± 0.672
2.035ThrGln: 2.035 ± 0.688
1.665ThrArg: 1.665 ± 0.509
4.07ThrSer: 4.07 ± 0.901
3.145ThrThr: 3.145 ± 0.964
3.885ThrVal: 3.885 ± 0.863
0.925ThrTrp: 0.925 ± 0.386
2.775ThrTyr: 2.775 ± 1.021
0.0ThrXaa: 0.0 ± 0.0
Val
1.665ValAla: 1.665 ± 0.511
0.555ValCys: 0.555 ± 0.253
4.995ValAsp: 4.995 ± 0.865
5.18ValGlu: 5.18 ± 1.204
3.33ValPhe: 3.33 ± 0.817
3.33ValGly: 3.33 ± 1.193
1.11ValHis: 1.11 ± 0.344
3.515ValIle: 3.515 ± 0.87
4.44ValLys: 4.44 ± 0.857
4.07ValLeu: 4.07 ± 0.657
0.925ValMet: 0.925 ± 0.414
5.92ValAsn: 5.92 ± 0.91
1.295ValPro: 1.295 ± 0.482
1.295ValGln: 1.295 ± 0.401
3.885ValArg: 3.885 ± 1.011
4.995ValSer: 4.995 ± 1.025
3.885ValThr: 3.885 ± 0.874
2.775ValVal: 2.775 ± 0.67
0.925ValTrp: 0.925 ± 0.433
3.885ValTyr: 3.885 ± 0.817
0.0ValXaa: 0.0 ± 0.0
Trp
0.185TrpAla: 0.185 ± 0.177
0.185TrpCys: 0.185 ± 0.18
0.74TrpAsp: 0.74 ± 0.366
1.295TrpGlu: 1.295 ± 0.316
0.555TrpPhe: 0.555 ± 0.317
0.555TrpGly: 0.555 ± 0.356
0.37TrpHis: 0.37 ± 0.235
1.295TrpIle: 1.295 ± 0.38
0.74TrpLys: 0.74 ± 0.419
1.665TrpLeu: 1.665 ± 0.651
0.0TrpMet: 0.0 ± 0.0
1.11TrpAsn: 1.11 ± 0.402
0.0TrpPro: 0.0 ± 0.0
0.37TrpGln: 0.37 ± 0.221
0.925TrpArg: 0.925 ± 0.35
0.555TrpSer: 0.555 ± 0.301
0.555TrpThr: 0.555 ± 0.261
0.37TrpVal: 0.37 ± 0.281
0.0TrpTrp: 0.0 ± 0.0
0.555TrpTyr: 0.555 ± 0.278
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.035TyrAla: 2.035 ± 0.627
1.295TyrCys: 1.295 ± 0.499
4.07TyrAsp: 4.07 ± 0.98
3.145TyrGlu: 3.145 ± 0.631
3.885TyrPhe: 3.885 ± 0.484
4.255TyrGly: 4.255 ± 0.881
1.48TyrHis: 1.48 ± 0.405
4.07TyrIle: 4.07 ± 0.715
4.44TyrLys: 4.44 ± 0.861
3.885TyrLeu: 3.885 ± 0.544
1.295TyrMet: 1.295 ± 0.393
3.515TyrAsn: 3.515 ± 0.606
1.85TyrPro: 1.85 ± 0.678
1.11TyrGln: 1.11 ± 0.455
3.7TyrArg: 3.7 ± 0.826
3.145TyrSer: 3.145 ± 0.953
3.145TyrThr: 3.145 ± 0.794
2.405TyrVal: 2.405 ± 0.671
0.555TyrTrp: 0.555 ± 0.328
3.515TyrTyr: 3.515 ± 0.835
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 26 proteins (5406 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski