Hyperthermophilic Archaeal Virus 1
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9CGA6|D9CGA6_9VIRU Uncharacterized protein OS=Hyperthermophilic Archaeal Virus 1 OX=762905 GN=HAV1_gp37 PE=4 SV=1
MM1 pKa = 7.27 AVVEE5 pKa = 3.99 GMYY8 pKa = 10.36 YY9 pKa = 11.25 GFMAVAAFIGLLLLLLFFASHH30 pKa = 6.72 AVLTADD36 pKa = 3.46 SMKK39 pKa = 9.68 WRR41 pKa = 11.84 ALAYY45 pKa = 9.45 IVAVFDD51 pKa = 3.17 AWFIGAIFGTLSCNEE66 pKa = 3.89 VCRR69 pKa = 11.84 KK70 pKa = 9.96 GGEE73 pKa = 4.17 DD74 pKa = 4.94 GSAQDD79 pKa = 3.63
Molecular weight: 8.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.401
IPC2_protein 4.813
IPC_protein 4.52
Toseland 4.368
ProMoST 4.431
Dawson 4.495
Bjellqvist 4.749
Wikipedia 4.418
Rodwell 4.368
Grimsley 4.291
Solomon 4.482
Lehninger 4.431
Nozaki 4.622
DTASelect 4.8
Thurlkill 4.406
EMBOSS 4.431
Sillero 4.647
Patrickios 2.041
IPC_peptide 4.482
IPC2_peptide 4.635
IPC2.peptide.svr19 4.672
Protein with the highest isoelectric point:
>tr|D9CGA9|D9CGA9_9VIRU Uncharacterized protein OS=Hyperthermophilic Archaeal Virus 1 OX=762905 GN=HAV1_gp40 PE=4 SV=1
MM1 pKa = 7.19 MCRR4 pKa = 11.84 INLRR8 pKa = 11.84 CFFKK12 pKa = 11.14 SLWNLLKK19 pKa = 10.6 QVGIFIGMIAAVILLAFAVILAIAAVLWFIYY50 pKa = 9.89 FITTLDD56 pKa = 3.51 PMILVAVVVAFLAYY70 pKa = 8.76 VTIASLFRR78 pKa = 11.84 KK79 pKa = 7.5 QTT81 pKa = 3.24
Molecular weight: 9.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.21
IPC2_protein 9.355
IPC_protein 9.502
Toseland 9.926
ProMoST 9.663
Dawson 10.16
Bjellqvist 9.897
Wikipedia 10.335
Rodwell 10.511
Grimsley 10.233
Solomon 10.233
Lehninger 10.204
Nozaki 10.058
DTASelect 9.853
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.101
Patrickios 10.423
IPC_peptide 10.218
IPC2_peptide 9.033
IPC2.peptide.svr19 8.117
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
6403
50
585
160.1
18.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.528 ± 0.655
1.843 ± 0.223
5.06 ± 0.427
6.091 ± 0.806
4.186 ± 0.284
5.279 ± 0.418
1.203 ± 0.169
7.481 ± 0.533
6.31 ± 0.466
8.324 ± 0.354
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.499 ± 0.246
3.373 ± 0.318
4.529 ± 0.405
2.39 ± 0.265
5.95 ± 0.511
6.95 ± 0.95
5.123 ± 0.349
8.527 ± 0.479
1.609 ± 0.232
5.747 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here