Bacillus sp. FJAT-27986

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B9AIM1|A0A1B9AIM1_9BACI Quinone oxidoreductase OS=Bacillus sp. FJAT-27986 OX=1743146 GN=A8L44_12685 PE=4 SV=1
MM1 pKa = 7.56SNLEE5 pKa = 3.95EE6 pKa = 4.05AFKK9 pKa = 10.95LIEE12 pKa = 4.34KK13 pKa = 10.09DD14 pKa = 3.01IEE16 pKa = 4.02FADD19 pKa = 4.38FVGGLSTEE27 pKa = 4.35QIDD30 pKa = 4.26NLQDD34 pKa = 3.4EE35 pKa = 5.33LNLIFPDD42 pKa = 3.85SYY44 pKa = 11.58RR45 pKa = 11.84KK46 pKa = 9.32FLSIYY51 pKa = 10.77GMGDD55 pKa = 2.57IHH57 pKa = 8.15GVEE60 pKa = 4.29IYY62 pKa = 10.79GAGSDD67 pKa = 3.65IDD69 pKa = 3.95NDD71 pKa = 4.35GVPSAIWYY79 pKa = 7.27TKK81 pKa = 9.58FLRR84 pKa = 11.84EE85 pKa = 3.86RR86 pKa = 11.84ANLPLYY92 pKa = 9.74LLPVYY97 pKa = 10.38EE98 pKa = 4.68SGFDD102 pKa = 3.91GEE104 pKa = 5.36IFCIDD109 pKa = 3.79CSNNSSNPIVLFIGGLPIEE128 pKa = 4.29EE129 pKa = 4.43QEE131 pKa = 4.2FEE133 pKa = 4.41VYY135 pKa = 10.44DD136 pKa = 3.83EE137 pKa = 4.53SFGDD141 pKa = 3.94FLYY144 pKa = 10.81HH145 pKa = 6.52LLVMATEE152 pKa = 4.44EE153 pKa = 4.03

Molecular weight: 17.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B9AD08|A0A1B9AD08_9BACI Uncharacterized protein OS=Bacillus sp. FJAT-27986 OX=1743146 GN=A8L44_14055 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.91VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3515

0

3515

1038095

26

1592

295.3

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.759 ± 0.043

0.734 ± 0.012

5.182 ± 0.037

7.485 ± 0.052

4.457 ± 0.035

6.918 ± 0.04

2.032 ± 0.022

8.365 ± 0.044

7.103 ± 0.044

9.617 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.021

4.623 ± 0.027

3.496 ± 0.024

3.525 ± 0.024

3.864 ± 0.035

6.117 ± 0.036

5.423 ± 0.031

6.668 ± 0.035

1.02 ± 0.015

3.759 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski