Streptomyces sp. ICBB 8177

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U3C2V8|A0A2U3C2V8_9ACTN Transcriptional regulator OS=Streptomyces sp. ICBB 8177 OX=563922 GN=CK485_14705 PE=4 SV=1
MM1 pKa = 6.8VHH3 pKa = 6.94SDD5 pKa = 3.28AQIPDD10 pKa = 3.54STTTSVTDD18 pKa = 4.2DD19 pKa = 3.88FGQLFRR25 pKa = 11.84TYY27 pKa = 10.78KK28 pKa = 10.6SYY30 pKa = 11.49VDD32 pKa = 3.6PWNLPDD38 pKa = 3.81SDD40 pKa = 3.93FVLRR44 pKa = 11.84GVQVVSSNEE53 pKa = 3.93GSDD56 pKa = 3.55TTSSVSDD63 pKa = 3.31VDD65 pKa = 4.01ATFTPNADD73 pKa = 3.15PSAAPEE79 pKa = 4.38GPLRR83 pKa = 11.84YY84 pKa = 9.8GIQGSGGSNLCLDD97 pKa = 4.76DD98 pKa = 5.56FGSATQAPATADD110 pKa = 3.23LWDD113 pKa = 4.15CTSGDD118 pKa = 3.78GAQNWTFGLDD128 pKa = 2.98GTLQVNGLCLAADD141 pKa = 4.35GTAAGSAVALASCDD155 pKa = 3.16GTTGQKK161 pKa = 8.91WVTPQQQ167 pKa = 3.24

Molecular weight: 17.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U3BX21|A0A2U3BX21_9ACTN Amino acid permease OS=Streptomyces sp. ICBB 8177 OX=563922 GN=CK485_20750 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5334

0

5334

1750405

29

5314

328.2

35.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.111 ± 0.05

0.838 ± 0.009

5.984 ± 0.027

5.485 ± 0.038

2.671 ± 0.017

9.717 ± 0.029

2.322 ± 0.017

2.882 ± 0.026

1.863 ± 0.024

10.172 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.705 ± 0.014

1.648 ± 0.017

6.275 ± 0.03

2.698 ± 0.022

8.38 ± 0.039

4.969 ± 0.032

6.043 ± 0.03

8.689 ± 0.032

1.475 ± 0.015

2.073 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski