Gigaspora rosea
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31020 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A397U618|A0A397U618_9GLOM Uncharacterized protein (Fragment) OS=Gigaspora rosea OX=44941 GN=C2G38_2117867 PE=4 SV=1
MM1 pKa = 7.71 SDD3 pKa = 3.4 SYY5 pKa = 11.49 HH6 pKa = 6.86 PSLYY10 pKa = 10.51 SYY12 pKa = 11.35 LKK14 pKa = 10.0 DD15 pKa = 3.25 TEE17 pKa = 4.37 NYY19 pKa = 9.97 NFYY22 pKa = 10.76 EE23 pKa = 3.84 FLIIHH28 pKa = 6.61 RR29 pKa = 11.84 NVVVNSPPFNDD40 pKa = 4.95 DD41 pKa = 4.38 LILINGTLGEE51 pKa = 4.18 ITDD54 pKa = 4.21 IVNKK58 pKa = 10.19 FYY60 pKa = 8.07 TTQGTSSQDD69 pKa = 3.1 LDD71 pKa = 3.72 STINPSSSASEE82 pKa = 3.68 GDD84 pKa = 3.25 EE85 pKa = 4.03 ANIIEE90 pKa = 5.11 DD91 pKa = 4.94 LDD93 pKa = 4.06 TSDD96 pKa = 5.45 HH97 pKa = 7.57 DD98 pKa = 4.06 EE99 pKa = 5.74 LSNQDD104 pKa = 3.38 NLVNLEE110 pKa = 4.0 PLRR113 pKa = 11.84 NDD115 pKa = 3.75 SSSQDD120 pKa = 3.76 DD121 pKa = 4.44 LNNMNNLEE129 pKa = 4.64 PPSSHH134 pKa = 7.74 DD135 pKa = 3.93 DD136 pKa = 4.34 LIHH139 pKa = 7.37 LEE141 pKa = 4.73 SLNQDD146 pKa = 2.83 NDD148 pKa = 3.65 SYY150 pKa = 11.28 QVQFIIEE157 pKa = 4.28 KK158 pKa = 9.95 EE159 pKa = 3.78 IMINIILVLVEE170 pKa = 3.96
Molecular weight: 19.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.694
IPC_protein 3.694
Toseland 3.478
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.884
Wikipedia 3.63
Rodwell 3.528
Grimsley 3.389
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.05
Thurlkill 3.541
EMBOSS 3.643
Sillero 3.821
Patrickios 1.036
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A397UBZ8|A0A397UBZ8_9GLOM Uncharacterized protein OS=Gigaspora rosea OX=44941 GN=C2G38_2120278 PE=4 SV=1
MM1 pKa = 7.23 LVVAVSRR8 pKa = 11.84 TALIAAVIAANSVSSLTLRR27 pKa = 11.84 SASSLALRR35 pKa = 11.84 SASSLALRR43 pKa = 11.84 SVSSLTLRR51 pKa = 11.84 SASSLALRR59 pKa = 11.84 SASSLALRR67 pKa = 11.84 SASSLALRR75 pKa = 11.84 SASSLALRR83 pKa = 11.84 SASSLALRR91 pKa = 11.84 SASSLALRR99 pKa = 11.84 LASSLLYY106 pKa = 10.51 VQLPLL111 pKa = 4.99
Molecular weight: 11.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.501
Toseland 12.647
ProMoST 13.159
Dawson 12.647
Bjellqvist 12.647
Wikipedia 13.13
Rodwell 12.149
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.647
DTASelect 12.647
Thurlkill 12.647
EMBOSS 13.159
Sillero 12.647
Patrickios 11.901
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.141
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
31016
4
31020
9364735
49
5216
301.9
34.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.792 ± 0.012
1.662 ± 0.007
5.654 ± 0.011
6.764 ± 0.016
4.683 ± 0.011
4.588 ± 0.015
2.156 ± 0.007
8.185 ± 0.014
7.725 ± 0.017
9.407 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.039 ± 0.006
6.905 ± 0.015
3.944 ± 0.013
3.762 ± 0.011
4.254 ± 0.012
7.798 ± 0.016
5.468 ± 0.012
5.031 ± 0.011
1.168 ± 0.005
4.015 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here